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Development and application of qRT-PCR for sugar beet gene expression analysis in response to in vitro induced water deficit Electron. J. Biotechnol.
Taski-Ajdukovic,Ksenija; Nagl,Nevena; Kovacev,Lazar; Curcic,Zivko; Danojevic,Dario.
Sugar beet is a significant industrial crop, often grown in the areas where summer drought can severely limit root yield and sugar content. In order to improve development of sugar beet cultivars with increased drought tolerance it is necessary to understand plant response to water stress at the genomic level. Since recent research efforts have focused on the molecular response of the plant in order to identify water deficit inducible genes, the aim of this investigation was to develop qRT-PCR methodology for the quantification of gene expression in sugar beet under conditions of water deficiency in vitro. Sugar beet genotypes, selected for different response to water deficit, were grown and multiplied in vitro. Axilary shoots were placed on...
Tipo: Journal article Palavras-chave: Beta vulgaris; Drought; In vitro; Real-time PCR.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600010
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