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| Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Campos,Magnólia A.; Teixeira,Juliana E.C.; Targon,Maria Luísa P.N.; Carlos,Eduardo F.; Ravasi,Juliano F.; Fischer,Carlos N.; Machado,Marcos A.. |
| The Citrus ESTs Sequencing Project (CitEST) conducted at Centro APTA Citros Sylvio Moreira/IAC has identified and catalogued ESTs representing a set of citrus genes expressed under relevant stress responses, including diseases such as citrus variegated chlorosis (CVC), caused by Xylella fastidiosa. All sweet orange (Citrus sinensis L. Osb.) varieties are susceptible to X. fastidiosa. On the other hand, mandarins (C. reticulata Blanco) are considered tolerant or resistant to the disease, although the bacterium can be sporadically detected within the trees, but no disease symptoms or economic losses are observed. To study their genetic responses to the presence of X. fastidiosa, we have compared EST libraries of leaf tissue of sweet orange Pêra IAC (highly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ESTs; Citrus variegated chlorosis; Biotic stress; CitEST; Phytopathogen. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500024 |
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| Rosa,Daniel D.; Campos,Magnólia A.; Targon,Maria Luisa P.N.; Souza,Alessandra A.. |
| Due to the economic importance of gummosis disease for the citriculture, studies on P. parasitica-Citrus interaction comprise a significant part in the Brazilian Citrus genome data bank (CitEST). Among them, two cDNA libraries constructed from two different growth conditions of the P. parasitica pathogen are included which has generated the PP/CitEST database (CitEST - Center APTA Citros Sylvio Moreira/IAC- Millennium Institute). Through this genomic approach and clustering analyses the following has been observed: out of a total of 13,285 available in the Phytophthora parasitica database, a group of 4,567 clusters was formed, comprising 2,649 singlets and 1,918 contigs. Out of a total of 4,567 possible genes, only 2,651 clusters were categorized; among... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus disease; Elicitins; Plant pathogen; Gene expression profiles. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500028 |
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| Campos,Magnólia A.; Rosa,Daniel D.; Teixeira,Juliana Érika C.; Targon,Maria Luisa P.N.; Souza,Alessandra A.; Paiva,Luciano V.; Stach-Machado,Dagmar R.; Machado,Marcos A.. |
| In silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Functional genome; Ests; Gene expression profiles; Pathogenesis-related proteins; Defense genes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500020 |
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| Romano,Eduardo; Ferreira,Adriana T.; Dusi,André N.; Proite,Karina; Buso,Jose A.; Ávila,Antonio C.; Nishijima,Marta L.; Nascimento,Adriana S.; Bravo-Almonacid,Fernando; Mentaberry,Alejandro; Monte,Damares; Campos,Magnólia A.; Melo,Paulo Eduardo; Cattony,Monica K.; Torres,Antonio C.. |
| The coat protein (CP) gene of the potato virus Y strain "o" (PVY O) was introduced into potato, cultivar Achat, via Agrobacterium tumefaciens-mediated transformation. Sixty three putative transgenic lines were challenged against the Brazilian strains PVY-OBR and PVY-NBR. An extremely resistant phenotype, against the two strains, was observed in one line, denominated 1P. No symptoms or positive ELISA results were observed in 16 challenged plants from this line. Another clone, named as 63P, showed a lower level of resistance. Southern blot analysis showed five copies of the CP gene in the extremely resistant line and at least three copies in the other resistant line. The stability of the integrated transgenes in the extreme resistant line was examined during... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; GMO. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362001000200004 |
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