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Validation of a customized subset of SNPs for sheep breed assignment in Brazil PAB
Paim,Tiago do Prado; McManus,Concepta; Vieira,Fábio Danilo; Oliveira,Stanley Robson de Medeiros; Facó,Olivardo; Azevedo,Hymerson Costa; Araújo,Adriana Mello de; Moraes,José Carlos Ferrugem; Yamagishi,Michel Eduardo Beleza; Carneiro,Paulo Luiz Souza; Caetano,Alexandre Rodrigues; Paiva,Samuel Rezende.
Abstract: The objective of this work was to evaluate the usefulness of a subset of 18 single nucleotide polymorphisms (SNPs) for breed identification of Brazilian Crioula, Morada Nova (MN), and Santa Inês (SI) sheep. Data of 588 animals were analyzed with the Structure software. Assignments higher than 90% confidence were observed in 82% of the studied samples. Most of the low-value assignments were observed in MN and SI breeds. Therefore, although there is a high reliability in this subset of 18 SNPs, it is not enough for an unequivocal assignment of the studied breeds, mainly of hair breeds. A more precise panel still needs to be developed for the widespread use in breed assignment.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovis aries; Animal genetic resources; Certification of origin; Genomics; Traceability.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000104302
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Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep PAB
Sousa Junior,Luis Paulo Batista; Meira,Ariana Nascimento; Azevedo,Hymerson Costa; Muniz,Evandro Nevez; Coutinho,Luiz Lehmann; Mourão,Gerson Barreto; Pedrosa,Victor Breno; Pinto,Luís Fernando Batista.
Abstract: The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovis aries; Candidate genes; Frequencies; Variants.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102810
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Variants in GH, IGF1, and LEP genes associated with body traits in Santa Inês sheep Scientia Agricola
Machado,Alessandro Lima; Meira,Ariana Nascimento; Jucá,Adriana de Farias; Azevedo,Hymerson Costa; Muniz,Evandro Neves; Coutinho,Luiz Lehmann; Mourão,Gerson Barreto; Pedrosa,Victor Breno; Pinto,Luís Fernando Batista.
ABSTRACT: Growth hormone (GH), insulin-like growth factor 1 (IGF1), and leptin (LEP) can be candidate genes for association studies because they play vital roles in the metabolism process. Thus, this study aimed to identify variants in these genes associated with body traits in Santa Inês sheep. The following were recorded: body weight at 100 (BW100) and 240 days (BW240), average daily gain (ADG), withers (WH) and croup (CH) heights, body length (BL), thoracic (TG) and leg (LG) girths, thoracic (TW) and croup (CW) widths, body depth (BD), rib eye area (REA), fat thickness (FT), and carcass finishing score (CFS). Single-locus association analysis was performed with 11 variants in IGF1, 18 in LEP, and 16 in GH. Moreover, two haplotypes in IGF1 and one...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hormone; Carcass; Growth; Haplotype; Ovine.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000300401
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Rapid serum agglutination and agar gel immunodiffusion tests associated to clinical signs in rams experimentally infected with Brucella ovis Ciência Rural
Nozaki,Cristiane Nakada; Lira,Nair Silva Cavalcanti de; Augusto Filho,Otávio; Azevedo,Hymerson Costa; Rodello,Leandro; Dasso,Maurício Gaudério; Bicudo,Sony Dimas; Antunes,João Marcelo Azevedo de Paula; Megid,Jane.
The purpose of this study was to evaluate the agar gel immunodiffusion and the rapid serum agglutination tests associated to clinical signs in rams experimentally infected with Brucella ovis. The serological profile during the 12 months of infection showed a large fluctuation of antibodies that favors the failure in the diagnostic. The evaluation of tests after the experimental infection allowed to suggest that none of the tests were able to detect the infection throughout the period of study. The study reinforces the importance of considering the clinical signs to support the diagnosis of Brucella ovis infection in rams.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovine infectious epididimitis; Sheep brucellosis; Diagnostic.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800023
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Estimação de parâmetros genéticos de ovinos da raça Santa Inês utilizando modelos uni e bicaracterística Ciência Rural
Carvalho,Geane Conceição; Barbosa,Leandro Teixeira; Oliveira,Tatiana Maia de; Fonseca,Fernanda Érica Pereira; Muniz,Evandro Neves; Azevedo,Hymerson Costa.
Objetivou-se nesse trabalho, estimar os parâmetros genéticos para características de crescimento em ovinos da raça Santa Inês através do Método da Máxima Verossimilhança Restrita (REML). Os componentes de (co)variâncias e os parâmetros genéticos foram estimados pelo Software MTDFREML (Multiple Trait Derivative-Free Restricted Maximum Likelihood). As características avaliadas em modelos uni e bicaracterística foram: peso ao nascimento (PN) e peso ao desmame (P90). Além dos efeitos fixos de sexo, grupo contemporâneo e tipo de parto, foram utilizados os seguintes efeitos aleatórios: efeito genético aditivo direto, efeito genético aditivo materno e efeito residual. As estimativas de herdabilidade aditiva direta para PN foram 0,20 e 0,21, para os modelos uni e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Correlação genética; Efeito materno; Herdabilidade; Modelo animal; Ovinos deslanados.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000100018
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