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The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells Genet. Mol. Biol.
Mansouri,Vahid; Salehi,Mohammad; Nourozian,Mohsen; Fadaei,Fatemeh; Farahani,Reza Mastery; Piryaei,Abbas; Delbari,Ali.
Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differentiation; Germ cells; Nuclear transfer embryonic stem cells.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200220
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Corpus Callosum Size, is There a Sexual Difference? International Journal of Morphology
Yasin,Mohammad; Farahani,Reza Mastery.
Physical anthropometry is a subdivision of human anatomy science, which has uses in medical industries. A lot of studies showed that genetic, racial, and socioeconomic factors and educational background play rule in anthropometry. We aim this study to determine the presence of differences between gender and corpus callosum size. MRIs were collected from Ardebil and Kermanshah states done in 2013, the participants were informed about this study. They were selected based on age more than 20-years old, absence of demyelization and degenerative diseases, clean history of for neurosurgery, and previous cerebrovascular accidents. MRIs were analyzed by PmsDview program in the midsagittal section by using 9 landmarks, and the data was analyzed by SPSS 19.0. The...
Tipo: Journal article Palavras-chave: Corpus callosum; Sex-related size; Sex-related; Anthropometry; Iran; MRI; Sexual dimorphism.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100016
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