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DDS技術を活用した獣医再生治療 OAK
佐々木, 直樹.
Recently, regenerative medicine with autologous cells and tissues has been carried out for tissue regeneration. Basic research and clinical application based on tissue engineering have also been reported in joint cartilage regeneration. The physiological connection between the cartilages newly regenerated and natural ones is not always sufficient. Currently, to tackle the problem, the clinical application of DDS-based cartilage regeneration therapy has been tried. This paper introduces the research results of bone regeneration and articular cartilage regeneration in the veterinary medical field. c 2017, Japan Society of Drug Delivery System. All rights reserved.
Palavras-chave: Basic fibroblast factor; Bone morphogenetic protein; Platelet rich plasma; Transforming growth factor-β1; Β-tricalcium phosphate.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4543
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A Mechanism of Excessive Accumulation of Abomasal Gas in Vagotomized Cattle Determined using Fluoroscopy OAK
ITOH, Megumi; SASAKI, Naoki; KAWAMOTO, Satoshi; YAMADA, Haruo; INOKUMA, Hisashi; 佐々木, 直樹; 猪熊, 壽.
To better understand the mechanism of excessive gas accumulation in the abomasum in bovine abomasal displacement, we performed gastric fluoroscopy in vagotomized cattle. Fifteen 6-month-old Holstein steers were divided into three groups: a non-vagotomized control group (Group C; n=5), a ventral thoraco-vagotomized group (Group V; n=5), and a dorsal and ventral thoraco-vagotomized group (Group DV; n=5). These groups were examined by fluoroscopy before and during a 5-week observation period after surgery. In Group C, no change was observed throughout the observation period. In Group DV, immediately after surgery, reticuloruminal motility was completely absent and ruminal distention was seen. Two weeks after surgery, abnormal reticulum motility and increased...
Palavras-chave: Abomasum gas; Cattle; Displaced abomasum; Vagotomy.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3888
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Use of Contrast-Enhanced CT in the Diagnosis of Abscesses in Cattle OAK
LEE, Ki-Ja; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; SASAKI, Naoki; ISHII, Mitsuo; INOKUMA, Hisashi; IWASAKI, Toshiroh; MIYAKE, Yoh-Ichi; YAMADA, Kazutaka; 古林, 与志安; 松本, 高太郎; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽; 山田, 一孝.
We report here the non-contrast and contrast-enhanced computed tomography performed in two calves with brain abscess and multiple pulmonary abscesses with pharyngeal abscess, respectively. Contrast-enhanced computed tomography was useful in the diagnosis of these diseases in both calves. The diseases were confirmed by histopathological examination.
Palavras-chave: Abscess; Cattle; Contrast-enhanced CT; Diagnosis.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3891
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オーストラリア・クイーンズランド大学における産業動物臨床教育 : 教育GP海外視察報告 OAK
山田, 一孝; 石井, 三都夫; 佐々木, 直樹; 古林, 与志安.
http://nichiju.lin.gr.jp/mag/06209/index.html
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2808
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ペルシュロン種繁殖雌馬にみられた遠隔転移を伴う腎細胞癌の1症例 OAK
久保田, 直樹; 村上, 智亮; 安田, 峰; 松井, 基純; 古林, 与志安; 古岡, 秀文; 松井, 高峯; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽.
19歳齢のペルシュロン種繁殖雌馬に血尿が認められた。血尿は止血剤および抗生剤投与によっては改善され なかった。患畜は次第に元気食欲の低下、削痩、貧血を呈し、高度蛋白尿および胸前部冷性浮腫が発現した。 直腸検査により人頭大に腫大した左腎を触知し、腎臓腫瘍を疑った。病理解剖の結果、左腎は27X18Xl0cmに 腫大し、血液に富む巨大な腫瘤形成(13X8X8cm)を認めた。左腎門リンパ節は腫大し、また右腎臓、左右 副腎、肝臓および肺では大小の白色腫瘤を認めた。病理組織学的検査によりこれらの腫瘍はいずれも浸潤性に 増殖する上皮性腫瘍組織であったことから、本症例は腎細胞癌が他の複数の臓器に転移したものと考察した。
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2935
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日本ウマ科学会第7回馬臨床獣医師ワーキンググループ招待講演・症例検討会・実習について OAK
樋口, 徹; 佐々木, 直樹.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4025
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馬におけるフェニルブタゾンとシクロオキシゲナーゼ-2 : 選択阻害薬投与による胃粘膜への影響 OAK
山家, 崇史; 都築, 直; 徐, 鍾筆; 大下, のえ; 中尾, 奨吾; 田邉, 貴史; 佐々木, 直樹; SASAKI, Naoki.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3887
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Effects of Gelatin Hydrogel Microsphere Containing Platelet Rich Plasma Dairy Cows with Sole Ulcers OAK
内山, 裕貴; 都築, 直; 徐, 鍾筆; 山田, 一孝; 羽田, 真悟; 眞鍋, 弘行; 田畑, 泰彦; 佐々木, 直樹.
自家血液から作製された多血小板血漿(Platelet rich plasma,以下PRP)は,アルギン酸ゲル剤として患部に塗布することで蹄底潰瘍への良好な治癒効果が認められた.しかし,効果を持続させるという点で不十分な方法であった.そこで本研究では,徐放剤としてゼラチンマイクロスフィア(以下 GM)を用いた新しい塗布剤を作製し,治療効果を検討した.PRP含浸GM混合アルギン酸ゲル処置群(PRP群,n=10)とアルギン酸ゲルのみ処置群 (対照群,n=9)を比較した.蹄底潰瘍面積の欠損割合は,PRP群で処置後1,2及び3週目で約45%,17%及び5%と推移し,対照群と比較して有意な角質再生効果がみられた.また,PRP群では処置後2週目に圧痛が消失したが,対照群では痛みは継続していた.以上のことから,PRP含浸GM混合アルギン酸ゲルは乳牛の蹄底潰瘍に有効であることが明らかとなった.
Palavras-chave: ゼラチンマイクロスフィア; 多血小板血漿; 蹄底潰瘍.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3905
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ヨーロッパ獣医科大学における臨床獣医学教育の現状 OAK
佐々木, 直樹; 山田, 一孝; 石井, 三都夫; 猪熊, 壽.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3885
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馬の消化器病の診断法 OAK
佐々木, 直樹.
Palavras-chave: ; 急性腹症; 消化器病診断.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/893
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2013年度アメリカ獣医外科学会(ACVS) OAK
佐々木, 直樹.
海外報告
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4020
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喉頭粘膜下膿瘍による顕著な喘鳴を呈したホルスタイン種乳牛の1症例 OAK
田川, 道人; 神尾, 恭平; 大林, 哲; 佐々木, 直樹; 古岡, 秀文; 猪熊, 壽.
3歳2カ月齢のホルスタイン種乳牛が発熱、頻脈および喘鳴を呈した。症状は抗生剤とステロイド投与によ りいったん改善されたが、その後再発し治療に反応しなくなった。内視鏡検査により咽喉頭部の腫大が認められた。病理解剖により喉頭粘膜下膿瘍と診断され、膿瘍からはArcanobacterium pyogenseが分離された。
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2936
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MDCT Images of the Head of a Horse with Malignant Melanoma OAK
SASAKI, Naoki; MINAMI, Takuto; YAMADA, Kazutaka; SATOH, Motoyoshi; INOKUMA, Hisashi; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; YAMADA, Haruo; 佐々木, 直樹; 山田, 一孝; 猪熊, 壽; 古林, 与志安; 古岡, 秀文.
A 17-year-old, gray Thoroughbred gelding was presented with recurrent epistaxis following exercise for over one year. Multidetector-row computed tomographic (MDCT) examination of the head performed under general anesthesia indicated the presence of malignant melanoma. MDCT examination revealed the exact location and size of masses in the right guttural pouch first identified by endoscopy and in the left guttural pouch unidentified by endoscopy. MDCT examination revealed the presence of a blood clot that filled the right maxillary sinus. The horse was euthanized due to poor prognosis as a riding horse and the owner's decision, and MDCT findings were confirmed by postmortem examination. MDCT is suggested as a useful technique for diagnosis of head problems...
Palavras-chave: Equine; Malignant melanoma; MDCT.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3104
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重種子馬の総指伸筋腱断裂を疑った1 例 OAK
佐々木, 直樹; 高桑, 潤; 関塚, 次郎; 山田, 明夫; SASAKI, Naoki; TAKAKUWA, Jun; SEKIZUKA, Jirou; YAMADA, Haruo.
北海道十勝地区で出生した重種子馬に,出生直後より起立が不自由であり,左側腕節外側面に直径3cm の腫脹が認められた.生後15 日目の超音波検査において,左側腕節前面の腱実質が確認されず,片側性総指伸筋腱断裂と診断された.圧定包帯によって歩様は改善し,最終的に生後270 日目に市場取引された.本症例はわが国で最初の報告例である. In a draught foal born in Tokachi,Hokkaido,Japan,dysstasia and a cold tumor of 3cm diameter were observed in the lateral surface of the left carpal region immediately after birth. At Obihiro University,an ultrasonic examination revealed an absence of the tendon parenchyma on the anterior surface of the left carpal region,and a diagnosis of congenital rupture of the common digital extensor tendons was suggested. Compression bandages improved the gait,and eventually the foal was traded when it was 270 days old.
Palavras-chave: 総指伸筋腱断裂; ; 超音波診断; Common digital extensor tendons; Equine; Diagnostic ultrasound.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2806
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Medicinal Treatment to Equine Gastrointestinal Dysfunctions OAK
SASAKI, Naoki; YAMADA, Haruo; HARA, Shigeo; 佐々木, 直樹.
This study was designed to investigate gastrointestinal dysfunctions, especially the effects of gastrointestinal prokinetic agents on digestive disorders involving lowered motility. The effects of α2-adrenergic agonists for treating acute abdominal disorders involving intestinal torsion in horses were also investigated. The results are as follows: (1) It appeared that the functions of migrating contractions (MC) in horses were to transfer the contents of the digestive tract as soon as possible to the cecum and colon, in addition to maintaining homeostatis in the digestive tract. (2) Motilin was seen to be involved in the regulation mechanism of intestinal motility in the horse as well as in humans and dogs, and brought about MC. (3) The intravenous...
Palavras-chave: Α2-adrenergic agonist; Equine; Gastrointestinal dysfunction; Motility; Serotonin receptor agonist.
Ano: 2003 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3099
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ポータブルX線撮影装置を用いた牛の Computed Radiography 画像化因子が画質に与える影響 OAK
岸本, 海織; 住谷, 峻; 李, 奇子; 清水, 純一郎; 山川, 和宏; 滄木, 孝弘; 羽田, 真悟; 松本, 高太郎; 石井, 三都夫; 佐々木, 直樹; 猪熊, 壽; 山田, 一孝; KISHIMOTO, Miori; SUMIYA, Takashi; LEE, Ki-Ja; SHIMIZU, Junichiro; YAMAKAWA, Kazuhiro; AOKI, Takahiro; HANEDA, Shingo; MATSUMOTO, Kotaro; ISHII, Mitsuo; SASAKI, Naoki; INOKUMA, Hisashi; YAMADA, Kazutaka.
牛臨床におけるComputed Radiography (CR) の有用性を確立するため,管球-カセッテ間距離およびImaging Plate (IP) の読み取り時間が画質に与える影響について検討した.管球-カセッテ間距離は画質の視覚的スコア(粒状性,鮮鋭性)に影響しなかった. IPの読み取り時間は画質の鮮鋭性と相関しなかったが,粒状性と相関が認められた.しかし読み取り時聞が12時間以内の場合には問題にならない程度であると考えられた.管球-力セッテ間距離を再現よく保つことが難しく, IP読み取り時間のばらつきが想定される牛の臨床画像診断においてCRは有用なX線撮影システムであると考えられた. The purpose of this study was to investigate the effect of X-ray tube-cassette distance and image scanning time on image quality to establish the benefit of Computed Radiography (CR) in bovine clinical practice. The tube-cassette distance had no effect on the visual evaluation score (graininess and sharpness). The image scanning time correlated with graininess but not sharpness. From these results,it was concluded that accidental variations in the tube-cassette...
Palavras-chave: ; Computed Radiography (CR); フェーデイング; Imaging Plate (IP); ポータブルX線発生装置; Bovine; Computed Radiography (CR); Fading; Imaging Plate (lP); Portable x-ray generator.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2939
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Effects of Orally Administrated Mosapride, a 5-HT4 Receptor Agonist on the Digestive Tract of Horses OAK
SASAKI, Naoki; LEE, Inhyung; AYUKAWA, Yu; YAMADA, Haruo; 佐々木, 直樹.
The effects of mosapride, a 5-hydroxytryptamine 4 (5-HT4) receptor agonist, on the motility of horse small intestine were examined by means of electrointestinography (EIG). Six adult healthy thoroughbreds were used, in which EIG signals from the small intestine were measured by a Degetorappar EGG system. Mosapride, dissolved in 200 ml water, was orally administered at a dose of 2 mg/kg. The total power of EIG signals at a frequency band of 3 cpm was 419.1 ± 122.0 (μV) 2 × cpm (n=6) before, and 2,093.6 ± 850.0 (μV)2 × cpm (n=6) after the drug administration, the latter mean value being significantly greater than the former. This result suggests that mosapride has a prokinetic action on the motility of horse small intestine.
Palavras-chave: Equine; Gastrokinetic agent; Mosapride; Oral administration; 5-HT4 receptor agonist.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3100
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ウマの胃排出能に対するクエン酸モサプリドの促進効果(内科学) OAK
OKAMURA, Koichi; SASAKI, Naoki; FUKUNAKA, Morito; YAMADA, Haruo; INOKUMA, Hisashi; 佐々木, 直樹; 猪熊, 壽.
The prokinetic effect of the 5-HT4 receptor agonist mosapride was evaluated in seven healthy thoroughbreds. Mosapride was orally administerd at doses of 0.5, 1.0 or 1.5 mg/kg. The breath 13CO2/12CO2 rate (Δ13CO2), an indirect indicator for the rate of gastric empting, was measured at appropriate points for 4 hr after drug administration. There was a significant increase compared with the control value at 15, 20 and 165 min for 0.5 mg/kg, 30 min at 1.0 mg/kg and 165 min for 1.5 mg/kg. The results suggest that mosapride may facilitate the gastric emptying in horses.
Palavras-chave: Equine; Gastric emptying rate; Mosapride citrate.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4019
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Effect of Compound of Gelatin Hydrogel Microsphere Incorporated with Platelet-Rich-Plasma and Alginate on Sole Defect in Cattle OAK
TSUZUKI, Nao; SEO, Jong-Pil; YAMADA, Kazutaka; HANEDA, Shingo; TABATA, Yasuhiko; SASAKI, Naoki; 羽田, 真悟; 佐々木, 直樹.
Platelet-rich plasma (PRP) was administered to cattle with hoof defects (10-mm drill hole) to promote sole defect healing. Two types of gels were prepared. One was a mixed gel [compound of gelatin microspheres (GM) incorporated with PRP and alginate] and was used for the test group, while the other was an alginate gel used for the control group. Each hole was filled with one of these gels. The drill hole depth, pressure pain, and hardness of the regenerated tissue were measured for 3 weeks after treatment. These measurements improved significantly in the test group than in the control group. The results suggest that a compound of GM incorporated with PRP and alginate had a hoof-regenerating effect in cattle.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3889
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十勝地区における馬の疝痛発症状況 OAK
上林, 義範; 都築, 直; 徐, 鍾筆; 大下, のえ; 中尾, 奨吾; 田邊, 貴史; 山家, 崇史; 内山, 裕貴; 佐々木, 直樹; SASAKI, Naoki.
十勝地区において疝痛を発症した馬36頭を対象として、疝痛発症の危険因子および疝痛を発症した馬の予後 に関する疫学調査を実施した。調査の結果から、十勝地区における馬の疝痛発症には、過去の疝痛既往歴、駆 虫剤投与歴、飼養管理の急変などが関連していることが推測された。疝痛の種類別に来院時のヘマトクリット 値を比較すると、便秘疝の馬では変位疝や風気疝の馬と比較して有意に高値を示した。手術実施馬において、 疝痛発症から来院までの時間が長いほど予後が悪化する傾向がみられた。また、予後不良となった馬では術後 経過が良好で退院した馬と比較して、心拍数が有意に高値を示すということが判明した。
Palavras-chave: ; 疝痛; 調査.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4023
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