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| Registros recuperados: 60 | |
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| Pérez Carbajal, Carlos. |
| Este experimento se llevó a cabo para analizar los efectos inmunomodulatorios de arginina (ARG) y vitamina E (VE) en pollos de engorda infectados con coccidias. Un total de 300 pollos, vacunados contra coccidiosis (Coccivac®-B) al primer día de edad, fueron divididos en seis grupos con la finalidad de evaluar tres niveles de ARG en el alimento (NARG, 1.44%; MARG, 1.74%; HARG, 2.04%) y dos niveles de VE (40 u 80 UI / kg de alimento, VE40 y VE80, respectivamente) en un diseño factorial 3 x 2. Los pollos se criaron en piso y se les proporcionó agua y una dieta a base de maíz y pasta de soya ad libitum. Al d 14, todos los pollos se infectaron oralmente con un cultivo de diferentes especies del género Eimeria (E. acervulina, E. maxima y E. tenella). A los 9,... |
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Palavras-chave: Pollos de engorda; Arginina; Vitamina E; Respuesta inmunitaria; Broilers; Arginine; Vitamin E; Immune response; Maestría; Ganadería. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/63 |
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| Pérez Carbajal, Carlos. |
| Este experimento se llevó a cabo para analizar los efectos inmunomodulatorios de arginina (ARG) y vitamina E (VE) en pollos de engorda infectados con coccidias. Un total de 300 pollos, vacunados contra coccidiosis (Coccivac®-B) al primer día de edad, fueron divididos en seis grupos con la finalidad de evaluar tres niveles de ARG en el alimento (NARG, 1.44%; MARG, 1.74%; HARG, 2.04%) y dos niveles de VE (40 u 80 UI / kg de alimento, VE40 y VE80, respectivamente) en un diseño factorial 3 x 2. Los pollos se criaron en piso y se les proporcionó agua y una dieta a base de maíz y pasta de soya ad libitum. Al d 14, todos los pollos se infectaron oralmente con un cultivo de diferentes especies del género Eimeria (E. acervulina, E. maxima y E. tenella). A los 9,... |
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Palavras-chave: Pollos de engorda; Arginina; Vitamina E; Respuesta inmunitaria; Broilers; Arginine; Vitamin E; Immune response; Maestría; Ganadería. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/63 |
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| Wang,Mengqiang; Zhang,Daoxiang; Wang,Lingling; Gai,Yunchao; Zhou,Zhi; Zhang,Huan; Song,Linsheng. |
| Background: Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds and interacts with a variety of proteins to regulate their activities. Results: The full-length cDNA of crab Eriocheir sinensis CypA (EsCypA) was cloned by EST and RACE technique. The complete sequence of EsCypA cDNA contained a 5' untranslated region (UTR) of 50 bp, a 3’ UTR of 233 bp with a polyA tail, and an open reading frame (ORF) of 495 bp encoding a polypeptide of 164 amino acids with the predicted molecular weight of 17.36 kDa. The deduced amino acid sequence of EsCypA contained two highly conserved signature sequences of... |
| Tipo: Journal article |
Palavras-chave: Antifungal activity; Cyclophilin A; Eriocheir sinensis; Immune response; PPIase activity. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200002 |
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| Gan,LiNa; Qin,WeiYun; Wu,Sen; Wu,ShengLong; Bao,WenBin. |
| Abstract MYD88 plays an important role in the immune response against infections. To analyze MYD88 gene expression during different stages of pig development, we used real-time PCR. MYD88 was seen expressed in all tissues examined. MYD88 expression in spleen, lungs, and thymus reached its highest value from 7 to 14 days of age and decreased thereafter. Expression in lymph nodes was high until 28 days of age and then it declined after weaning, with stable low levels in adult pigs. MYD88 expression was high before 35 days of age in the small intestine (duodenum, jejunum, and ileum), where it reached its highest value from 7 to 14 days of age. MYD88 expression in the small intestine declined post-weaning and remained relatively low during adulthood. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MYD88; Pig; Developmental expression; Immune response. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100119 |
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| Fonseca,Isabela; Silva,Priscila Vendramini; Lange,Carla Christine; Guimarães,Marta F.M.; Weller,Mayara Morena Del Cambre Amaral; Sousa,Katiene Régia Silva; Lopes,Paulo Sávio; Guimarães,José Domingos; Guimarães,Simone E.F.. |
| In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytokine; Gene expression; Immune response; Real-time PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400018 |
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| Pham, Dominique; Ansquer, Dominique; Chevalier, Anne; Dauga, Clement; Peyramale, Aude; Wabete, Nelly; Labreuche, Yannick. |
| In New Caledonia, shrimp hatcheries are confronted with mass mortality in the larval stages, a phenomenon poorly understood as no specific causative agent has been identified. This has resulted in an excessive use of prophylactic antibiotics, although their adverse effects in aquaculture are notorious. The present work was thus aimed at selecting potential probiotic strains for penaeid hatcheries. From a pool of more than 400 marine bacterial isolates sampled from the local marine environment seven strains exhibited in vitro antagonistic activity towards Vibrio harveyi. These isolates were characterized both phenotypically and genotypically using a biochemical approach and 16S rDNA sequencing. Six out of these seven strains were found to belong to the... |
| Tipo: Text |
Palavras-chave: Shrimp; Litopenaeus stylirostris; Probiotic; Hatchery; Pseudoalteromonas; Immune response. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00190/30129/28591.pdf |
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| De Lorgeril, Julien; Gueguen, Yannick; Goarant, Cyrille; Goyard, Emmanuel; Mugnier, Chantal; Fievet, Julie; Piquemal, D; Bachere, Evelyne. |
| Understanding of antimicrobial defence mechanisms of penaeid shrimp should help in the design of efficient strategies for the management and disease control in aquaculture. In this study, we have specifically analysed the expression in circulating hemocytes of antimicrobial peptides (AMPs) encoding genes, such as PEN2 and PEN3, ALF, crustin, lysozyme and a putative cysteine-rich peptide. We evidenced a relationship between the level of expression of some AMPs and the successful response of the shrimp, Litopenaeus stylirostris, to circumvent a pathogenic Vibrio penaeicida infection. Additionally, significant differences in some AMP transcript amounts are evidenced between control, non-selected shrimp line and the third generation breeding of shrimp selected... |
| Tipo: Text |
Palavras-chave: Real time PCR; Cysteine rich peptide; Crustin; Anti LPS factor; Lysozyme; Penaeidins; Immune response; Vibrio penaeicida; Penaeid; Decapoda; Crustacean. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4524.pdf |
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| Lachambre, Sebastien; Day, Rob; Boudry, Pierre; Huchette, Sylvain; Rio-cabello, Antoine; Fustec, Tirnothee; Roussel, Sabine. |
| Farming, and thus the domestication of Haliotis tuberculata, began recently. We compared the responses of unselected farmed and wild abalone to stressors that occur on farms. The aim was to determine if the farm environment had induced differences in the behavioural or physiological performances of the abalone. Thirty hatchery-born 3.5 year-old abalone and thirty wild ones were reared under standard farm conditions for 6 months and characterised for 19 traits related to growth, survival, behaviour and immunology. Behavioural and immunological responses to stressors differed between the two stocks. Farmed abalone retracted and swivelled less in reaction to a finger contact. Phagocytosis efficiency was reduced by shaking in abalone from both origins, but the... |
| Tipo: Text |
Palavras-chave: Abalone aquaculture; Domestication syndrome; Behavioural adaptation; Responses to stress; Immune response; Farm stressors. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00395/50598/53725.pdf |
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| Smits, M.; Artigaud, Sebastien; Bernay, B.; Pichereau, Vianney; Bargelloni, L.; Paillard, Christine. |
| Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and host-pathogen relationships. It has become increasingly clear that characterizing the functional profiles of response to a disease is an essential step in understanding resistance mechanisms and moving towards more effective disease control. The Manila clam, Ruditapes philippinarum, is a main cultured bivalve species of economic importance which is affected by... |
| Tipo: Text |
Palavras-chave: Bivalve; Diseases; Resistance; Manila clam; Vibrio tapetis; Proteomics; Immune response. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00615/72670/71758.pdf |
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| Fernández-boo, Sergio; Gervais, Ophelie; Prado-alvarez, Maria; Chollet, Bruno; Claverol, Stéphane; Lecadet, Cyrielle; Dubreuil, Christine; Arzul, Isabelle. |
| Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Crassostrea gigas; Ostrea edulis; Flow cytometry; Immune response; Proteome. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00594/70602/68795.pdf |
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| Sitja Bobadilla, A; Pena Llopis, S; Gomez Requeni, P; Medale, Francoise; Kaushik, Sadasivam; Perez Sanchez, J. |
| Partial or total replacement of fish meal by a mixture of plant protein (PP) sources (corn gluten, wheat gluten, extruded peas, rapeseed meal and sweet white lupin) balanced with indispensable amino acids was examined in juvenile gilthead sea bream over the course of a 6-month growth trial. A diet with fish meal (FM) as the sole protein source was compared to diets with 50%, 75% and 100% of replacement (PP50, PP75, and PP100). The possible influence of diets on growth performance, plasma metabolites, gut integrity, liver structure, anti-oxidant and immune status was evaluated. Final body weight was progressively decreased with PP inclusion, but in PP50 and PP75-fed fish, feed efficiency (FE) was significantly improved and specific growth rates remained... |
| Tipo: Text |
Palavras-chave: Gilthead sea bream; Cholesterol; Glutathione; Histopathology; Myeloperoxidase; Complement; Lysozyme; Respiratory burst; Immune response; Growth; Plant proteins; Fish meal. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3595.pdf |
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| Astuya, Allisson; Carrera, Crisleri; Ulloa, Viviana; Aballay, Ambbar; Nunez-acuna, Gustavo; Hegaret, Helene; Gallardo-escarate, Cristian. |
| Saxitoxin (STX) is a neurotoxin produced by dinoflagellates in diverse species, such as Alexandrium spp., and it causes paralytic shellfish poisoning (PSP) in humans after the ingestion of contaminated shellfish. Recent studies have suggested that the immune functions of bivalves could be affected by harmful algae and/or by their toxins. Herein, hemocytes are the main effector cells of the immune cellular response. In this study, we evaluated the response of hemocytes from the mussel Mytilus chilensis to STX exposure in a primary culture. Cell cultures were characterized according to size and complexity, while reactive oxygen species (ROS) production was evaluated using a dichlorofluorescein diacetate (DCFH-DA) assay. Finally, phagocytic activity was... |
| Tipo: Text |
Palavras-chave: Saxitoxin; Hemocytes; Immune response; Paralytic shellfish poisoning; Reactive oxygen species (ROS). |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00278/38954/73180.pdf |
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| De Decker, Sophie; Saulnier, Denis. |
| The understanding of reciprocal interactions between Crassostrea gigas and Vibrio sp., whether these be virulent or avirulent, is vital for the development of methods to improve the health status of cultured oysters. We describe an original non-invasive experimental infection technique using cohabitation, designed to explore these interactions. Using real-time PCR techniques we examined the dynamics of virulent and avirulent Vibrio sp. in oyster hemolymph and tank seawater, and made a parallel study of the expression of four genes involved in oyster immune defense: Cg-BPI, Cg-EcSOD, Cg-IκB, Cg-TIMP. No mortality occurred in control animals, but oysters put in cohabitation for 2–48 h with animals previously infected by two Vibrio pathogens suffered... |
| Tipo: Text |
Palavras-chave: Oyster-Vibrio interactions; Pathogenesis; Immune response; Non-invasive experimental challenge; Real-time PCR. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00025/13604/10681.pdf |
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| Picka,M. C. M.; Calvi,S. A.; Lima,C. R. G.; Santos,I. A. T.; Marcondes-Machado,J.. |
| Acute infection by Toxoplasma gondii leads to suppression of cell-mediated immunity, facilitating chronic infection. One of the causes of immunosuppression is Interleukin-10 (IL-10) production. Glucan is used to stimulate phagocytosis. Our objective was to study IL-10 induction in male BALB/c mice with acute T. gondii BTU-2 strain infection, glucan immunostimulation, and sulfadiazine treatment. Animals were distributed into 7 groups: G1: infected with T. gondii; G2: infected with T. gondii and treated with sulfadiazine; G3: infected with T. gondii and immunostimulated with glucan; G4: infected with T. gondii, immunostimulated with glucan, and treated with sulfadiazine; G5: imunostimulated with glucan; G6: treated with saline; and G7: treated with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Interleukin-10; Glucan; Immune response; Sulfadiazine. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400012 |
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| Gatto,Mariana; Oliveira,Larissa Ragozo Cardoso; Dias,Fernanda De Nuzzi; Araújo Júnior,João Pessoa; Lima,Carlos Roberto Gonçalves; Lordelo,Eliana Peresi; Santos,Rodrigo Mattos dos; Kurokawa,Cilmery Suemi. |
| Abstract Background The present study evaluated the effect of treatment with benznidazole on mRNA expression of IFN-γ, IL-17, IL-10, TGF-β and FoxP3 in spleen and heart tissue of BALB/c mice in the acute phase of an experimental infection with Trypanosoma cruzi, strains JLP or Y. Methods The mRNA expression of cytokines and parasite load were assessed by q-PCR. Dependent groups were compared using Student's paired t-test and independent groups were compared using Student's unpaired t-test. Results Infection with the JLP or Y strains increased expression of IFN-γ in the heart and of IL-10 and IL-17 in the spleen and heart compared to uninfected animals. Treatment increased the expression of IFN-γ and decreased the expression of IL-17, IL-10, TGF- β and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chagas disease; Trypanosoma cruzi; Immune response; Cytokines; Q-PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100320 |
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| Registros recuperados: 60 | |
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