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Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla Scientia Agricola
Alcantara,Giovana Bomfim de; Bespalhok Filho,João Carlos; Quoirin,Marguerite.
The hybrid Eucalyptus grandis x Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E. urophylla was studied in order to verify the effect of several factors: subculture duration on multiplication medium, type of explants, entire and half leaves: basal and apical portions, and duration of the culture on a regeneration medium. Differences in organogenic capacity of the two clones tested were observed. The explant most recommended for organogenesis is the basal section of the leaf collected from shoot clusters subcultured every 17 days. Moreover, the leaf explants must be transferred to a fresh bud induction medium...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Agrobacterium tumefaciens; Micropropagation; Tissue culture; Transgenic plant; Woody species.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000200016
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Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars Ciência Rural
Dibax,Roberson; Alcantara,Giovana Bomfim de; Machado,Marília Pereira; Filho,João Carlos Bespalhok; Oliveira,Ricardo Augusto de.
The objectives of this study were to establish appropriate conditions for obtaining plant regeneration and acclimatization of the 'RB92579' and 'RB93509' sugarcane cultivars and to elucidate the shoots origin through histological analysis. For both cultivars, obtaining shoots showed better results with the culture of explants on a callus induction medium containing 2.0mg L-1 2,4-dichlorophenoxyacetic acid, followed by cultivation on a shoot induction medium containing 0.1mg L-1 kinetin and 0.2mg L-1 benzilaminopurine. The MS medium without growth regulators proved to be appropriate for elongation and rooting of shoots and the use of the composed substrate of vermiculite + MS salts was effective for acclimatization. Histological analysis revealed that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2; 4-dichlorophenoxyacetic acid organogenesis tissue culture acclimatization Poaceae.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100009
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