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Natural abundance of N-15 and C-13 in fish tissues and the use of stable isotopes as dietary protein tracers in rainbow trout and gilthead sea bream ArchiMer
Beltran, M.; Fernandez-borras, J.; Medale, Francoise; Perez-sanchez, J.; Kaushik, Sadasivam; Blasco, J..
For developing efficient diets, two sets of experiments examined whether the use and allocation of dietary protein can be traced by labelling with stable isotopes (N-15 and C-13) in two culture fish (Oncorhynchus mykiss and Sparus aurata). In the first experiment, natural abundance and tissue distribution of these isotopes were determined, by measuring the delta C-13 and delta N-15 values by isotopic ratio mass spectrometry, in fingerlings (14-17 g) adapted to diets differing in the percentage of fish meal replacement by plant protein sources. For both species, delta N-15 and delta C-13 were greater in tissues with higher protein and lower lipid content. Delta N-15 of diets and tissues decreased as replacement increased, suggesting delta N-15 can be used...
Tipo: Text Palavras-chave: Sea bream; Rainbow trout; Protein; Diet; Delta N 15; Delta C 13.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6966.pdf
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