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	Provedor de dados Scientia Agricola (1) Genet. Mol. Biol. (1) Autor Azevedo,Andréia Cristiane Souza (2) Fungaro,Maria Helena Pelegrinelli (2) Sosa-Gómez,Daniel Ricardo (2) Faria,Marcos Rodrigues (1) Furlaneto,Márcia Cristina (1) Palavra-chave Bemisia (1) Biological control (1) Entomopathogenic fungus (1) Paecilomyces fumosoroseus (1) RAPD (1) Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2000 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Effects of double-stranded RNA on virulence of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against the silverleaf whitefly, Bemisia tabaci strain B (Homoptera: Aleyrodidae) Genet. Mol. Biol. Azevedo,Andréia Cristiane Souza; Sosa-Gómez,Daniel Ricardo; Faria,Marcos Rodrigues; Fungaro,Maria Helena Pelegrinelli. Bands of double-stranded RNA (dsRNA) were detected in three out of twelve isolates of Paecilomyces fumosoroseus. Identity of these bands was confirmed by RNAse, DNAse and S1 nuclease treatments. The cure of dsRNA for one isolate (P92) was successfully carried out for a single conidium subculture. Isogenic strains, with or without dsRNA, were submitted to virulence tests against the whitefly Bemisia tabaci strain B. In contrast to findings for some phytopathogenic fungi, these dsRNA fragments did not cause hypovirulence in P. fumosoroseus. Tipo: Info:eu-repo/semantics/other Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100010 Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates Scientia Agricola Azevedo,Andréia Cristiane Souza; Furlaneto,Márcia Cristina; Sosa-Gómez,Daniel Ricardo; Fungaro,Maria Helena Pelegrinelli. ITS and RAPD analyses were used to investigate molecular variations within samples of Paecilomyces isolates and to resolve five morphologically atypical isolates resembling P. fumosorosus, obtained from whitefly in Northern Paraná State. The ITS4-ITS5 amplicon was 700 base pairs (bp) long in all isolates. The five isolates of Paecilomyces not assigned to species produced restriction profiles identical to all the reference strains of P. fumosoroseus. The extent of fingerprint variability observed by RAPD was sufficient to discriminate all the isolates. The genetic similarity among unidentified isolates and strains of P. fumosoroseus was even higher than that observed among reference strains of this species, allowing us to conclude that isolates CNPso-P77,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Paecilomyces fumosoroseus; Bemisia; RAPD; Biological control; Entomopathogenic fungus. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400021 Registros recuperados: 2 Primeira ... 1 ... Última

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