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DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant? ArchiMer
Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R..
The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and...
Tipo: Text Palavras-chave: Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00101/21195/19415.pdf
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A biofilm-forming marine bacterium producing proteins as extracellular polymeric substances ArchiMer
Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, François; Compere, Chantal; Combes, Didier.
EPS biochemical characterization and observation by epifluorescencemicroscopy. Development of a marine biofilmformation model onto a polystyrene microtiterplate using the “O’Tooleand Koltermethod”[1] adapted with the well known fluorescent DNA staining DAPI
Tipo: Text
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00089/19998/17661.pdf
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Biosensors for the detection and monitoring of Alexandrium minutum along the French coasts ArchiMer
Antoine, Elisabeth; Compere, Chantal; Cosnier, Serge; Crassous, Marie-pierre; Derbal, Hassina; Erard-le Denn, Evelyne; Gondran, Chantal; Guilminot, Elodie; Lehaitre, Michel; Mazeas, Florence; Moreau, Hervé; Perrot, Hubert; Pradier, Claire-marie; Salmain, Michèle.
The microalgae Alexandrium minutum produces neurotoxins responsible for paralytic shellfish poisoning, which upon accumulation in shellfish represent a human health risk. This species recurrently forms toxic blooms in the Northern part of the French Brittany coast. At present, algae identification relies on tedious microscope observations mostly performed by skilled taxonomists in laboratories. Newly emerging fields of molecular taxonomy, nanoscale technology and biotechnology offer opportunities for the development of in-situ biosensors for the detection and the monitoring of toxic algae.
Tipo: Text
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00089/19986/17650.pdf
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Monoclonal antibody against the surface of Alexandrium minutum used in a whole-cell ELISA ArchiMer
Gas, F; Pinto, L; Baus, B; Gaufres, L; Crassous, Marie-pierre; Compere, Chantal; Quemeneur, E.
Harmful algal blooms represent a major threat to marine production, and particularly to shellfish farming. Alexandrium minutum, which causes paralytic shellfish poisoning, is occurring with increasing frequency along European coasts. Current regulatory methods to analyze environmental samples are tedious and time consuming because they require taxonomists and involve animal experiments. New rapid detection methods, such as immunoassays, are needed to ensure a fast alert system and for field studies of algal ecodynamics. Rat monoclonal antibodies were raised and selected for their ability to specifically recognize a surface antigen for the A. minutum strain AM89BM from the Bay of Morlaix, France. A whole-cell ELISA was designed, leading to the selection of...
Tipo: Text Palavras-chave: Monoclonal antibody; Harmful algal bloom; Enzyme linked immunosorbent assay; Dinoflagellate.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2008/publication-4647.pdf
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Building of an immunosensor: How can the composition and structure of the thiol attachment layer affect the immunosensor efficiency? ArchiMer
Briand, Elisabeth; Salmain, Michèle; Henry, Jean-marie; Perrot, Hubert; Compere, Chantal; Pradier, Claire-marie.
Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved.
Tipo: Text Palavras-chave: QCM; IRRAS; Thiol attachment; Immunosensor; Mixed SAM' s.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1988.pdf
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Direct detection of toxic micro-algae Alexandrium minutum through high sensitive gravimetric sensors ArchiMer
Bucur, Bogdan; Lazerges, Mathieu; Perrot, Hubert; Antoine, Elisabeth; Hamelin, Muriel; Compere, Chantal.
In this work QCM technologies were used to set up a DNA based biosensor dedicated to the detection of toxic micro-algae in the environment. Up to now, appropriate detection were not achieved for different reasons as the possibility of regeneration, the capability of multidetection or the detection of very low DNA target concentration. For these reasons different configurations were examined based on in-situ and fast regeneration, supramolecular chemistry of DNA and high sensitive gravimetric devices. In a first step an OligoDesoxyRibonucleotide 5’ tailed with a disulfide group (SH-ODN) designed to be a specific molecular probe for the toxic micro-algae Alexandrium minutum was grafted on the quartz surface resonators. Kinetics of probe immobilization and of...
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Ano: 2008 URL: http://archimer.ifremer.fr/doc/00089/19996/17659.pdf
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Biofouling protection for marine environmental sensors ArchiMer
Delauney, Laurent; Compere, Chantal; Lehaitre, Michel.
These days, many marine autonomous environment monitoring networks are set up in the world. These systems take advantage of existing superstructures such as offshore platforms, lightships, piers, breakwaters or are placed on specially designed buoys or underwater oceanographic structures. These systems commonly use various sensors to measure parameters such as dissolved oxygen, turbidity, conductivity, pH or fluorescence. Emphasis has to be put on the long term quality of measurements, yet sensors may face very short-term biofouling effects. Biofouling can disrupt the quality of the measurements, sometimes in less than a week. Many techniques to prevent biofouling on instrumentation are listed and studied by researchers and manufacturers. Very few of them...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00008/11920/8626.pdf
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Biofouling protection for marine underwater observatories sensors ArchiMer
Delauney, Laurent; Compere, Chantal; Lehaitre, Michel.
These days, many marine autonomous environment monitoring networks are set up in the world. Such systems take advantage of existing superstructures such as offshore platforms, lightships, piers, breakwaters or are placed on specially designed buoys or deep sea fix stations. The major goal for these equipments is to provide in real time reliable measurements without costly frequent maintenance. These autonomous monitoring systems are affected by a well known phenomena in seawater condition which is biofouling. Consequently, such system without efficient biofouling protection is hopeless. This protection must be applied to the sensors and to the underwater acoustic communication equipments.
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00107/21801/20176.pdf
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Surface enhanced infrared absorption (SEIRA) spectroscopy using gold nanoparticles on As2S3 glass ArchiMer
Verger, F.; Pain, T.; Nazabal, V.; Boussard-pledel, C.; Bureau, Bruno; Colas, Florent; Rinnert, Emmanuel; Boukerma, Kada; Compere, Chantal; Guilloux-viry, M.; Deputier, S.; Perrin, A.; Guin, J. P..
The development of infrared sensing platform requires adapted materials. In this work, we used sputtered gold nanoparticles on a chalcogenide glass to demonstrate that these infrared materials can be used as substrate for surface enhanced infrared absorption (SEIRA) spectroscopy. The sputtering parameters were optimized to get the highest possible enhancement. To assess it, a self-assembled monolayer of 4-nitrothiophenol was used. These preliminary results are promising and pave the way for various configurations of waveguide for integrated optical components. (C) 2012 Elsevier B.V. All rights reserved.
Tipo: Text Palavras-chave: Chalcogenide glass; Sputtering; Gold nanoparticles; 4-Nitrothiophenol; SEIRA; Surface enhanced infrared absorption.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00116/22748/20613.pdf
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Direct and fast detection of Alexandrium minutum algae by using high frequency microbalance ArchiMer
Sousa, Celia; Compere, Chantal; Dreanno, Catherine; Crassous, Marie-pierre; Gas, Fabienne; Baus, Beatrice; Perrot, Hubert.
In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes.
Tipo: Text Palavras-chave: Alexandrium minutum; Quartz microbalance; Microalgae; Immunosensor.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00194/30492/28897.pdf
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Immobilization of Protein A on SAMS for the elaboration of immunosensors ArchiMer
Briand, Elisabeth; Salmain, Michèle; Compere, Chantal; Pradier, Claire-marie.
Binary mixtures of 11-mercaptoundecanoic acid (MUA) and other thiols of various lengths and terminal functions were chemisorbed on gold-coated surfaces via S-Au bonds to form mixed self-assembled monolayers (SAMs). Several values of the inole fraction of MUA in the thiol mixtures were tested and the structure and composition of the resulted thin films were characterized by X-ray photoelectron spectroscopy (XPS) and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). The results made it clear that co-adsorption of MUA with thiols of similar chain length led to well-ordered monolayers whereas the co-adsorption of MUA with shorter thiols yielded less crystalline-like thin films, but with more reactive carboxylic acid terminal...
Tipo: Text Palavras-chave: PM IRRAS; Antibody; Protein A; Gold; Self assembled monolayers.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2222.pdf
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In situ QCM DNA-biosensor probe modification ArchiMer
Lazerges M, M; Perrot, H; Zeghib N, N; Antoine, Elisabeth; Compere, Chantal.
We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA...
Tipo: Text Palavras-chave: QCM; DNA; Biosensor.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1985.pdf
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NOUBA Project Novel Natural Molecules Inhibiting the Development of Marine Bacteria in Biofilms ArchiMer
Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain.
Marine organisms represent a rather unexplored source of new activity and biological functions of molecules for biotechnologies. The research in chemical ecology of the marine environment leads to looking at metabolites of recognition or defence produced by these models. Marine bacteria belonging to the Pseudoalteromonas genus of the Gammaproteobacteria class are often found in association with marine eukaryotes, and their ability to produce a variety of biological activities attracted a particular attention. The marine Pseudoalteromonas sp. 3J6 and D41 were selected for their capacity to inhibit the biofilm formation of other bacteria. The study of antibiofilm metabolites synthesised by marine bacteria 3J6 and D41 biofilms can lead to the development of...
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00089/19990/17666.pdf
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Behaviour of stainless steel in natural seawater ArchiMer
Compere, Chantal; Le Bozec, Nathalie.
In this paper, investigations performed in natural and artificial seawater on stainless steels will be presented. They concerned studies on: biofilm formation, passive layers composition, electrochemical behaviour, localised corrosion and the evolution of these different parameters as a function of ageing time. According to literature surveys, the different aspects will be discussed. Some conclusions will be drawn concerning the actual knowledge on the behaviour of stainless steels in seawater.
Tipo: Text
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00089/19989/17653.pdf
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45-and 70-base DNA supramolecular polymerizations on quartz crystal microbalance biosensor ArchiMer
Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Antoine, Elisabeth; Compere, Chantal.
Supramolecular polymerizations of 45- and 70- base DNAs on the surface of an in-situ time-resolved 27 MHz quartz crystal microbalance biosensor.
Tipo: Text Palavras-chave: Sensor; Design; Kinetics; Immobilization; Hybridization.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1710.pdf
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A marine bacterial adhesion microplate test using the DAPI fluorescent dye: a new method to screen antifouling agents ArchiMer
Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Rochet, Marie-joelle; Compere, Chantal; Combes, D.
Aims: To develop a method to screen antifouling agents against marine bacterial adhesion as a sensitive, rapid, quantitative microplate fluorescent test. Methods and Results: Our experimental method is based on a natural biofilm formed by mono-incubation of the marine bacterium Pseudoalteromonas sp. D41 in sterile natural sea water in a 96 wells polystyrene microplate. The 4'6-diamidino-2-phenylindole (DAPI) dye was used to quantify adhered bacteria in each well. Total measured fluorescence in wells was correlated with the amount of bacteria showing a detection limit of one bacterium per 5 µm2 and quantifying 2 x 107 to 2 x 108 bacteria adhered per cm2. The antifouling properties of three commercial surface active agents and chlorine were tested by this...
Tipo: Text Palavras-chave: Antifouling; Pseudoalteromonas; Screening; Microtiter plate; Adhesion; DAPI; Marine biofilm.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2006/publication-2017.pdf
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Ficopomatus enigmaticus, Ecologie, répartition en Bretagne et en France, nuisances et moyens de lutte sur le site atelier du port de Vannes ArchiMer
Camus, Patrick; Compere, Chantal; Blanchet, Aline; Dimeet, Joel; Hamon, Dominique; Lacotte, Nicolas; Peleau, Michel; Lassalle, Elisabeth.
This study [...] was carried out from June 1998 to January 2000 and enabled the assessment of the scientific knowledge on the biology, ecology and distribution of the Annelida Polychaeta sedentaria: Ficopomatus enigmaticus. [...] In order to better determine the characteristics of this species' environment and development cycle, a study was conducted in the worksite of the marina of Vannes. This study, mostly biological, was completed by a technological study on the fighting techniques (antifouling paints) used against the damage made by this worm on the boat hulls. In addition, this document presents some recommendations to follow in order to limit, or stop, the development of this "pollution".
Tipo: Text Palavras-chave: Marina harbours; Disturbance; Colonization; Ficopomatus enigmaticus; Port de plaisance; Pertubation; Colonisation; Ficopomatus enigmaticus.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/rapport-6131.pdf
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Detection of polycyclic aromatic hydrocarbon (PAH) compounds in artificial sea-water using surface-enhanced Raman scattering (SERS) ArchiMer
Peron, Olivier; Rinnert, Emmanuel; Lehaitre, Michel; Crassous, Philippe; Compere, Chantal.
This paper reports an accurate synthesis of surface-enhanced Raman scattering (SERS) active substrates, based on gold colloidal monolayer, suitable for in situ environmental analysis. Quartz substrates were functionalized by silanization with (3-mercaptopropyl)trimethoxysilane (MPMS) or (3-aminopropyl)trimethoxysilane (APTMS) and they subsequently reacted with colloidal suspension of gold metal nanoparticles: respectively, the functional groups SH and NH2 bound gold nanoparticles. Gold nanoparticles were prepared by the chemical reduction of HAuCl4 using sodium tricitrate and immobilized onto silanized quartz substrates. Active substrate surface morphology was characterized with scanning electron microscopy (SEM) measurements and gold nanoparticles...
Tipo: Text Palavras-chave: Sensor; Sea water; PAH detection; SERS; Silanization; Gold nanoparticles.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6616.pdf
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Toward Detection of Harmful Algae Blooms by in situ Surface Plasmon Resonance Spectroscopy ArchiMer
Laurent, Sebastien; Colas, Florent; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Lehaitre, Michel; Compere, Chantal.
Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.
Tipo: Text Palavras-chave: PCR; Algal detection; In situ; Spectroscopy; Surface plasmon resonance.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6830.pdf
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Surface plasmon resonance in chalcogenide glass-based optical system ArchiMer
Le Person, Jenny; Colas, Florent; Compere, Chantal; Lehaitre, Michel; Anne, M; Boussard Pledel, C; Bureau, B; Adam, J; Deputier, S; Guilloux Viry, M.
The surface plasmon resonance phenomenon has been studied in a chalcogenide glass-based optical system. IR transmission properties of these materials combined to their high refractive indices lead to advantageous properties for sensing. In this study, numerical simulations have been carried out to investigate the potentialities of sulfide glass from the GeGaSbS system as a coupling prism material. Then, an angular modulation SPR biosensor has been set up in the Kretschmann-Raether arrangement. Experimental data are consistent with numerical calculation and the detection limit of the sensor is 3 x 10(-5) RIU. These preliminary results are promising. Further investigations have to be carried out to confirm the great potentialities of those materials for...
Tipo: Text Palavras-chave: Infrared; Biosensor; Surface plasmon resonance; Chalcogenide glass.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3955.pdf
Registros recuperados: 63
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