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| Mejlholm, Ole; Gunvig, Annemarie; Borggaard, Claus; Blom-hanssen, Jesper; Mellefont, Lyndal; Ross, Tom; Leroi, Francoise; Else, Tony; Visser, Diana; Dalgaard, Paw. |
| The performance of six predictive models for Listeria monocytogenes was evaluated using 1014 growth responses of the pathogen in meat, seafood, poultry and dairy products. The performance of the growth models was closely related to their complexity i.e. the number of environmental parameters they take into account. The most complex model included the effect of nine environmental parameters and it performed better than the other less complex models both for prediction of maximum specific growth rates (mu(max) values) and for the growth boundary of L. monocytogenes. For this model bias and accuracy factors for growth rate predictions were 1.0 and 1.5, respectively, and 89% of the growth/no-growth responses were correctly predicted. The performance of three... |
| Tipo: Text |
Palavras-chave: Predictive models; Bias and accuracy factors; Correct prediction percentage; Growth/no-growth predictions; Psi (psi) value. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12218/11422.pdf |
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| Mace, Sabrina; Mamlouk, Kelthoum; Chipchakova, Stoyka; Prevost, Herve; Joffraud, Jean-jacques; Dalgaard, Paw; Pilet, Marie-france; Dousset, Xavier. |
| A specific real-time PCR quantification method combined with a propidium monoazide sample treatment step was developed to determine quantitatively the viable population of the Photobacterium phosphoreum species group in raw modified-atmosphere-packed salmon. Primers were designed to amplify a 350-bp fragment of the gyrase subunit B gene (gyrB) of P. phosphoreum. The specificity of the two primers was demonstrated by using purified DNA from 81 strains of 52 different bacterial species. When these primers were used for real-time PCR in pure culture, a good correlation (R-2 of 0.99) was obtained between this method and conventional enumeration on marine agar (MA). Quantification was linear over 5 log units as confirmed by using inoculated salmon samples. On... |
| Tipo: Text |
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| Ano: 2013 |
URL: https://archimer.ifremer.fr/doc/00135/24623/22772.pdf |
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| Podeur, Gaetan; Dalgaard, Paw; Leroi, Francoise; Prevost, Herve; Ernborg, Jette; Martinussen, Jan; Hansen, Lars Hestbjerg; Filet, Marie-france. |
| Histamine fish poisoning is common and due to toxic concentrations of histamine often produced by Gram-negative bacteria in fin-fish products with a high content of the free amino acid histidine. The genus Morganella includes two species previously reported to cause incidents of histamine fish poisoning. Morganella morganii and Morganella psychrotolerans are both strong producer of histamine. However, little is known about the occurrence and critical stages for fish contamination with these bacteria. To elucidate contamination routes of Morganella, specific real-time quantitative PCR (RTi qPCR) methods for quantification of M. morganii and M. psychrotolerans have been developed. Selective primers amplified a 110 bp region of the vasD gene for M.... |
| Tipo: Text |
Palavras-chave: Histamine-producing bacteria; Tuna; Selective medium; Enterobacteriaceae; Galactokinase; Type VI secretion system. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00254/36551/35096.pdf |
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