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Summer mortality outbreaks of French Pacific oysters Crassostrea gigas in 2008 and 2009 ArchiMer
Renault, Tristan; Allain, Gwenhael; Arzul, Isabelle; Chollet, Bruno; Cobret, Laetitia; De Decker, Sophie; Faury, Nicole; Ferrand, Sylvie; Francois, Cyrille; Garcia, Celine; Haffner, Philippe; Joly, Jean-pierre; Miossec, Laurence; Morga, Benjamin; Nicolas, Jean-louis; Omnes, Emmanuelle; Pepin, Jean-francois; Saulnier, Denis; Schikorski, David; Segarra, Amelie.
Abnormal mortality outbreaks occurred in the majority of Pacific oyster (Crassostrea gigas) producing areas in France in 2008 during the summer period. These outbreaks were usually sudden and severe (up to 100%) and affected essentially spat (oysters less than 1 year-old) and juveniles (12 to 18 month-old oysters). Crassostrea gigas was the single affected species Mortality outbreaks were first observed from mid May to mid June in Brittany, Vendée, Charente Maritime and Mediterranean coast A second episode of massive mortality events was reported at the end of June and the beginning of July. All oyster production sites were affected except some particular locations including Arcachon Bay and Brittany. Mortality occurred latter in Arcachon Bay in August....
Tipo: Text Palavras-chave: Abnormal mortality; Oysters; Crassostrea gigas; Pathogens.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00014/12554/9420.pdf
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Phenotypic and genetic consequences of size selection at the larval stage in the Pacific oyster (Crassostrea gigas) ArchiMer
Taris, Nicolas; Ernande, Bruno; Mccombie, Helen; Boudry, Pierre.
The life histories of oysters in the genus Crassostrea, like those of most marine bivalves, are typified by high fecundity and low survival in nature. Rearing conditions in hatcheries however ensure optimized density, diet, and temperature. Hatcheries are becoming increasingly important for the production of juveniles in aquaculture, and their culture practices often include culling of slow growing larvae to reduce and synchronize the time taken to reach settlement. Because previous studies have found substantial genetic variation for early life developmental traits in Crassostrea gigas, these culling practices are likely to cause highly different selective pressures in hatcheries from those in the natural environment. We studied the phenotypic and genetic...
Tipo: Text Palavras-chave: Oysters; Larvae; Hatchery; Genetic diversity; Culling; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1677.pdf
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Guide du télécaptage de larves d'huîtres Crassostrea gigas ArchiMer
Joly, Jean-pierre; Bodoy, Alain; Baud, Jean-pierre.
Ce guide, destiné aux ostréiculteurs désireux de s'initier au télécaptage de larves d'huîtres Crassostrea gigas, est basé sur la traduction d'une publication canadienne enrichie des quelques premières expériences réalisées en FRANCE. La méthode et les diverses manipulations sont expliquées étape par étape et accompagnées par de nombreux conseils.
Tipo: Text Palavras-chave: Télécaptage; Huître creuse; Crassostrea gigas; Méthode.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/00048/15901/13329.pdf
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Single Nucleotide polymorphisms and their relationship to codon usage bias in the Pacific oyster Crassostrea gigas ArchiMer
Sauvage, Christopher; Bierne, N; Lapegue, Sylvie; Boudry, Pierre.
DNA sequence polymorphism and codon usage bias were investigated in a set of 41 nuclear loci in the Pacific oyster Crassostrea gigas. Our results revealed a very high level of DNA polymorphism in oysters, in the order of magnitude of the highest levels reported in animals to date. A total of 290 single nucleotide polymorphisms (SNPs) were detected, 76 of which being localised in exons and 214 in non-coding regions. Average density of SNPs was estimated to be one SNP every 60 bp in coding regions and one every 40 bp in non-coding regions. Non-synonymous substitutions contributed substantially to the polymorphism observed in coding regions. The non-synonymous to silent diversity ratio was 0.16 on average, which is fairly higher to the ratio reported in other...
Tipo: Text Palavras-chave: Crassostrea gigas; Codon bias; Genetic diversity; SNP.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3417.pdf
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Observations préliminaires sur la présence de Mytilicola orientalis Mori (1935) chez Crassostrea gigas Thunberg dans le bassin d'Arcachon ArchiMer
His, Edouard.
Le copépode parasite Mytilicola orientalis Mori (1935) est signalé pour la première fois en Europe, chez les huîtres Crassostrea gigas Thunberg du Bassin d'Arcachon.
Tipo: Text Palavras-chave: Crassostrea gigas; Mytilicola orientalis; Copépode parasite; Huîtres creuses.
Ano: 1977 URL: http://archimer.ifremer.fr/doc/00016/12734/9665.pdf
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Rapport régional du REPAMO 1999: résultats de la cellule de veille zoosanitaire de La Tremblade dans les régions du sud de la Loire ArchiMer
Thebault, Anne; Chollet, Bruno; Robert, Maeva.
La cellule de veille zoosanitaire de La Tremblade s'occupe de trois zones situées au sud de la Loire: la Baie de Bourgneuf, la région de Marennes-Oléron-Charentes, et le bassin d'Arcachon. Ces régions produisent principalement des huîtres creuses. Le naissain d'huîtres creuses produit à Arcachon et en Charente représente près de 80% de la production d'huître creuse, qui sera élevée dans toutes les régions ostréicoles françaises. Un effort particulier avec des prélèvements systématiques a donc été mis en place sur Crassostrea gigas dans ces trois trois zones, notamment sur les larves et le naissain d'Arcachon et de Charente. Compte tenu des transferts fréquents, des animaux sauvages sont prélevés pour caractériser les sites, tandis que la production en...
Tipo: Text Palavras-chave: Réseau surveillance; REPAMO; Analyses zoosanitaires; Huître creuse; Crassostrea gigas; Mortalités.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00134/24488/22513.pdf
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First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway ArchiMer
Montagnani, Caroline; Avarre, Jean-christophe; De Lorgeril, Julien; Quiquand, M; Boulo, Viviane; Escoubas, Jean-michel.
In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound heating and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and...
Tipo: Text Palavras-chave: Damage associated molecular pattern; Tissue inhibitor of metalloproteinase; Metalloproteinase; Mollusk immunity; Crassostrea gigas; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2094.pdf
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Mortalité de l'huître Crassostrea gigas dans le bassin de Marennes-Oléron: étude de la variabilité spatiale de son environnement et de sa biologie par un système d'informations géographiques (SIG) ArchiMer
Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Razet, Daniel; Geairon, Philippe; Goulletquer, Philippe.
The summer mortality patterns was studied in 1996 on the Ronce-les-Bains oyster bank (175 ha), located on the southern part of the Bay Marennes-Oleron (Atlantic coastline). Studies on growth, sexual maturation, survival rates and environmental variables were compared at fifteen experimental sites (on- and off-bottom culture). After 7 months, cumulative mortality reached 8-19 and 23-33% for off-bottom and on-bottom culture, respectively which is significantly higher for the latter case. A seasonal mortality trend was noted, with a significant increase related to air temperature greater than 20 degrees C and with a significant pre-spawning glycogen catabolism. Based on soft tissue flesh growth results from both types of culture, several empirical models were...
Tipo: Text Palavras-chave: Atlantic Ocean; Crassostrea gigas; Mortality; Growth rates; Océan Atlantique; Crassostrea gigas; Mortalité; Croissance.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-862.pdf
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Impact des facteurs anthropiques sur le recrutement de l'huître : l'exemple du bassin d'Arcachon ArchiMer
His, Edouard; Robert, Rene.
"Il est impossible d'imaginer un lieu plus favorable à l'ostréiculture que le bassin d'Arcachon" (Yonge, 1950). En effet, l'huître indigène, Ostrea edulis, et l'espèce plus récemment introduite, Crassostrea gigas, peuvent s'y reproduire en abondant. Si l'exploitation de la première a pris fin à la suite de deux épizooties consécutives, la seconde est l'objet d'une culture intensive dont la production annuelle est d'environ 12 000 tonnes de mollusques commercialisés. Des anomalies ont sévi dans la baie, de 1977 à 1981, rendant les récoltes de naissain pratiquement sensibles aux facteurs d'agression, et ont même été proposées par Woelke (1967) comme matériel biologique, afin de tester la qualité des eaux en zone littorale. La technique des élevages larvaires...
Tipo: Text Palavras-chave: Bassin d'Arcachon; Crassostrea gigas; Larves; Température; Nutrition; Bioessais; Facteurs anthropiques.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00019/13055/10013.pdf
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Impact of climatic and anthropogenic changes on living resources in the Bay of Biscaye ArchiMer
Goulletquer, Philippe.
Fisheries and aquaculture are of major importance to the coastal communities throughout the Bay of Biscaye. Almost half of French fishermen rely on catches from the Bay which achieve and estimated first sale value of more than €525m annually. Moreover, emblematic species such as eels have an additional economic as weil as social value for coastal communities. Similarly, the main French shellfish rearing areas are located within the Bay of Biscaye. They contribute predominantly to the yearly spat (mussel & oyster) recruitment, used to sustain the entire French shellfish industry. These activities have a direct impact on ecosystem functioning and biodiversity. Meanwhile, drastic environmental changes have been reported over the last 30 years, including a...
Tipo: Text Palavras-chave: Crassostrea gigas; Solea solea; Living resources; Ecosystem; Biodiversity; Impacts; Climate change.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3488.pdf
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Etude de l'herpès virus de l'huître. Fonds d'Aide à la Recherche et à l'Innovation. Décision d'Aide à la Recherche n° 95-07-01 (Rapport intermédiaire) ArchiMer
Renault, Tristan.
L'Unité de Recherche en Pathologie et Immunologie Générales (Station IFREMER de La Tremblade) se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occassionne. Les diagnostics proposés seront basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR : Polymerase Chain Reaction). Ce projet, proposé sur une durée de trois années...
Tipo: Text Palavras-chave: Virus; Herpès; Pathogène; Purification; Herpesviridae; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00043/15447/12818.pdf
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Description, évolution et importance des différentes techniques de captage en rivière Seudre (bassin de Marennes-Oléron) : incidence sur la production d'huîtres creuses C. gigas ArchiMer
Berthome, Jean-paul; Razet, Daniel; Garnier, Jacqueline.
Dealing with the study of the oyster stocks in Marennes Oleron basin, a method is set up to study oystr origin. Various collectors, factory-made or hand-made (about 90% of the whole) are described. Technical evolution, bound to the importance of potential catching in three concerned districts depands on the catching ability of the different types of collectors. The catching, achieved in the Seudre river, represents nearly 1 400 million units, wich is a theorical potentail quantity of 30 000 tons of adults oysters
Tipo: Text Palavras-chave: Larvae; Recrutment; Crassostrea gigas; Oysters; Oyster stock; Technique captage; Recrutement; Crassostrea gigas; Huîtres; Evaluation stocks.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/acte-2935.pdf
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Optimisation of the traditional Pacific cupped oyster (Crassostrea gigas Thunberg) culture on the French Atlantic coastline: autumnal fattening in semi-closed ponds ArchiMer
Soletchnik, Patrick; Le Moine, Olivier; Goulletquer, Philippe; Geairon, Philippe; Razet, Daniel; Faury, Nicole; Fouche, Dominique; Robert, Stephane.
Oyster farming in France is a traditional activity. Each year, 60,000 tons of C. gigas are fattened before being sold. Present-day fattening techniques for improving oyster taste and colour and increasing the meat weight are not particularly reliable. To optimize the fattening process, large phytoplanktonic blooms were induced in ponds, then distributed in oyster ponds. Despite the variability of the autumn weather conditions, diatoms (Skeletonema costatum) have been successfully cultured in outdoor ponds. During neap tides (when no seawater replenishes the water in the oyster beds), between mid-October and mid-December 1996, the fattening index (the weight of the meat) was constant or increased by 10-15% when a supplement of algae of 380.000...
Tipo: Text Palavras-chave: Fattening process; Crassostrea gigas; Oyster farming; Marennes Oleron Bay.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-478.pdf
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Etude de la réponse immunitaire chez l'huître Crassostrea gigas : caractérisation et fonctions de protéines de reconnaissance aux LPS et d'effecteurs antimicrobiens ArchiMer
Gonzalez, Marcelo.
Research on the immune response of oysters is motivated by the problems of infectious diseases potentially found in the farms. Over the course of the last few years, the development of genomic approaches has allowed us to make headway on characterising genes involved in the cup oyster's (Crassostrea gigas) innate immunity, particularly thanks to various programmes for analysing transcriptome, of type EST (Express Sequence Tag) and subtractive banks. Within the context of this work, over the course of which we have used the data from genomic research and we have also developed biochemical approaches, we were particularly interested in studying the proteins involved in reconnaissance reactions to LPS as well as to antimicrobial effectors. In an earlier part,...
Tipo: Text Palavras-chave: Defense peptides; Polysaccharides; LPS; Hemocytes; Immune response; Crassostrea gigas; Shellfish culture; LPS; Défensine; Peptides antimicrobiens; LBP; BPI; Superoxide dismutase; Hémocytes; Crassostrea gigas; Huître; Mollusque; Immunité.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-2098.pdf
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Programme Charente-Marennes-Oléron Volet Maritime ArchiMer
Heral, Maurice; Bacher, Cedric; Razet, Daniel; Prou, Jean; Deslous-paoli, Jean-marc; De Pontual, Helene; Raillard, Olivier; Maestrini, Serge; Mornet, Francoise; Menesguen, Alain; Sautour, Benoit; Robert, Jean-michel.
Les termes du contrat passé entre le Fond de la Recherche et de la Technologie et l'IFREMER précisent qu ele programmer a pour objectif une gestion rationnelle du milieu conchylicole avec notamment une mise au point de modèles (voir notification de l'aide en annexe). Un premier modèle appelé modèle global par analogie avec les modèles de pêche met en évidence la limite de la capacité trophique du bassin de Marennes-Oléron pour les huîtres cultivées. Un modèle analytique avec un modèle physique simple permet dans un premier temps de développer un modèle de croissance des huîtres en fonction de la nourriture disponible et d'effectuer des simulations qui confirment l'influence du stock sur les performances de croissance des bivalves. Un troisième approche...
Tipo: Text Palavras-chave: Modèle production; Biomasse; Huître creuse; Crassostrea gigas; Modèle énergétique; Croissance; Modèle multiboîte; Marennes Oléron; Modèle trophique; Marennes Oléron.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/00048/15908/13337.pdf
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Farmed bivalve loss due to seabream predation in the French Mediterranean Prevost Lagoon ArchiMer
Richard, Marion; Forget, Fabien; Mignucci, Alexandre; Mortreux, Serge; Le Gall, Patrik; Callier, Myriam; Weise, Am; Mckindsey, Cw; Bourjea, Jerome.
Bivalve predation by seabream has been observed worldwide and is a major concern for bivalve farmers. Farmed bivalve-seabream interactions must be better understood to ensure the sustainability of bivalve aquaculture. The objectives of this study were to characterize gilthead seabream Sparus aurata presence in a bivalve farm in Prevost Lagoon (Mediterranean Sea) using acoustic telemetry and to evaluate monthly losses of mussels Mytilus galloprovincialis and oysters Crassostrea gigas due to seabream predation over an 18 mo period inside the farm and at an unprotected experimental platform. Large (281 to 499 mm TL) seabream were more commonly detected in the bivalve farm than were small (200 to 280 mm TL) seabream. In contrast to small seabream, 90% of large...
Tipo: Text Palavras-chave: Shellfish culture; Sparus aurata; Size; Acoustic telemetry; Crassostrea gigas; Mytilus galloprovincialis; Oyster; Mussel.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00663/77515/79299.pdf
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Seuil de production de pseudo-fécès de l'huître creuse du Pacifique : Crassostrea gigas (Thunberg) dans des conditions de faibles charges particulaires ArchiMer
Soletchnik, Patrick; Goulletquer, Philippe; Geairon, Philippe; Razet, Daniel.
This study is part of a comprehensive research program focusing on the oyster shellfish management in the Marennes-Oleron Bay: Within this program, a task aimed to develop a biological model of the Pacific cupped oyster Crassostrea gigas. Our study addressed the issue of pseudo-faeces production threshold under various diet quality levels. Pseudo-faeces sampling was carried out using particulate matter concentration below Il mg 1-1 The various modalities prompted us to define two pseudo-faeces production models. The first model was developed using natural seawater. The organic matter ratio ranged from 25 to 45% while the pseudo-faeces production threshold reached 2.9 to 4.4 mg 1-1 of total particulate matter. The second model was established using seawater...
Tipo: Text Palavras-chave: Marennes Oleron; Pseudo faeces; Crassostrea gigas; Oysters; Biological model; Marennes Oléron; Pseudo fécès; Crassostrea gigas; Huîtres; Modèle biologique.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/publication-3087.pdf
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Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas) ArchiMer
Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
The most significant genetic improvement for production of Pacific oyster (Crassastrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have slowed the development of selective breeding programs. Recently, in the USA, Australia and New Zealand, family-based selective breeding programs have been initiated to improve growth and yield. In Europe, where both natural and...
Tipo: Text Palavras-chave: Microsatellites; Quantitative genetic; Genetic variability; Crassostrea gigas; Oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-2861.pdf
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Characterization of Vibrio isolated from Crassostrea gigas spat suffering summer mortality outbreaks (slides) ArchiMer
Gay, Melanie; Renault, Tristan; Le Roux, Frédérique.
The pathogens related to summer mortality outbreaks are a herpes virus and two bacterial strains one belonging to Vibrio splendidus biovar II and the other to Vibrio splendidus spp. However, the feature pathogen/opportunist of these strains is still unknown. Several strains belonging to the genus Vibrio have been identified as pathogen for different mollusk species. In the context of the French program Morest, experiments of cohabitation have been used to demonstrate the potential presence of a transmissible infectious agent in batches of oysters suffering from summer mortality outbreaks. More than one hundred Vibrio strains have been isolated from these experiments. These strains have been phenotypically and genotypically characterized. Their virulence...
Tipo: Text Palavras-chave: Vibrio splendidus; Vibrio lentus; Crassostrea gigas; Summer; Spat; Pathogen; Disease transmission; Oyster culture; Mortality; Microbial contamination; Oysters.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3343.pdf
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Influence des facteurs climatiques sur la production conchylicole du bassin de Marennes-Oléron ArchiMer
Heral, Maurice; Prou, Jean; Deslous-paoli, Jean-marc.
The authors are comparing the recruitment, the commercialised production, the cultivated biomasses of oysters Crassostrea gigas and Crassostrea angulata with the statistics of the meteorology (1885-1984). The temperature plays a role up on the date of spawning but partialy explains the lack of recruitment, which has less impact on the commercialised production than on the total biomass. The annual and seasonal characteristics of the precipitations and water temperatures do not show tight correlations with the variations of the production and of the biomass, for these cultivated populations in opposition with the sensitivity of the natural molluscan populations towards the climatic factors.
Tipo: Text Palavras-chave: Meteorology; Recruitment; Biomass; Production; Crassostrea angulata; Crassostrea gigas; Météorlogie; Recrutement; Biomasse; Production; Crassostrea gigas; Crassostrea angulata.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/publication-2968.pdf
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