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Nitrogenase activity of Beijerinckia derxii is preserved under adverse conditions for its growth BJM
Barbosa,Heloiza R.; Moretti,Marcos A.; Thuler,Daniela S.; Augusto,Elisabeth F.P..
In order to evaluate the response of Beijerinckia derxii ICB-10 to different environmental factors, growth curves and specific nitrogenase activity were studied. Tested conditions were as follows: a) media with different pH values (2.5, 2.8, 4.2 and 5.7); b) medium supplemented with 230 µM aluminium sulphate; c) media with two different potassium phosphate concentrations (50 mM and 100 mM); d) shaken or still cultures; e) medium supplemented with 40 mM sodium thiosulphate. Growth curves and specific nitrogenase activity at pH 4.2 were closely similar to those for cultures at pH 5.7 (standard condition), whereas no growth occurred at pH 2.5. Changes in growth curves and/or specific nitrogenase activity were observed under the following conditions: I) pH 2.8...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beijerinckia; Nitrogenase; Nitrogen fixation; Growth; Environmental factors.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300007
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Beijerinckia derxii stimulates the viability of non-N2-fixing bacteria in nitrogen-free media BJM
Barbosa,Heloiza R.; Thuler,Daniela Strauss; Shirakawa,Márcia Aiko; Miyasaka,Natália R. S..
The interactions between the nitrogen-fixing microorganism Beijerinckia derxii with two non-diazotrophic bacteria, either Escherichia coli or a facultative sulphur-oxidizing chemolitotroph, were studied in mixed cultures. Direct and indirect contact between B. derxii and E. coli were tested. B. derxii increased CFU numbers and/or maintained the viability of the non-diazotrophic bacteria, but neither growth nor nitrogenase activity of the nitrogen-fixing bacterium were affected by either partner.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beijerinckia derxii; Coculture; Viability.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300004
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Molecular characterization of nitrogen-fixing bacteria isolated from brazilian agricultural plants at São Paulo state BJM
Reinhardt,Érica. L.; Ramos,Patrícia L.; Manfio,Gilson P.; Barbosa,Heloiza R.; Pavan,Crodowaldo; Moreira-Filho,Carlos A..
Fourteen strains of nitrogen-fixing bacteria were isolated from different agricultural plant species, including cassava, maize and sugarcane, using nitrogen-deprived selective isolation conditions. Ability to fix nitrogen was verified by the acetylene reduction assay. All potentially nitrogen-fixing strains tested showed positive hybridization signals with a nifH probe derived from Azospirillum brasilense. The strains were characterized by RAPD, ARDRA and 16S rDNA sequence analysis. RAPD analyses revealed 8 unique genotypes, the remaining 6 strains clustered into 3 RAPD groups, suggesting a clonal origin. ARDRA and 16S rDNA sequence analyses allowed the assignment of 13 strains to known groups of nitrogen-fixing bacteria, including organisms from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Diazotrophs; Selective isolation; Molecular systematics.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300002
Registros recuperados: 3
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