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| Registros recuperados: 26 | |
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| YAMADA, Kazutaka; TANABE, Shinobu; UENO, Hiroshi; OINUMA, Akemi; TAKAHASHI, Toyomi; MIYAUCHI, Satoshi; SHIGENO, Satoshi; HIROSE, Tsuneo; MIYAHARA, Kazuro; SATO, Motoyoshi; 山田, 一孝; 宮原, 和郎. |
| Chondroitinase ABC (C-ABC) is expected to be a novel agent for chemonucleolysis. The effect of C-ABC was investigated by magnetic resonance (MR) and radiograph. C-ABC was administered into the lumbar intervertebral disks on the clinically normal beagles (n=5), in a dose of 50 μl (12.5 units as C-ABC). MR scans were performed pre-dose, and 1, 3, 7, 14 and 28 days after administration of C-ABC, and the signal intensity (SI) of the nucleus pulposus was measured. Radiographs were taken pre-dose, and 1, 2, 3, 4, 5, 6, 7, 14 and 28 days post-dose, to evaluate narrowing of the disk space in terms of height index (HI). In addition, the quantity of the chondroitin sulfate (CS) and the hyaluronic acid (HA) in the nucleus pulposus were measured by high performance... |
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Palavras-chave: Canine; Chemonucleolysis; Chondroitinase ABC (C-ABC); Height index (HI); Magnetic resonance (MR). |
| Ano: 2001 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4181 |
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| TAKAHASI, Shoko; MIYAHARA, Kazuro; ISHIKAWA, Hirosi; ISHIGURO, Naotaka; SUZUKI, Mitsuyoshi; 宮原, 和郎; 鈴木, 三義. |
| To estimate the maternal effects of dog breeds using mitochondrial DNA(mtDNA) haplotypes in the dogs with several clinical disorders, 600 base pairs of mtDNA D-loop region were amplified from 365 dogs and were determined for mtDNA sequences. The diversity of the 600-bp sequences was classified into 64 haplotypes, including 46 newly discovered haplotypes, and the haplotypes were grouped into four clusters I to IV. Lineage analysis using the mtDNA haplotype indicated that each dog breed genetically comprises one or a few mtDNA haplotypes. When the relationship between genetic background and occurrences of clinical diseases was estimated, canine lineage analysis using mtDNA haplotype revealed that the disorders distributed in the dominant mtDNA haplotypes of... |
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Palavras-chave: D-loop; Haplotype lineage analysis; Mitochondrial DNA. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4066 |
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| MIYAHARA, Kazuro; TOSE, Shinichi; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男. |
| In order to identify mechanisms responsible for the anti-tumor effects of Toxoplasma lysate antigen (TLA), we used an in vitro 51Cr release assay to study the functional properties of plastic-adherent cells during induction of splenic cytotoxic activity by TLA. Cytotoxic activity of non-adherent cells was measured in all experiments after a 6 days incubation. Induction of cytotoxic non-adherent cells by TLA required the presence of plastic-adherent spleen cells. In contrast, rhIL-2 alone was able to induce transformation of cytotoxic non-adherent cells from non-adherent spleen cells. Contact between adherent and non-adherent spleen cells was necessary for successful induction of cytotoxic non-adherent cells by TLA. Treatment of spleen cells with... |
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Palavras-chave: Adherent cell; Cytotoxic activity; Macrophage; Spleen cell; Toxoplasma lysate antigen. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4065 |
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| MIYAHARA, Kazuro; MATSUMOTO, Toru; TOSE, Shinichi; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男. |
| Spleen cells from Toxoplasma lysate antigen (TLA)-sensitized BALB/c mice showed the strong cytotoxic activity against both natural killer (NK)-sensitive cells (YAC-1 and RL♂-1) and NK-insensitive cells (P-815), when incubated with TLA or recombinant human IL-2 (rhIL-2). The increment of TLA concentration in culture medium increased the cytotoxic activity. Treatment of effector cells; spleen cells from TLA-sensitized mice incubated with TLA, with anti-asialo GM1 or anti-Thy-1 plus complement inhibited the cytotoxic activity of effector cells, whereas treatment with anti-mouse Lyt-2.2 serum plus complement had no effect on the cytotoxic activity. Treatment of spleen cells from TLA-sensitized mice with anti-asialo GM1 and/or anti-Thy-1 plus complement... |
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Palavras-chave: Cytotoxic activity; Mouse; Phenotype; Spleen cell; Toxoplasma lysate antigen. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4063 |
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| ISHIJIMA, Takako; KOBAYASHI, Yoshiyasu; LEE, Dong-Soo; UETA, Yoshiko Yanagimoto; MATSUI, Motozumi; LEE, Jung-Youn; SUWA, Yoshinori; MIYAHARA, Kazuro; SUZUKI, Hiroshi; 松井, 基純; 宮原, 和郎; 鈴木, 宏志; 古林, 与志安. |
| The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of vitrified-warmed canine ovaries by means of transplantation into non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mice. All ovarian tissues cryopreserved by vitrification were morphologically normal in terms of histology. Cryopreserved ovaries were transplanted into the ovarian bursa of the NOD-SCID mice, and the xenografts were recovered from 23 of 23... |
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Palavras-chave: Canine; Ovary transfer; Transplantation; Vitrification; Xenograft. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3023 |
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| NISHIMURA, Maki; YOSHIDA, Tetsuya; EL-KHODERY, Sabry; MIYOSHI, Masafumi; FURUOKA, Hidefumi; YASUDA, Jun; MIYAHARA, Kazuro; 古岡, 秀文; 宮原, 和郎. |
| To obtain B-mode ultrasound images of mammary glands in dairy heifers at different stages of growth, 25 clinically normal Holstein heifers were used. The heifers were divided into 5 groups (n=5/group) by stage of their growth: 2-month-old (group 1), 5-month-old (group 2), postpuberty (group 3), mid (group 4), and late (group 5) pregnancy. Furthermore, the sections of mammary glands were observed grossly at postmortem examination in one heifer in each group. Ultrasound images varied with the development of mammary glands. In group 1, the mammary glands had distinctive ultrasonographic findings: an oval to fusiform homogeneous hypoechoic structure. In all groups except group 1, mammary tissue consists of two major areas: a homogeneous, medium echogenic area... |
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Palavras-chave: Dairy heifer; Mammary gland; Ultrasound. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4068 |
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| TOMIHARI, Mizuki; NISHIHARA, Akira; SHIMADA, Terumasa; YANAGAWA, Masashi; MIYOSHI, Masafumi; MIYAHARA, Kazurou; OISHI, Akihiro; 富張, 瑞樹; 宮原, 和郎; 大西, 明弘. |
| Most anesthetics have an immuno-suppressive effect on cellular and neurohumoral immunity, and research shows that total intravenous anesthesia (TIVA) with propofol has a greater immuno-protective effect than inhalational anesthesia in human medicine. However, in veterinary clinics, these effects remain ambiguous. To clarify the details, we focused on propofol and isoflurane, investigating clinical blood hematology and immunological profiles drawn from healthy dogs under and after two anesthesia techniques. Twelve healthy adult beagles were included in this study, randomly assigned to the propofol anesthesia group (group P: n=6) or the isoflurane anesthesia group (group I: n=6). In both groups, the number of lymphocytes in peripheral blood decreased after 2... |
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Palavras-chave: Anesthesia; Canine; Immuno-suppression; Isoflurane; Propofol. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4209 |
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| 三好, 雅史; 西村, 麻紀; 浅井, 寛之; 宮原, 和郎; MIYOSHI, Masafumi; NISHIMURA, Maki; ASAI, Hiroshi; MIYAHARA, Kazuro. |
| 三次元超音波検査法の小動物臨床への応用の可能性を探求するために，妊娠後期の6 歳 のビーグル犬に対して，腹部用三次元プローブを用いた三次元超音波検査法を出産まで数 日毎に行い，8 頭の胎子の顔を描出しようと試みた。胎子の顔の三次元超音波画像が全ての 検査日において少なくとも1 頭の胎子で得られたが，胎子が成長するにつれて良好な画像 を得るのは難しくなった。交配後43 日目ならびに45 日目では胎子の顔の鮮明な三次元画 像が容易に得られた。技術的に解決しなければならない問題がいくつか残されているもの の，適切に用いさえすれば，三次元超音波検査法は従来の二次元超音波検査法を補足する 方法として小動物臨床に応用可能であると考えられる。 This study was delineated to explore the possibility of using three-dimensional ultrasonography in small animal practice. In a 6-year-old pregnant beagle bitch with eight feti, we tried to visualize the feti faces with three-dimensional ultrasonography by scanning with a transabdominal three-dimensional probe every two to three days until parturition. At each date of examination, Three-dimensional sonographic images of the feti faces were... |
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Palavras-chave: 犬; 顔; 胎子; 三次元超音波検査法; Dog; Face; Fetus; Three-dimensional ultrasonography. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2834 |
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| Registros recuperados: 26 | |
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