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	Provedor de dados Agronomy (2) Autor Ceccon, Cassia Canzi (2) Didoné, Dielli Aparecida (2) Grando, Magali Ferrari (2) Silva, Marília Rodrigues de (2) Suzin, Marilei (2) Teixeira, Tiago (2) Mais... Palavra-chave GUS histochemical assay (2) Genetic engineering (2) 5.01.03.00-8 Fitotecnia agrobacteria (1) Agrobacteria (1) Biotecnologia Vegetal (1) Zea mays. (1) Zea mays. Biotecnologia Vegetal (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Increased transient genetic transformation in immature embryos of Brazilian BR 451 maize co-cultivated with Agrobacterium tumefaciens Agronomy Didoné, Dielli Aparecida; Silva, Marília Rodrigues de; Ceccon, Cassia Canzi; Teixeira, Tiago; Suzin, Marilei; Grando, Magali Ferrari.  Genetic engineering has amplified the possibilities of crop breeding and supported sustainability ideals. Agrobacterium tumefaciens is the preferred transformation system, since it produces transgenic plants with more stable transgene expression and inheritance. The agrobacteria and plant tissue must be co-cultivated in conditions that allow gene transfer. This study aimed to evaluate how co-cultivation time and temperature affect the transformation of immature maize embryos of the Hi-II hybrid (model genotype) and the Brazilian BR 451 variety with A. tumefaciens EHA101:pTF102. The pTF102 plasmid carries a uidA reporter gene that enables transient transformation to be quickly verified by GUS histochemical assays. Increasing the co-cultivation period from... Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.00-8 Fitotecnia agrobacteria; GUS histochemical assay; Genetic engineering; Zea mays. Biotecnologia Vegetal. Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/36475 Increased transient genetic transformation in immature embryos of Brazilian BR 451 maize co-cultivated with Agrobacterium tumefaciens Agronomy Didoné, Dielli Aparecida; Silva, Marília Rodrigues de; Ceccon, Cassia Canzi; Teixeira, Tiago; Suzin, Marilei; Grando, Magali Ferrari.  Genetic engineering has amplified the possibilities of crop breeding and supported sustainability ideals. Agrobacterium tumefaciens is the preferred transformation system, since it produces transgenic plants with more stable transgene expression and inheritance. The agrobacteria and plant tissue must be co-cultivated in conditions that allow gene transfer. This study aimed to evaluate how co-cultivation time and temperature affect the transformation of immature maize embryos of the Hi-II hybrid (model genotype) and the Brazilian BR 451 variety with A. tumefaciens EHA101:pTF102. The pTF102 plasmid carries a uidA reporter gene that enables transient transformation to be quickly verified by GUS histochemical assays. Increasing the co-cultivation period from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacteria; GUS histochemical assay; Genetic engineering; Zea mays.; Biotecnologia Vegetal. Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/36475 Registros recuperados: 2 Primeira ... 1 ... Última

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