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| Quecini,Vera; Torres,Gisele A.M.; Rosa Jr,Vicente E. de; Gimenes,Marcos A.; Machado,Jeanne B. de M.; Figueira,Antonio V. de O.; Benedito,Vagner; Targon,Maria Luisa P.N.; Cristofani-Yaly,Mariângela. |
| Plant hormones play a crucial role in integrating endogenous and exogenous signals and in determining developmental responses to form the plant body throughout its life cycle. In citrus species, several economically important processes are controlled by phytohormones, including seed germination, secondary growth, fruit abscission and ripening. Integrative genomics is a powerful tool for linking newly researched organisms, such as tropical woody species, to functional studies already carried out on established model organisms. Based on gene orthology analyses and expression patterns, we searched the Citrus Genome Sequencing Consortium (CitEST) database for Expressed Sequence Tags (EST) consensus sequences sharing similarity to known components of hormone... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Defense responses; Development; Plant growth regulators. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500002 |
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| Palmieri,Darío Abel; Novelli,Valdenice Moreira; Bastianel,Marinês; Cristofani-Yaly,Mariângela; Astúa-Monge,Gustavo; Carlos,Eduardo Fermino; Oliveira,Antonio Carlos de; Machado,Marcos Antonio. |
| Nearly 65,000 citrus EST (Expressed Sequence Tags) have been investigated using the CitEST project database. Microsatellites were investigated in the unigene sequences from Citrus spp. and Poncirus trifoliata. From these sequences, approximately 35% of the non-redundant ESTs contained SSRs. The frequencies of different SSR motifs were similar between Citrus spp and trifoliate orange. In general, mononucleotide repeats appeared to be the most abundant SSRs in the CitEST database, but we also identify di-, tri-, tetra-, penta- and hexanucleotide repeats. The AG/CT and AAG/CTT were the most common dinucleotide and trinucleotide motifs, with frequencies of 54.4% and 25.2%, respectively. Primer sequences flanking SSR motifs were successfully designed and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus spp.; Poncirus trifoliata; ESTs; Microsatellites; In silico polymorphism. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500029 |
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| Cristofani-Yaly,Mariângela; Berger,Irving J.; Targon,Maria Luisa P.N.; Takita,Marco A.; Dorta,Sílvia de O.; Freitas-Astúa,Juliana; Souza,Alessandra A. de; Boscariol-Camargo,Raquel L.; Reis,Marcelo S.; Machado,Marcos A.. |
| Citrus is the most important fruit crop in Brazil and Citrus tristeza virus (CTV) is considered one of the most important pathogens of citrus. Most citrus species and varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative of citrus, is resistant to the virus. In order to better understand the responses of citrus plants to the infection of CTV, we constructed expressed sequence tag (EST) libraries with tissues collected from Poncirus trifoliata plants, inoculated or not with Citrus tristeza virus at 90 days after inoculation, grafted on Rangpur lime rootstocks. We generated 17,867 sequence tags from Poncirus trifoliata inoculated (8,926 reads) and not (8,941 reads) with a severe CTV isolate. A total of 2,782 TCs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Disease resistance; Citrus tristeza virus; Biotic stress. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500025 |
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| Latado,Rodrigo Rocha; Cristofani-Yaly,Mariângela; Carvalho,Carlos Roberto de; Machado,Marcos Antônio. |
| O objetivo deste trabalho foi obter plantas autotetraplóides de tangerina 'Ponkan', laranja 'Pêra-de-abril' e tangor 'Murcott', que serão usadas em cruzamentos com cultivares diplóides, visando à obtenção de indivíduos triplóides sem sementes. Utilizou-se o método de cultivo in vitro de segmentos de epicótilo em meio com colchicina (0,025, 0,05 e 0,1%), por diversos períodos (1, 2, 3, 7 e 14 dias), com subseqüente regeneração de brotações em meio sem a presença do alcalóide. As brotações foram microenxertadas in vitro e aclimatizadas em estufas. A determinação do nível de ploidia das plantas foi realizada por citometria de fluxo. A colchicina demonstrou ser tóxica aos explantes das três variedades, ocasionando redução significativa no número médio de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Tetraplóide; Epicótilo; Regeneração in vitro; Cultura de tecidos. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007001000009 |
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| Machado,Marcos A.; Cristofani-Yaly,Mariângela; Bastianel,Marinês. |
| Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture regarding the phytosanitary problems. A higher number of varieties/genotypes with potential for commercial growing, either for the industry or fresh market, has been one of the main objectives of citrus breeding programs. The genetic breeding of citrus has improved, in the last decades, due to the possibility of an association between biotechnological tools and classical methods of breeding. The use of molecular markers for early selection of zygotic seedlings from controlled crosses resulted in the possibility of selection of a high number of new combination and, as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citriculture; Improvement; Hybrids. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000500019 |
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| Pacheco,Camilla de Andrade; Schinor,Evandro Henrique; Azevedo,Fernando Alves de; Bastianel,Marinês; Cristofani-Yaly,Mariângela. |
| Desde 1997, o Centro APTA Citros Sylvio Moreira/IAC conduz um amplo programa de melhoramento genético de citros via cruzamentos dirigidos. Destes cruzamentos, alguns híbridos de tangerina com laranja vêm sendo selecionados para qualidade de fruta e potenciais novas variedades. Com isso, frutos de um híbrido entre tangor Murcott (TM) e laranja Pera (LP), denominado TMxLP 290, foram submetidos a análises físico-químicas e teste sensorial de aceitabilidade, a fim de avaliar a aceitação de tal variedade pelo mercado consumidor. Foram analisados os parâmetros físico-químicos: sólidos solúveis, acidez total titulável, ratio, rendimento de suco, índice de cor, número de sementes e massa do fruto. Aliado a essas análises, realizou-se o teste sensorial de aceitação... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Análise sensorial; Teste de aceitação do consumidor; Pós-colheita de citros. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452014000400006 |
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