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Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique Acta Botanica
Padilha,João Henrique Delfrate; Ribas,Luciana Lopes Fortes; Amano,Érika; Quoirin,Marguerite.
Considering the necessity of Acrocomia aculeata propagation for large-scale production, the aim of this study was to establish a somatic embryogenesis protocol using the thin cell layer (TCL) technique. Aerial parts of in vitro plants were transversally cut at the base into eight TCLs and placed in a culture medium for callus induction. The induction medium was composed of Y3 salts and Morel´s vitamins and supplemented with 150, 300 or 600 μM picloram. After 12 weeks the calli were transferred to a medium supplemented with BAP or 2-iP (12.5 or 25 μM). After 18 weeks, the somatic embryo clusters were transferred to a conversion medium (plant growth regulator-free medium). Primary callus induction rate was higher in the first three TCLs and in media...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-iP; Callus induction; Histology; Macaw palm; Picloram.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400516
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