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| Wang,Jianmin; Song,Ruihua; Chen,Lei; Yin,Liangjun; Chen,Bo; Sun,Jing; Gou,Yuanbing; Zhao,Ling; Chen,Lin. |
| Objective: To investigate the functions of Fibroblast Growth Factor Receptor-2 (FGFR2) at different stages of cell differentiation. The engineered murine embryonic stem (ES) cells with conditional knockout of FGFR2 were developed depending on Cre-loxP. Methods: Cre-loxP system was used in a conditional targeting vector. The competent AM-1 bacteria, which expressed Cre-recombinase, was used to confirm the Cre-mediated deletion of the floxed exons 7 and 8 of FGFR2. The targeting vector was electroporated into the ES cells, and the transfected ES cells were screened with G418 and Ganciclovir. Finally, the ES clones with correct targeting events were identified by Southern Blot and PCR. Results: The targeting vector with conditional knockout of murine FGFR2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibroblast growth factor receptor-2 (FGFR2); Conditional knockout; Homologous recombination; Cre; ES cells. |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200002 |
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| Yu,Guangchao; Chen,Lei; Lin,Chii-wann; Li,Bing; Cui,Hemiao; Chen,Siyi; Miao,Jian; Bian,Huawei; Chen,Dingqiang; Deng,Yang. |
| BACKGROUND: The occurrence and prevalence of integrons in clinical microorganisms and their role played in antimicrobial resistance have been well studied recently. As screening and detection of integrons are concerned, current diagnostic methodologies are restricted by significant drawbacks and novel methods are required for integrons detection. RESULTS: In this study, three loop-mediated isothermal amplification (LAMP) assays targeting on class 1, 2 and 3 integrons were implemented and evaluated. Optimization of these detection assays were performed, including studing on the reaction temperature, volume, time, sensitivity and specificity (both primers and targets). Application of the established LAMP assays were further verified on a total of 1082... |
| Tipo: Journal article |
Palavras-chave: Loop-mediated isothermal amplification (LAMP); Integron screening; Bacterial integrons; Class 1 integron; Class 2 integron; Class 3 integron. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100076 |
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| Dong,Zhihao; Li,Junfeng; Chen,Lei; Wang,Siran; Shao,Tao. |
| ABSTRACT: This study was conducted to evaluate the effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (TBL). The TBL leaves silage was either untreated (control) or treated with 1 × 106 cfu/g FM Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), or a combination of 3% molasses and Lactobacillus plantarum (L+M). Fermentation quality, chemical composition and nutrient digestibility were then analyzed. Ethanol and acetic acid concentrations were the dominant fermentation products in all silages except L+M silage. The L, G and L+G treatments failed to influence the fermentation. The M treatment increased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Additives; Fermentation quality; In vitro digestibility; Chemical composition; Tetraploid black locust.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700201 |
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