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Registros recuperados: 13
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Molecular characterization of Moenkhausia (Pisces: Characiformes) populations with different lateral line developmental levels Anais da ABC (AABC)
MOTA,THAÍS F.M.; FABRIN,THOMAZ M.C.; DEPRÁ,GABRIEL C.; GASQUES,LUCIANO S.; OLIVEIRA,ALESSANDRA V. DE; PAVANELLI,CARLA S.; PRIOLI,SÔNIA M.A.P.; PRIOLI,ALBERTO J..
ABSTRACT The genera Hemigrammus and Moenkhausia have been traditionally diagnosed mainly by the former having lateral line completely pored whereas the latter having a lateral line with a few pored scales. Those features have been used to diagnose species of both genera in the upper Paraná River floodplain. Specimens with the diagnostic features of Moenkhausia bonita, collected in the upper Paraná River floodplain, exhibited different developmental levels of the lateral line, making it difficult to distinguish them from specimens of Hemigrammus sp. We analyzed the gene encoding cytochrome C oxidase I (COI) and intron 1 of the nuclear gene S7 to investigate the genetic similarities between the called Hemigrammus marginatus and M. bonita and to confirm their...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytochrome C oxidase; Delimitation of species; DNA barcode; Hemigrammus; Intron 1 of the nuclear gene S7; Paraná River.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602815
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Isoenzyme and molecular approach for authenticating and monitoring of animal cell lines Anais da ABC (AABC)
ARAÚJO,SHIRLEY B. DE; PATRICIO,GIANCARLO F.; SIMONI,ISABELA C.; RIVAS,ELIANA B.; FERNANDES,MARIA JUDITE B..
Abstract: Authentication of cell lines is of paramount importance to validate the results from their use in biomedical research. Although isoenzyme polymorphism is the standard method, molecular methods based on mitochondrial DNA (mtDNA) have been developed to replace it. The aim of this study was the improvement of our isoenzyme electrophoretic analysis and the validation of one molecular technique targeted at mtDNA for the authentication of our animal cell lines. The combined method of cellular lysing through osmotic shock, followed by freezing-thawing in N2 to obtain isoenzyme extracts, and with 42 × 106 cells maintained the best efficiency. The superior electrophoretic conditions were PAGE run at 200 V. All cell lines had isoenzymatic mobility...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Authentication; Cell line; DNA barcode; Isoenzyme.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500625
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Diversity of Biomphalaria spp. freshwater snails and associated mollusks in areas with schistosomiasis risk, using molecular and spatial analysis tools Biota Neotropica
Palasio,Raquel Gardini Sanches; Xavier,Iara Giordano; Chiaravalotti-Neto,Francisco; Tuan,Roseli.
Abstract: The Middle Paranapanema River region of São Paulo, Brazil is home to significant diversity of Biomphalaria species and is very vulnerable to health and environmental impacts such as schistosomiasis. This study updates freshwater malacological surveys for ecosystems in one portion of the Middle Paranapanema River Basin, with emphasis on the genus Biomphalaria. Snails were collected from 114 distinct bodies of water between 2015 and 2018. Biomphalaria specimens were identified according to morphological and molecular characteristics, while animals in other genera (Drepanotrema, Lymnaea, Melanoides, Physa and Pomacea) were identified solely according to shell characteristics. A geographic information system was used to update intermediate host...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomphalaria; Freshwater snails; Biodiversity; DNA barcode; Distribution.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032019000400206
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Genetic analysis reveals candidate species in the Scinax catharinae clade (Amphibia: Anura) from Central Brazil Genet. Mol. Biol.
Nogueira,Lídia; Solé,Mirco; Siqueira,Sérgio; Affonso,Paulo Roberto Antunes de Mello; Strüssmann,Christine; Sampaio,Iracilda.
Abstract Scinax (Anura: Hylidae) is a species-rich genus of amphibians (113 spp.), divided into five species groups by morphological features. Cladistic analyses however revealed only two monophyletic clades in these groups: Scinax catharinae and Scinax ruber. Most species from the S. catharinae clade are found in Atlantic rainforest, except for Scinax canastrensis,S. centralis, S. luizotavioi, S. machadoi,S. pombali and S. skaios. In the present work, specimens of Scinax collected in Chapada dos Guimarães, central Brazil, were morphologically compatible with species from theS. catharinae group. On the other hand, genetic analysis based on mitochondrial (16S and 12S) and nuclear (rhodopsin) sequences revealed a nucleotide divergence of 6 to 20% between...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Amphibians; DNA barcode; Cerrado; RDNA.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100049
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Proposal of a trophic relationship between Chaetognatha and Cnidaria based on Mitochondrial COI sequences ArchiMer
Da Mota Melo, Danielle Caroline; Costa Brandao, Manoela; De Albuquerque, Simone Maria; Neumann-leitão, Sigrid.
This is the first study in Brazil to propose a trophic relationship between Chaetognatha and Cnidaria based on molecular data (COI gene - DNA barcode). Cnidaria COI sequences were detected in tissue samples from two specimens of Flaccisagitta enflata collected in the surroundings of Fernando de Noronha archipelago, Tropical Western Atlantic. The methodology used followed all the requirements for a safe molecular manipulation, making the possibility of contamination unlikely. A search carried in BLAST showed that both sequences provided here presented a combination of highest query coverage and match identity with Nemopilema nomurai (Cnidaria: Scyphozoa), supporting the hypothesis that they belong to a species of jellyfish. Since gelatinous taxa are known...
Tipo: Text Palavras-chave: DNA barcode; Food item; Oceanic island; Tropical Atlantic; Zooplankton; Cytochrome oxidase subunit I gene.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00628/74057/73490.pdf
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DNA barcodes, expanded distribution, and redescription of Apanteles hemara Nixon, 1965 (Hymenoptera, Braconidae, Microgastrinae), a potential biocontrol species against amaranth leaf-webbers in Africa Naturalis
Fernandez-Triana, J.; Beaudin, M.; Achterberg, K. van; Agbodzavu, M.K.; Othim, S.T.O.; Nyamu, F.W.; Fiaboe, K.K.M..
The microgastrine parasitoid wasp Apanteles hemara Nixon, 1965, is currently being considered as a potential biocontrol agent of amaranth leaf-webber pests in Africa. To facilitate future research and identification of the species, we characterize it from an integrative taxonomy perspective by providing a comprehensive morphological redescription, extensive illustrations (including the first images of the holotype), DNA barcodes, wasp biology, host data (Choreutidae and Crambidae caterpillars), and updated geographical distribution of the species (including eight new country records). Despite a wide distribution across four major biogeographical regions (mostly within the Old World tropics), the species seems to be relatively uniform from a molecular and...
Tipo: Article / Letter to the editor Palavras-chave: Microgastrinae; Amaranthus; Biocontrol; DNA barcode; Geographical distribution; Africa.
Ano: 2017 URL: http://www.repository.naturalis.nl/record/643636
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A troglomorphic spider from Java (Araneae, Ctenidae, Amauropelma) Naturalis
Miller, J.; Rahmadi, C..
A new troglomorphic spider from caves in Central Java, Indonesia, is described and placed in the ctenid genus Amauropelma Raven, Stumkat & Gray, until now containing only species from Queensland, Australia. Only juveniles and mature females of the new species are known. We give our reasons for placing the new species in Amauropelma, discuss conflicting characters, and make predictions about the morphology of the as yet undiscovered male that will test our taxonomic hypothesis. The description includes DNA barcode sequence data.
Tipo: Article / Letter to the editor Palavras-chave: Conservation; DNA barcode; Indonesia; Jonggrangan Limestone; Troglobite; 42.74.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/434474
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Phylogenetic reassessment of the Chaetomium globosum species complex Naturalis
Wang, X.W.; Lombard , L.; Groenewald, J.Z.; Li, J.; Videira, S.I.R.; Samson, R.A.; Liu, X.Z.; Crous, P.W..
Chaetomium globosum, the type species of the genus, is ubiquitous, occurring on a wide variety of substrates, in air and in marine environments. This species is recognised as a cellulolytic and/or endophytic fungus. It is also known as a source of secondary metabolites with various biological activities, having great potential in the agricultural, medicinal and industrial fields. On the negative side, C. globosum has been reported as an air contaminant causing adverse health effects and as causal agent of human fungal infections. However, the taxonomic status of C. globosum is still poorly understood. The contemporary species concept for this fungus includes a broadly defined morphological diversity as well as a large number of synonymies with limited...
Tipo: Article / Letter to the editor Palavras-chave: DNA barcode; Epitypification; Multi-gene phylogeny; Species complex; Systematics.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/607374
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A new leafminer on grapevine and Rhoicissus (Vitaceae) in South Africa within an expanded generic concept of Holocacista (Insecta, Lepidoptera, Heliozelidae) Naturalis
Nieukerken, E.J. van; Geertsema, H..
A grapevine leafminer found recently in table grape orchards and vineyards in the Paarl region (Western Cape, South Africa) is described as Holocacista capensis sp. n. It has also been found on native Rhoicissus digitata and bred on that species in the laboratory. It is closely related to Holocacista salutans (Meyrick, 1921), comb. n. (from Antispila), described from Durban in KwaZulu-Natal, but widespread in southern Africa and a native leafminer of various Vitaceae: Rhoicissus tomentosa, R. digitata, R. tridentata and Cissus cornifolia. Holocacista capensis has been found on Vitis vinifera both in Gauteng and Western Cape, the earliest record being from 1950 in Pretoria. The initial host shift from native Vitaceae to Vitis must have occurred much...
Tipo: Article / Letter to the editor Palavras-chave: Host shift; Leafminers; Vitaceae; Viticulture; Table grapes; Rhoicissus; Cissus; DNA barcode; Genitalia; Holocacista; Antispilina; Antispila; Afrotropics; South Africa; Zimbabwe; India.
Ano: 2015 URL: http://www.repository.naturalis.nl/record/648754
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Barcoding lepidoptera: current situation and perspectives on the usefulness of a contentious technique Neotropical Entomology
Silva-Brandão,Karina L; Lyra,Mariana L; Freitas,André V L.
Faced by a growing need of identification and delimitation of new and established cryptic species that are being lost at an increasing rate, taxonomists can now more than ever take advantage of an enormous variety of new molecular and computational tools. At this moment they should be open to all new available technologies in the so called "technology-driven revolution" in systematics. The use of the "DNA barcode" has been discussed by those applying successfully this approach to identify and diagnose species and by those who believe that the flaws in the use of this molecular marker are as many as to negate the worth of its employment. For insects of the order Lepidoptera neither side seems totally correct orwrong, and although many groups of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodiversity; Cryptic species; DNA barcode; Systematics; Taxonomy.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000400001
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Molecular authentication of Pargo fillets Lutjanus purpureus (Perciformes: Lutjanidae) by DNA barcoding reveals commercial fraud Neotropical Ichthyology
Veneza,Ivana; Silva,Raimundo; Freitas,Leilane; Silva,Sâmia; Martins,Kely; Sampaio,Iracilda; Schneider,Horacio; Gomes,Grazielle.
ABSTRACT The Caribbean Red Snapper (Pargo) Lutjanus purpureus is the most economically important snapper in Brazil, which is sold, among other forms, as frozen fillets. During the process of transformation into fillets there is the removal of the distinctive morphological traits, being able to favor the substitution by less valued species. In addition, there is no national legislation requiring the insertion of the specific name on the product label. However, according to a Normative Instruction (IN N ° 29/2015 MAPA) that correlates the common and specific names of the products destined to the national trade, in Brazil only L. purpureus and L. campechanus can be denominated “Pargo”. Thus, the DNA barcode tool was used to identify the fillets sold in north...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Commercial fraud; DNA barcode; Fillets; Processed fish; Snapper.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100211
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A new species of Characidium (Characiformes: Crenuchidae) from coastal basins in the Atlantic Rainforest of eastern Brazil, with phylogenetic and phylogeographic insights into the Characidium alipioi species group Neotropical Ichthyology
Malanski,Evandro; Sarmento-Soares,Luisa Maria; Silva-Malanski,Ana Cecilia Gomes; Lopes,Maridiesse Morais; Ingenito,Leonardo Ferreira da Silva; Buckup,Paulo Andreas.
ABSTRACT A new species of Characidium from southeastern Brazil is described based on morphological and molecular evidence from specimens collected between the rio Jucuruçu and rio Doce basins. The new species belongs to a group of species within Characidium with an unscaled area in the isthmus and is distinguished from these species, except C. alipioi, C. fasciatum, C. hasemani, and C. kamakan, by the greater distance (greater than 10% SL) and presence of 5-7 scales between the anus and the anal fin, and presence of 14 series of scales around the caudal peduncle. The species is distinguished from C. alipioi by having 4 series of scales above the lateral line (vs. 5 series) and greater distance between the anus and the anal fin; from C. fasciatum and C....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bahia; DNA barcode; Espírito Santo; Rapids; South American darter.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000200212
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Morphometric variation of the Herichthys bartoni (Bean, 1892) species group (Teleostei: Cichlidae): How many species comprise H. labridens (Pellegrin, 1903)? Neotropical Ichthyology
Mejía,Omar; Pérez-Miranda,Fabián; León-Romero,Yatzil; Soto-Galera,Eduardo; Luna,Efraín de.
Cichlids of the tribe Heroini have long been a source of taxonomical conflict. In particular, the species included in the Herichthys bartoni group have failed to be recovered as monophyletic in different molecular studies. In this paper we use traditional and geometric morphometrics to evaluate morphological variation in the species included in the H. bartoni complex in order to evaluate the number of species it contains. An update of a previously published DNA barcoding study suggests the existence of three genetic clusters that included the six recognized species analyzed in this study, none of them recovered as monophyletic. On the other hand, geometric morphometrics arise as a useful tool to discriminate species due that traditional morphometrics...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cichlids; COI; DNA barcode; Geometric morphometrics; Mexico.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100061
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