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Factors affecting the production and regeneration of protoplasts from Colletotrichum lindemuthianum Ciência e Agrotecnologia
Ishikawa,Francine Hiromi; Barcelos,Quélen de Lima; Souza,Elaine Aparecida de; Dias,Eustáquio Souza.
The present work reports factors affecting the production and regeneration of protoplasts from Colletotrichum lindemuthianum. The usefulness of protoplast isolation is relevant for many different applications and has been principally used in procedures involving genetic manipulation. Osmotic stabilizers, lytic enzymes, incubation time and mycelial age were evaluated in terms of their effects on protoplast yield. The optimal condition for protoplast production included the incubation of young mycelia (48 h) in 0.6 mol l-1 NaCl as the osmotic stabilizer, with 30 mg ml-1 Lysing Enzymes from Trichoderma harzianum for 3 h of incubation. In these conditions protoplasts production was higher than 10(6) protoplatos ml-1 in the digestion mixture, number suitable...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protoplast formation; Lytic enzyme; Anthracnose; Osmotic stabilizer; Filamentous fungi.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000100009
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