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	Provedor de dados Genet. Mol. Biol. (3) Rev. Bras. entomol. (1) Autor Deprá,Maríndia (4) Zanini,Rebeca (2) Diesel,José F. (1) D’Ávila,Marícia Fantinel (1) Loreto,Elgion L.S. (1) Loreto,Élgion Lucio da Silva (1) Panzera,Yanina (1) Sepel,Lenira Maria Nunes (1) Valente,Vera Lúcia da Silva (1) Valente-Gaiesky,Vera Lucia S. (1) Vieira,Gilberto Cavalheiro (1) da Silva Valente,Vera Lúcia (1) Mais... Palavra-chave Drosophila (2) Transposable elements (2) Cis-regulatory sequences (1) Cryptic species (1) Dnmt2 (1) Drosophila paulistorum complex (1) Drosophila willistoni subgroup (1) Epigenetic (1) GFP (1) Hobo (1) Male genitalia (1) PiggyBac (1) Positive selection (1) Positive-destabilizing selection (1) Semispecies (1) Transgenic Drosophila (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2018 (2) 2015 (1) 2004 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers Genet. Mol. Biol. Deprá,Maríndia; Sepel,Lenira Maria Nunes; Loreto,Élgion Lucio da Silva. We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation. Tipo: Info:eu-repo/semantics/article Palavras-chave: Transgenic Drosophila; PiggyBac; GFP; Transposable elements. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012 Drosophila relics hobo and hobo-MITEs transposons as raw material for new regulatory networks Genet. Mol. Biol. Loreto,Elgion L.S.; Deprá,Maríndia; Diesel,José F.; Panzera,Yanina; Valente-Gaiesky,Vera Lucia S.. Abstract Hypermutable strains of Drosophila simulans have been studied for 20 years. Several mutants were isolated and characterized, some of which had phenotypes associated with alteration in development; for example, showing ectopic legs with eyes being expressed in place of antennae. The causal agent of this hypermutability is a non-autonomous hobo-related sequence (hoboVA). Around 100 mobilizable copies of this element are present in the D. simulans genome, and these are likely mobilized by the autonomous and canonical hobo element. We have shown that hoboVA has transcription factor binding sites for the developmental genes, hunchback and even-skipped, and that this transposon is expressed in embryos, following the patterns of these genes. We suggest... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hobo; Transposable elements; Cis-regulatory sequences; Drosophila. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200198 Evolution of DNMT2 in drosophilids: Evidence for positive and purifying selection and insights into new protein (pathways) interactions Genet. Mol. Biol. Vieira,Gilberto Cavalheiro; D’Ávila,Marícia Fantinel; Zanini,Rebeca; Deprá,Maríndia; da Silva Valente,Vera Lúcia. Abstract The DNA methyltransferase 2 (DNMT2) protein is the most conserved member of the DNA methyltransferase family. Nevertheless, its substrate specificity is still controversial and elusive. The genomic role and determinants of DNA methylation are poorly understood in invertebrates, and several mechanisms and associations are suggested. In Drosophila, the only known DNMT gene is Dnmt2. Here we present our findings from a wide search for Dnmt2 homologs in 68 species of Drosophilidae. We investigated its molecular evolution, and in our phylogenetic analyses the main clades of Drosophilidae species were recovered. We tested whether the Dnmt2 has evolved neutrally or under positive selection along the subgenera Drosophila and Sophophora and investigated... Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila; Dnmt2; Positive selection; Epigenetic; Positive-destabilizing selection. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200215 Can sibling species of the Drosophila willistoni subgroup be recognized through combined microscopy techniques? Rev. Bras. entomol. Zanini,Rebeca; Deprá,Maríndia; Valente,Vera Lúcia da Silva. ABSTRACT In several arthropod groups, male genitalia is the most important feature for species identification, especially in cryptic species. Cryptic species are very common in the Drosophila genus, and the Neotropical Drosophila willistoni species group is a good example. This group currently includes 24 species divided into three subgroups: alagitans, bocainensis and willistoni. There are six sibling species in the willistoni subgroup – D. willistoni, D. insularis, D. tropicalis, D. equinoxialis, D. pavlovskiana and D. paulistorum, which is a species complex composed of six semispecies – Amazonian, Andean-Brazilian, Centroamerican, Interior, Orinocan and Transitional. The objective of this study was to characterize male genitalia of the willistoni... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptic species; Drosophila willistoni subgroup; Drosophila paulistorum complex; Male genitalia; Semispecies. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262015000400323 Registros recuperados: 4 Primeira ... 1 ... Última

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