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Bosso,Patrícia Alves Ramos; Candeias,João Manuel Grisi; Paduan,Karina dos Santos; Ricchetti,Sandra Mara Queiroz; Miranda,Antero Frederico Macedo de; Rugolo,Lígia Maria Suppo de Souza; Durigon,Edison Luiz; Ventura,Armando Morais. |
Human Respiratory Syncytial Virus (HRSV) is recognized as the single most important pathogen responsible for acute viral infection of the lower respiratory tract in young children. In this study, 84 samples from children under two years of age presenting acute respiratory symptoms were obtained from September 2000 to November 2001. Analysis by immunofluorescence and reverse-transcription followed by PCR, revealed that 18% (15/84) of all the samples were positive for HRSV, in which 80% (12/15) of the cases was observed in children under six months of age and the subgroups A and B co-circulated in the studied period. These are the first data obtained for Botucatu city and seasonality is evident by the higher circulation of the virus between May and July. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human Respiratory Syncytial Virus (HRSV); RT-PCR; Immunofluorescence; Seasonality. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300015 |
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Campos,Angélica Cristine de Almeida; Durigon,Edison Luiz; Leal,Andréa Lima; Silva,Tereza Souza; Bosso,Patrícia Alves Ramos; Moraes,Claudia Trigo Pedroso; Oliveira,Danielle Bruna Leal; Lima,Hildener Nogueira; Vieira,Sandra Elisabete; Botosso,Viviane Fongaro; Zanotto,Paolo Marinho de Andrade; Stewien,Klaus Eberhard. |
The Human Respiratory Syncytial Virus (HRSV), isolated in 1955, is the main cause of hospitalization of babies and infants with respiratory illness. Several studies have been conducted worldwide aiming the development of a safe and effective vaccine against HRSV. The G2 region of glycoprotein G is used as genotyping default. In the present study, we performed a phylogenetic analysis of G protein and a comparative study between G2 region and ectodomain of attachment glycoprotein. Fifty-three nasal swab samples from children less than 5 years old and presenting symptoms of acute respiratory illness, assisted at the University Hospital (UH) of University of Sao Paulo (USP) in 2004, were submitted to sequencing by PCR and compared with GenBank sequences. We... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human Respiratory Syncytial Virus; G Glycoprotein; G2 Region; Phylogenetic Analysis. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300005 |
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