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In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE Ciência e Agrotecnologia
Couto,Evellyn Giselly de Oliveira; Pinho,Édila Vilela de Resende Von; Pinho,Renzo Garcia Von; Veiga,Adriano Delly; Bustamante,Fernanda de Oliveira; Dias,Kaio Olimpio das Graças.
ABSTRACTArtificial chromosome duplication is one of the most important process in the attainment of doubled haploids in maize. This study aimed to evaluate the induction ability of the inducer line KEMS in a tropical climate and test the efficiency of the R1-Navajo marker by flow cytometry to evaluate two chromosome duplication protocols and analyze the development of the doubled haploids in the field. To accomplish this goal, four genotypes (F1 and F2 generations) were crossed with the haploid inducer line KEMS. The seeds obtained were selected using the R1-Navajo marker and subject to two chromosome duplication protocols. Duplication was confirmed using flow cytometry. The percentages of self-fertilized plants after duplication as well as the quantities...
Tipo: Info:eu-repo/semantics/article Palavras-chave: R-Navajo; Colchicine; Induction of maternal haploids; Zea mays.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500435
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Identification of haploid maize by flow cytometry, morphological and molecular markers Ciência e Agrotecnologia
Couto,Evellyn Giselly de Oliveira; Davide,Livia Maria Chamma; Bustamante,Fernanda de Oliveira; Pinho,Renzo Garcia Von; Silva,Tallyta Nayara.
The development of homozygous breeding lines in maize may be accelerated through the use of haploids. Thus, the obtaining and prior identification of haploids generated by the haploid inducer lines is an important factor. The purpose of this study was to identify haploids by flow cytometry and to correlate the nuclear DNA content to the morphological and morphometric traits of the seeds that gave rise to them. In addition, molecular markers were used to confirm the androgenetic nature of the haploid. The seeds obtained were derived from the cross between the inbred line W23 and the commercial hybrid P30F90. Among these seeds, a group was selected, putative haploids, whose embryo was white and the pericarp purplish. This group, consisting of 330 seeds, was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Nuclear DNA content; Androgenetic inheritance; R-navajo; Microsatellites.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000100003
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