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| Registros recuperados: 97 | |
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| Martínez Hernández, María de Jesús. |
| La multiplicación de plantas in vitro presenta varias etapas críticas, desde la selección de los componentes para la germinación, multiplicación y enraizamiento hasta el proceso de aclimatación en invernadero. En la presente investigación se utilizaron los sustratos inertes (vermiculita, agrolita y tezontle) como medio de soporte en sustitución del agar y los azúcares xilosa, fructosa y 10X20F se compararon con la sacarosa, en su efecto sobre la germinación, multiplicación, enraizamiento y sobrevivencia de los portainjertos de cítricos: Citrus volkameriana, Citrumelo swingle y Citrange 35. Además de probar diferentes intensidades luz en el proceso de aclimatación de plántulas de Citrus volkameriana con diferentes sustratos y una reducción gradual... |
| Tipo: Tesis |
Palavras-chave: Cultivo in vitro; Sustratos inertes; Cítricos; Doctorado; Agroecosistemas tropicales; Tissue culture; Inert substrates; Citrus. |
| Ano: 2007 |
URL: http://hdl.handle.net/10521/1263 |
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| Ayala-Astorga,Gloria Irma; Alcaraz-Meléndez,Lilia. |
| We evaluated the effects of saline stress on soluble proteins, lipid peroxidation (TBAR), chlorophyll a, chlorophyll b, β-carotene, violaxanthin, and proline in Paulownia imperialis and Paulownia fortunei plants grown in vitro. When the propagated plants reached a determined size, they were transferred aseptically to WPM culture medium containing different sodium chloride concentrations (0, 20, 40, 60, 80, and 160 mM) and were sampled at 15 and 30 days. Proline content was determined at 30 days after transfer only. Protein concentration significantly decreased with the highest salt levels in P. imperialis compared to controls in which no sodium chloride was added. In both P. imperialis and P. fortunei, lipid peroxidation significantly increased at... |
| Tipo: Journal article |
Palavras-chave: Paulownia fortunei; Paulownia imperialis; Pigments; Proline; Proteins; Salinity; TBARS; Tissue culture. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500013 |
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| Demirkiran,Aykut; Marakli,Sevgi; Temel,Aslihan; Gozukirmizi,Nermin. |
| Morphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreased fresh weight and protein content, and increased SOD (EC 1.15.1.1) activity in a dose-dependent manner. The lower concentration increased root growth. Salinity caused nucleotide variations in roots, but did not affect shoot DNAs. The higher concentration caused methylation changes, mainly hypermethylation in shoots. This is the first study on genetic and epigenetic effects of salinity in barley. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hordeum vulgare; Salt stress; Tissue culture; RAPD; CRED-RA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400016 |
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| Santa-Rosa,Sandra; Souza,Fernanda VD; Vidal,Ádila M; Ledo,Carlos Alberto da S; Santana,José RF de. |
| Aechmea blanchetiana and Aechmea distichantha are Brazilian native bromeliads with great ornamental value, although none of the species are produced commercially. Therefore, market demands are met through nature predatory exploitation. This study intended to (1) evaluate the in vitro seed germination of A. blanchetiana and A. distichantha and (2) establish micropropagation protocols for both species, aiming at producing plantlets for the market, as well as to germplasm in vitro conservation. Germination experiments were carried out in completely randomized designs with two treatments (presence and absence of light) and seven and eight replications respectively for A. blanchetiana and A. distichantha. In vitro seedlings, produced under light conditions,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bromeliaceae; Tissue culture; In vitro multiplication rate; Growth regulators. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000100018 |
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| Scotton,Danielle C; Benedito,Vagner Augusto; Molfetta,Jeanne B de; Rodrigues,Benedita Inês FP; Tulmann-Neto,Augusto; Figueira,Antonio. |
| Garlic cultivars are sexually sterile under standard growth conditions, with direct implications for commercial production costs as well as breeding programs. Garlic is propagated commercially via bulblets, which facilitates disease transmission and virus load accumulation over vegetative generations. Tissue culture produces virus-free clones that are more productive, while keeping the desired traits of the cultivar. Consequently, this technique allows studies of garlic genetics as well as guarantees genetic conservation of varieties. We aimed at analyzing the in vitro regeneration of eight marketable cultivars of garlic using root segments as explants. For each genotype, bulblet-derived explants were isolated and introduced into MS medium supplemented... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium sativum; Bulb; Callus induction; Organogenesis; Regeneration; Tissue culture. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000100013 |
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| Cardoso,Jean C; Ono,Elizabeth O. |
| One of the most important applications of plant tissue culture is mass propagation of ornamental plants. This experiment evaluated the effect of different concentrations of NH4NO3 and KNO3 and BAP on the in vitro growth of orchid hybrid Brassocattleya 'Pastoral'. Seedlings of this orchid hybrid were used as explants and cultivated in medium with mineral salts and vitamins from the MS medium (Murashige & Skoog, 1962), with the macronutrients P, Ca and Mg reduced by half, and with an addition of 25 g L-1 of sucrose, 0.1 g L-1 of myo-inositol and 1.5 g L-1 of activated charcoal. Agar-agar was added (6.5 g L-1) and the pH was adjusted to 5.8. As treatments, four concentrations of the NH4NO3 and KNO3 (2x; 1x; ½ and ¼ MS medium) and three concentrations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Macronutrients; Cytokinins; Multiplication; Growth. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000300017 |
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| RIBEIRO, J. M.; MELO, N. F. de; OLIVEIRA, V. R. de. |
| O objetivo deste trabalho foi estabelecer e multiplicar, sob condições de cultivo in vitro, um acesso de faveleira sem espinhos, visando alternativas para sua conservação e uso futuro. Para isso, segmentos nodais e fragmentos de folhas jovens foram inseridos em meio nutritivo composto pela formulação de sais inorgânicos WPM. Para os segmentos nodais, o meio de cultura foi suplementado com as seguintes concentrações (mg/L) de BAP (6-benzilaminopurina): T1:0; T2:0,1; T3:0,5; T4:1,0 e T5:1,5. Já aquele em que foram utilizados fragmentos de folhas jovens como explantes, foram testadas as seguintes concentrações (mg/L) de 2,4-D (ácido 2,4-diclorofeno-xiacético): T1:0; T2:10; T3:20, T4:40 e T5:80. Decorridos 30 e 60 dias, foram tomados dados relacionados ao... |
| Tipo: Folhetos |
Palavras-chave: Favela; Faveleira sem espinhos; Planta nativa; Espécie forrageira; Manutenção de germoplasma; Contaminação in vitro; Oxidação in vitro; Cultivo in vitro; Selo ODS 2; Bioma Caatinga; Caatinga; Propagação Vegetativa; Cultura de Tecido; Biodiversidade; Tecido Vegetal; Espécie Nativa; Cnidoscolus quercifolius; Vegetative propagation; Tissue culture. |
| Ano: 2022 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1147865 |
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| OLIVEIRA, J. R. P.; SOARES FILHO, W. dos S.; NASCIMENTO, A. S. do; Costa, D. da C.; MATSUURA, F. C. A. U.; GOMES, J. de C.; CARVALHO, J. E. B. de; REINHARDT, D. H. R. C.; OLIVEIRA, R. P. de. |
| Por sua riqueza em vitamina C e adaptação e ambientes tropicais, a cultura da acerola tem se expandido em varias regiões do pais. Os pomares comerciais brasileiros, porem, apresentam grande desuniformidade entre plantas, no tocante a diversos caracteres de importância agronômica, a exemplo de: produtividade, qualidade de frutos, conformação e vigor da copa. Essa variabilidade genética, todavia, enriquecida pela introdução de variedades promissoras provenientes do exterior, particularmente de Porto Rico e Estados Unidos da América do Norte (Florida e Havai), pode permitir a seleção de genótipos horticulturalmente superiores, possíveis de serem indicados como cultivares adaptadas aos nossos diferentes agro ecossistemas. Com base no exposto, a Embrapa... |
| Tipo: Documentos (INFOTECA-E) |
Palavras-chave: Selecao de genotipos; Manejo cultural; Cultura de tecidos; Genotype selection; Cultural management.; Malpighia Punicifolia.; Tissue culture; Vegetative propagation.. |
| Ano: 1998 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/638286 |
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| Registros recuperados: 97 | |
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