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| Viancelli,Aline; Kunz,Airton; Esteves,Paulo Augusto; Bauermann,Fernando Viçosa; Furukawa,Kenji; Fujii,Takao; Antônio,Regina Vasconcellos; Vanotti,Matias. |
| The present study aimed to describe the bacterial community present at an anaerobic up flow bioreactor with ANAMMOX activity, inoculated with the sludge from the anaerobic pond of a swine slurry treatment system. The description was based on the molecular DNA techniques using primers for amplification of complete 16S rRNA gene and also new primers to amplify smaller fragments from 16S rRNA. During the bioreactor operation time, the bacterial community changed significantly, increasing the nitrogen removal efficiency, reaching after 500 days a removal rate of 94%. The complete PCR amplification of 16S rRNA gene generated 17 clones, where three presented similarity with Candidatus Jettenia asiatica (97%), twelve with Janthinobacterium (99%) and two with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ANAMMOX; Anaerobic pond; Sequencing; Swine manure. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500022 |
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| Dambros,Régia Maria Feltrin; Ribeiro,Bergman Moraes; Aguiar,Raimundo Wagner de S.; Schaefer,Rejane; Esteves,Paulo Augusto; Perecmanis,Simone; Simon,Neide Lisiane; Silva,Nayara Cavalcante; Coldebella,Michele; Ciacci-Zanella,Janice Reis. |
| Aujeszky' s disease (AD) is an infectious disease causing important economic losses to the swine industry worldwide. The disease is caused by an alpha-herpesvirus, Aujeszky' s disease virus (ADV), an enveloped virus with a double stranded linear DNA genome. The ADV genome encodes 11 glycoproteins, which are major targets for the immune system of the host in response to the infection. The glycoprotein E (gE) is a non-essential protein and deletion of the gE gene has been used for the production of marker vaccines. Aiming to develop molecular reagents for the production of a gE specific ELISA test, the gE gene was amplified by PCR, cloned and expressed into a baculovirus expression system. The recombinant DNA vector pFastBac-gE-ADV was used for site-specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aujeszky; Glycoprotein E; Baculovírus; Recombinant. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300021 |
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| Munhoz,Lívia Silveira; Vargas,Gilberto D'Ávila; Fischer,Geferson; Lima,Marcelo de; Esteves,Paulo Augusto; Hübner,Silvia de Oliveira. |
| Immunoglobulin Y (IgY) is the major antibody isotype in birds, reptiles, amphibia, and lungfish, playing a similar biological role as mammal IgG. Due to its phylogenetic distance, immune diversification and presence in the egg yolk, IgY provide a number of advantages in immunodiagnostic compared to IgG from mammals. Moreover, IgY production is in agreement with international efforts to reduce, refine and if possible, to replace animals in experimentation, contributing substantially in favor of animal welfare. This article presents an overview about structural and functional features, production and applications of IgY in immunodiagnostic, as well as the advantages of chicken antibodies use. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Egg yolk antibody; Immunodiagnostics; Immunoglobulin Y; Polyclonal antibodies. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000100025 |
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