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Registros recuperados: 6
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Investigation of bacterial communities within the digestive organs of the hydrothermal vent shrimp Rimicaris exoculata provide insights into holobiont geographic clustering ArchiMer
Cowart, Dominique; Durand, Lucile; Cambon-bonavita, Marie-anne; Arnaud-haond, Sophie.
Prokaryotic communities forming symbiotic relationships with the vent shrimp, Rimicaris exoculata, are well studied components of hydrothermal ecosystems at the Mid-Atlantic Ridge (MAR). Despite the tight link between host and symbiont, the observed lack of spatial genetic structure seen in R. exoculata contrasts with the geographic differentiation detected in specific bacterial ectosymbionts. The geographic clustering of bacterial lineages within a seemingly panmictic host suggests either the presence of finer scale restriction to gene flow not yet detected in the host, horizontal transmission (environmental selection) of its endosymbionts as a consequence of unique vent geochemistry, or vertically transmitted endosymbionts that exhibit genetic...
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Ano: 2017 URL: https://archimer.ifremer.fr/doc/00376/48705/49058.pdf
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Metagenomic sequencing of environmental DNA reveals marine faunal assemblages from the West Antarctic Peninsula ArchiMer
Cowart, Dominique; Murphy, Katherine R.; Cheng, C. -h. Christina.
The West Antarctic Peninsula (WAP) is the fastest warming region in Antarctica where climate impact on the cold-adapted marine ecosystem is already visible. To monitor faunal changes in remote vast bodies of Antarctic waters, efficient and informative tools are essential. High-throughput sequencing of environmental DNA (eDNA) has emerged as one such tool for monitoring biodiversity and ecosystems, as it increases detection sensitivity of taxa, and sampling is often simpler and less costly than traditional collection methods. We collected water samples from four WAP shallow (≤ 300 m) shelf regions, recovered the eDNA therein, and performed metagenomic shotgun sequencing and analyses to determine the effectiveness of this method to assess marine benthic...
Tipo: Text Palavras-chave: EDNA; Antarctica; Metagenomics; King crabs; Benthic invertebrates.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00413/52490/53278.pdf
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Environmental DNA (eDNA) applications for the conservation of imperiled crayfish (Decapoda: Astacidea) through monitoring of invasive species barriers and relocated populations ArchiMer
Cowart, Dominique; Breedveld, Koert G. H.; Ellis, Maria J.; Hall, Joshua M.; Larson, Eric R..
Native crayfish species often face competition and displacement by non-indigenous invasive crayfishes. Management responses implemented to preserve imperiled crayfishes may include the construction of physical barriers to prevent the spread of invasive crayfishes, and movement of native populations to "ark" sites that have not yet been invaded. These strategies require ongoing monitoring to determine their effectiveness. We propose that environmental DNA (eDNA), genetic material identified from environmental samples, can be useful for assessing advancing invasions and imperiled freshwater species associated with management interventions. We monitored a series of management interventions intending to isolate the endangered Shasta crayfish Pacifastacus...
Tipo: Text Palavras-chave: Ark site; California; Endangered crayfishes; Pacifastacus fortis; Pacifastacus leniusculus; Translocation.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00443/55453/60475.pdf
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Exploring Environmental DNA (eDNA) to Assess Biodiversity of Hard Substratum Faunal Communities on the Lucky Strike Vent Field (Mid-Atlantic Ridge) and Investigate Recolonization Dynamics After an Induced Disturbance ArchiMer
Cowart, Dominique; Matabos, Marjolaine; Brandt, Miriam; Marticorena, Julien; Sarrazin, Jozee.
Deep ocean hydrothermal vent ecosystems face physical disturbances from naturally occurring volcanic and tectonic activities and are at increasing risk of mineral resource exploitation, raising concerns about the resilience of endemic biological communities. Following destructive events, efficient and rapidly applicable surveys of organisms are required to monitor the state, evolution and a possible return of these ecosystems to their original baseline status. In this study, we explored the environmental DNA (eDNA) approach as a tool (1) to assess biodiversity of benthic communities associated with deep-sea hard substrata and (2) tracked the recolonization dynamics of benthic invertebrate communities living on the Montségur edifice within the Lucky Strike...
Tipo: Text Palavras-chave: Natural regeneration; Clearance; Bathymodiolus azoricus; Monitoring; Hard substratum; Active and inactive vent sites.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00600/71176/69515.pdf
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Depth-dependent gene flow in Gulf of Mexico cold seep Lamellibrachia tubeworms (Annelida, Siboglinidae) ArchiMer
Cowart, Dominique; Halanych, Kenneth M.; Schaeffer, Stephen W.; Fisher, Charles R..
Lamellibrachia vestimentiferan tubeworms form aggregations at hydrocarbon cold seeps in the deep Gulf of Mexico (GoM), creating structures that provide living space for other fauna. In the GoM, three Lamellibrachia taxa vary in morphology and depth ranges: Lamellibrachia luymesi (300–950 m), Lamellibrachia sp. 1 (950–2,604 m), and Lamellibrachia sp. 2 (1,175–3,304 m). While Lamellibrachia sp. 2 is consistently identified as a separate species, L. luymesi and sp. 1 cannot be discriminated using barcoding markers cytochrome oxidase subunit 1 (COI) and large ribosomal subunit rDNA (16S). To determine if limited gene flow was a factor in the formation of these taxa, we employed more quickly evolving markers, including mitochondrial cytochrome B (CYTB),...
Tipo: Text Palavras-chave: Deep sea; Hydrocarbon seep; Microsatellite; Population structure; Siboglinid; Vestimentiferan tubeworm.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00194/30494/29935.pdf
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Restriction to large-scale gene flow vs. regional panmixia among cold seep Escarpia spp. (Polychaeta, Siboglinidae) ArchiMer
Cowart, Dominique; Huang, Chunya; Arnaud-haond, Sophie; Carney, Susan L.; Fisher, Charles R.; Schaeffer, Stephen W..
The history of colonization and dispersal in fauna distributed among deep-sea chemosynthetic ecosystems remains enigmatic and poorly understood because of an inability to mark and track individuals. A combination of molecular, morphological and environmental data improves understanding of spatial and temporal scales at which panmixia, disruption of gene flow or even speciation may occur. Vestimentiferan tubeworms of the genus Escarpia are important components of deep -sea cold seep ecosystems, as they provide long-term habitat for many other taxa. Three species of Escarpia, Escarpia spicata [Gulf of California (GoC)], Escarpia laminata [Gulf of Mexico (GoM)] and Escarpia southwardae (West African Cold Seeps), have been described based on morphology, but...
Tipo: Text Palavras-chave: Deep sea; Hydrocarbon seep; Microsatellite; Population structure; Siboglinid; Vestimentiferan tubeworm.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00152/26354/24515.pdf
Registros recuperados: 6
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