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Dynamics of proteins, carbohydrates and global DNA methylation patterns during induction of nodular cluster cultures from seeds of Vriesea reitzii Agronomy
Corredor, Jenny; De Conti, Daniela; Guerra, Miguel Pedro; Dal Vesco, Lirio Luiz; Pescador, Rosete.
Tissue culture techniques have been employed for bromeliad mass propagation by means of the morphogenetic route of nodular cluster cultures (NCs). This study aimed to assess protein, carbohydrate and global DNA methylation (GDM) level dynamics during NCs induction from Vriesea reitzii seeds. Seeds were inoculated into Murashige and Skoog (MS) liquid medium supplemented with 4 µM α-naphthaleneacetic acid (NAA) to induce NCs and in culture medium without plant growth regulators to form normal seedlings. Samples collected at 0, 3, 7, 10, 14, and 21 days of culture were analyzed. All parameters assessed showed the same variation pattern. However, seeds inducing NCs showed significantly lower starch (6.0 mg g-1 FM), carbohydrate (10.7 mg g-1 FM) and GDM (11.0%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromeliaceae; Bromeliad; Germination; Micropropagation; Seedling. Bromeliaceae bromeliad germination micropropagation seedling..
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42448
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