|
|
|
|
|
| Baracat-Pereira,Maria Cristina; Barbosa,Meire de Oliveira; Magalhães Júnior,Marcos Jorge; Carrijo,Lanna Clicia; Games,Patrícia Dias; Almeida,Hebréia Oliveira; Sena Netto,José Fabiano; Pereira,Matheus Rodrigues; Barros,Everaldo Gonçalves de. |
| The enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general complex, and contaminants interfere with the identification of proteins involved in important physiological processes, such as plant defense against pathogens. This review discusses the challenges and strategies of separomics applied to the identification of low-abundance proteins and peptides in plants, especially in plants challenged by pathogens. Separomics is described as a group of methodological strategies for the separation of protein molecules for proteomics.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sample preparation; Complex protein extract; Subproteomes; Low-abundance proteins; Cell wall proteins. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200009 |
| |
|
|
| Almeida,Hebréia Oliveira; Barbosa,Meire de Oliveira; Marques,Ana Ermelinda; Pereira,Tânus Henrique Abdalla; Magalhães Júnior,Marcos Jorge; Tessarollo,Nayara Gusmão; Games,Patrícia Dias; Barros,Everaldo Gonçalves de; Stolf-Moreira,Renata; Marcelino-Guimarães,Francismar Corrêa; Abdelnoor,Ricardo Vilela; Pereira,Paulo Roberto Gomes; Baracat-Pereira,Maria Cristina. |
| O objetivo deste trabalho foi avaliar, por meio de enzimas marcadoras, a indução de resistência à ferrugem-asiática-da-soja em genótipos de soja contrastantes quanto à suscetibilidade a Phakopsora pachyrhizi. Aproteína total e as atividades de cinco enzimas marcadoras da indução de resistência (lipoxigenases, peroxidases, fenilalanina amônia-liase, quitinases e β-1, 3-glucanases) foram avaliadas em extratos de folhas de plantas de soja dos genótipos Embrapa 48 (suscetível) e PI 561356 (resistente), submetidas à inoculação ou não com o patógeno. Foram observadas respostas de defesa discrepantes entre os dois genótipos e entre os tempos de coleta (12, 72 e 168 horas após inoculação). A resposta de indução dessas enzimas assemelha-se à defesa bifásica, para... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Phakopsora pachyrhizi; Atividade enzimática; Mecanismos de defesa; Resistência sistêmica; Resposta em cascata. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000200003 |
| |
|
|
| Halfeld-Vieira,Bernardo de Almeida; Vieira Júnior,José Roberto; Romeiro,Reginaldo da Silva; Silva,Harllen Sandro Alves; Baracat-Pereira,Maria Cristina. |
| The objective of this work was to verify if the induced resistance mechanism is responsible for the capacity of a phylloplane resident bacteria (Bacillus cereus), isolated from healthy tomato plants, to control several diseases of this crop. A strain of Pseudomonas syringae pv. tomato was used as the challenging pathogen. The absence of direct antibiosis of the antagonist against the pathogen, the significant increase in peroxidases activity in tomato plants exposed to the antagonist and then inoculated with the challenging pathogen, as well as the character of the protection, are evidences wich suggest that biocontrol efficiency presented by the antagonist in previous works might be due to induced systemic resistance (ISR). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lycopersicon esculentum; Solanum esculentum; Biological control. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000800006 |
| |
|
|
|
