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Registros recuperados: 14
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Growth capacity of thermotolerant campylobacters in culture media supplemented with pig and cow blood BABT
Tresierra-Ayala,Álvaro; Navas,Manuel; Flores,Josué; Perea,Ramsés; Huanaquiri,Juan; Bendayán,María; Fernández,Heriberto.
In this work 60 thermotolerant Campylobacter strains (37 C. jejuni and 23 C. coli) isolated from the cows, pigs, chickens and ducks (15 strains of each type of animal) were used to establish their growth capacity on media containing cow or swine blood as potential substitutes of sheep or horse blood. The growth capacity was assessed by viable counts on cow and swine blood media, using the modified Miles and Misra method. Campylobacter strains showed better growth in the media supplemented with pig or sheep blood than with cow blood. Thus, the use of pig blood could be a supplement for Campylobacter culture medium, when there was no availability of sheep or horse blood.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter; Culture media; Cow blood; Swine blood.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500011
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Susceptibility of Arcobacter butzleri to heavy metals BJM
Otth,Laura; Solís,Gabriela; Wilson,Myra; Fernández,Heriberto.
The susceptibility of 50 strains of Arcobacter butzleri isolated from chicken liver [12], mussels [18], river water [6] and bovine [5], duck [2] and pelicans [7] feces to mercury (Hg), chromium (Cr), silver (Ag), nickel (Ni), cobalt (Co), iron (Fe), manganese (Mn), molybdenum (Mo) and lead (Pb) was determined.All the strains were resistant to Mo, Mn, Ni, Co, Pb and Fe and susceptible to Hg, Ag and Cr. MIC values showed high variability, indicating a non homogeneous behavior among the strains.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arcobacter butzleri; Heavy metals; Resistance.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300015
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Campylobacter insulaenigrae: first isolation report from South American sea lion (Otaria flavescens, (Shaw, 1800) BJM
González,Mario; Villanueva,Maria Paz; Debruyne,Lies; Vandamme,Peter; Fernández,Heriberto.
Campylobacter insulaenigrae have been isolated from different pinnipeds but not from South American sea lion (Otaria flavescens). The aim of this work is to report the first isolation of C. insulaenigrae from South American sea lion (Otaria flavescens). The isolate, identified by its phenotypic and molecular characteristics, allow recognizing O. flavescens as a new host for C. insulaenigrae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter insulaenigrae; Otaria flavescens; South American sea lion; Reservoir.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100033
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Isolation of Helicobacter pylori in gastric mucosa and susceptibility to five antimicrobial drugs in Southern Chile BJM
Otth,Laura; Wilson,Myra; Fernández,Heriberto; Otth,Carola; Toledo,Claudio; Cárcamo,Victoria; Rivera,Paula; Ruiz,Luis.
Helicobacter pylori colonizes more than 50% of the world population thus, it is considered an important cause of gastric cancer. The aim of this study was to determine the isolation frequency of H. pylori in Southern Chile from patients with symptomatology compatible with gastritis or gastric ulcer and to correlate these findings with demographic parameters of infected patients and the susceptibility profiles of the isolated strains to the antimicrobial drugs used in the eradication treatments. A total of 240 patients were enrolled in the study. Each gastric biopsy was homogenized and seeded onto blood agar plates containing a selective antibiotics mixture (DENT supplement). Plates were incubated at 37° C in a microaerophilic environment for five days. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helicobacter pylori; Antimicrobial susceptibility; Gastric ulcer.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200005
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Neonatal Campylobacter coli hemorrhagic enteritis and bacteraemia BJM
Ruiz-Esquide,Fernando; Lafourcade,Mónica; Andrews,Edilia; Fernández,Heriberto.
A case of neonatal campylobacteriosis with hemorrhagic enteritis and bacteraemia due to Campylobacter coli is presented. The mother, from a rural area, had three febrile self-limited diarrheic episodes during pregnancy. The neonate probably acquired the infection during labor. The newborn's serum showed high levels of specific immunoglobulins which could explain the scarce symptoms in this newborn, despite the delay in establishing the gentamicin therapy.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Campylobacter coli; Campylobacteriosis; Hemorrhagic enteritis; Bacteraemia; Newborn.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400011
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Occurrence of Campylobacter jejuni and Campylobacter coli and their biotypes in beef and dairy cattle from the south of Chile BJM
Fernández,Heriberto; Hitschfeld,Marianne.
The prevalence of Campylobacter jejuni and Campylobacter coli and their biotypes in beef and dairy cattle from the South of Chile was established. Campylobacter were statistically more prevalent among beef cattle (35.9%) than among dairy cattle (21.3%), being C. jejuni the species most frequently isolated.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter jejuni; C. coli; Beef cattle; Dairy cattle.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300005
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Campylobacter jejuni occurrence in chicken fecal samples from small properties in Pelotas, southern of Brazil BJM
Gomes,Fabiane R.; Curcio,Bruna R.; Ladeira,Sílvia R.L.; Fernández,Heriberto; Meireles,Mário Carlos Araújo.
The aim of this study was to evaluate the occurrence of thermophilic Campylobacter species in broiler flocks from 26 small properties located near the campus of Federal University of Pelotas, RS, Brazil. A total of 404 chicken fecal samples were analyzed and after isolation, identification and biotyping was performed according to Lior´s scheme. Twenty one strains (5.2%) of Campylobacter jejuni biotype II were isolated from animals from seven properties (26.9%), with a variation from 3% to 34.6% among the positive properties. We also analyzed twelve C. jejuni isolates by polymerase chain reaction (PCR), using specific primers that generate a fragment of 402 pb. Our study allowed us to observe that chickens raised in non-industrial establishments without...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter jejuni; Thermotolerant species; Diarrhea; Chicken.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300032
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Desiccation resistance in Arcobacter butzleri BJM
Otth,Laura; Wilson,Myra; Fernández,Heriberto.
The desiccation resistance of A. butzleri was studied. Two, 3 and 4 of the strains did not resist desiccation for more than 2, 12 and 36 h, respectively. Two strains resisted desiccation for > 48 h. A. butzleri seems to be more resistant to desiccation than the classical enteropathogenic Campylobacter species.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Arcobacter butzleri; Campylobacteraceae; Desiccation resistance.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400011
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Subtyping of Chilean Methicillin-Resistant Staphylococcus aureus strains carrying the staphylococcal cassette chromosome mec type I BJM
Medina,Gustavo; Otth,Carola; Otth,Laura; Fernández,Heriberto; Muñoz,Celeste; Cruz,María; Zaror,Ángela; Henriquez,Ruby; Arce,Maria; Wilson,Myra.
The cassette chromosome mec (SCCmec) present in methicillin-resistant Staphylococcus aureus (MRSA) has two essential components, the ccr gene complex and the mec gene complex. Additionally, SCCmec has non-essential components called J regions which are used for MRSA subtyping. This study was performed to determine subtypes MRSA strains carrying SCCmec type I based on polymorphism of regions located downstream of the mecA gene. A total of 98 MRSA strains carrying SCCmec type I isolated from patients hospitalized at the County Hospital of Valdivia (Chile) between May 2007 and May 2008, were analyzed by multiplex PCR designed to amplify the mecA gene and 7 DNA hypervariable regions located around the mecA gene. MRSA strains were classified into seventeen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Subtypified MRSA; Polymorphism MRSA; SCCmec MRSA.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300006
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Adhesive and invasive capacities of Edwarsiella tarda isolated from South American sea lion BJM
Fernández,Araceli; Paz Villanueva,María; González,Mario; Fernández,Fabiola; Latif,Fadua; Flores,Sandra Nonier; Fernández,Heriberto.
Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and invasive ability of 17 E. tarda strains isolated from SASL fecal material. All the strains were able to adhere and invade HEp-2 cells with adhesion and invasion percentages ranging from 56 to 100% and 21 to 74%, respectively. Despite the expression of these pathogenic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Edwarsiella tarda; Adherence; Invasion; Otaria flavescens.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300044
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Occurrence of campylobacter species in healthy well-nourished and malnourished children BJM
Fernández,Heriberto; Vera,Fernando; Villanueva,María Paz; García,Andrea.
The occurrence of Campylobacter species in healthy, well-nourished and healthy, malnourished children of low socioeconomic level in Southern Chile was determined. Campylobacter carriers were significantly most frequent among malnourished (31.4%) than among well-nourished (9.9%) children. Six species were isolated from malnourished children whereas four were found among well-nourished children. C. upsaliensis was the most frequent (13.3%) species isolated from malnourished children, followed by C. lari (7.6%) and C. fetus ssp. fetus (1.9%).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter species; Malnourished children; Well-nourished children; Healthy carriers.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100013
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Arcobacter butzleri an emerging enteropathogen: communication of two cases with chronic diarrhea BJM
Fernández,Heriberto; Krause,Sergio; Paz Villanueva,María.
The first two cases of chronic diarrhea due to Arcobacter butzleri in Chile are reported. The clinical findings, the absence of other enteropathogens, virus or parasites, the epidemiological association between both patients, the treatment outcome and the fact that A. butzleri was the only bacteria isolated, support the assumption that it was the etiological agent of these chronic diarrhea cases.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arcobacter butzleri; Chronic diarrhea; Emerging pathogen.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200008
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A case of acute diarrhea due to the emerging pathogen Campylobacter jejuni subsp. doylei in Southern Chile BJM
Fernández,Heriberto; Rodríguez,Roxana; Barudi,Carmen; Lobos,Marta.
The first documented case of acute diarrhea due to C. jejuni subsp. doylei in Chile is reported. The clinical findings, the absence of other enteropathogens, virus or parasites and the fact that C. jejuni subsp. doylei was the only bacteria isolated support the assumption that it was the etiological agent of this diarrheal case.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter jejuni subsp. doylei; Enteritis; Emerging pathogen.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100011
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Occurrence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from domestic animals from Southern Ecuador Ciência Rural
Toledo,Zorayda; Simaluiza,Rosa Janneth; Fernández,Heriberto.
ABSTRACT: Occurrence and antimicrobial resistance profiles of Campylobacter jejuni and C. coli strains isolated from fecal samples of dogs, pigs, cows and hens in southern Ecuador were studied. Of the 250 samples studied, 84 (33.6%) were Campylobacter positive, with C. jejuni being more frequent (78.6%) than C. coli (21.4%), with the exception of porcine samples, from which C. coli was the only species isolated. Multidrug resistance was reported in 10 Campylobacter strains (11.9%), four C. jejuni and six C. coli, and was always associated with nalidixic acid and ciprofloxacin resistance. All of the amoxicillin-resistant strains were susceptible to amoxicillin-clavulanic acid and were therefore beta-lactamase producers. However, one strain of C. jejuni...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter; Reservoirs; Domestic animals; Antimicrobial resistance.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100450
Registros recuperados: 14
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