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Alternative complement pathway and factor B activities in rats with altered blood levels of thyroid hormone BJMBR
Bitencourt,C.S.; Duarte,C.G.; Azzolini,A.E.C.S.; Assis-Pandochi,A.I..
Evaluating the activity of the complement system under conditions of altered thyroid hormone levels might help elucidate the role of complement in triggering autoimmune processes. Here, we investigated alternative pathway (AP) activity in male Wistar rats (180 ± 10 g) after altering their thyroid hormone levels by treatment with triiodothyronine (T3), propylthiouracil (PTU) or thyroidectomy. T3 and thyroxine (T4) levels were determined by chemiluminescence assays. Hemolytic assays were performed to evaluate the lytic activity of the AP. Factor B activity was evaluated using factor B-deficient serum. An anti-human factor B antibody was used to measure factor B levels in serum by radial immunodiffusion. T3 measurements in thyroidectomized animals or animals...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thyroid hormone; Complement system; Alternative complement pathway; Factor B; T3; T4.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300006
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