|
|
| Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R.. |
| The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and... |
| Tipo: Text |
Palavras-chave: Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00101/21195/19415.pdf |
| |
