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Bioconversion of (+)- and (-)-alpha-pinene to (+)- and (-)-verbenone by plant cell cultures of Psychotria brachyceras and Rauvolfia sellowii Electron. J. Biotechnol.
Limberger,Renata Pereira; Aleixo,Adriana Mendes; Fett-Neto,Arthur Germano; Henriques,Amélia T.
This work describes the bioconversion of (-)- and (+)-alpha-pinene (2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene), targeted at the production of (-)- and (+)-verbenone (4,6,6-trimethyl-bicyclo (3.1.1) hept-3-en-2-one), respectively, using Psychotria brachyceras and Rauvolfia sellowii cell suspension cultures. P. brachyceras showed selectivity to (-)-alpha-pinene with 80.9% conversion (relative integrated area gas chromatography-mass spectrometry (GC-MS)) of (-)-verbenone in 10-day-incubation, whereas R. sellowii was able to convert both pinene enantiomers (37.6% conversion of (-)-verbenone in 7-day-incubation and 32.2% conversion of (+)-verbenone in 10-day-incubation). In both systems trans-verbenol was formed as main product and then slowly biocatalyzed to...
Tipo: Journal article Palavras-chave: Alpha-pinene; Biotransformation; Verbenol; Verbenone.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400003
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Iron homeostasis related genes in rice Genet. Mol. Biol.
Gross,Jeferson; Stein,Ricardo José; Fett-Neto,Arthur Germano; Fett,Janette Palma.
Iron is essential for plants. However, excess iron is toxic, leading to oxidative stress and decreased productivity. Therefore, plants must use finely tuned mechanisms to keep iron homeostasis in each of their organs, tissues, cells and organelles. A few of the genes involved in iron homeostasis in plants have been identified recently, and we used some of their protein sequences as queries to look for corresponding genes in the rice (Oryza sativa) genome. We have assigned possible functions to thirty-nine new rice genes. Together with four previously reported sequences, we analyzed a total of forty-three genes belonging to five known protein families: eighteen YS (Yellow Stripe), two FRO (Fe3+-chelate reductase oxidase), thirteen ZIP (Zinc regulated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron; Rice; Genome analysis; Iron transporters; Ferritin.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400012
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Sustainable production of bioactive alkaloids in Psychotria L. of southern Brazil: propagation and elicitation strategies Acta Botanica
Magedans,Yve Verônica da Silva; Rodrigues-Corrêa,Kelly Cristine da Silva; Costa,Cibele Tesser da; Matsuura,Hélio Nitta; Fett-Neto,Arthur Germano.
ABSTRACT Psychotria is the largest genus in Rubiaceae. South American species of the genus are promising sources of natural products, mostly due to bioactive monoterpene indole alkaloids they accumulate. These alkaloids can have analgesic, antimutagenic, and antioxidant activities in different experimental models, among other pharmacological properties of interest. Propagation of genotypes with relevant pharmaceutical interest is important for obtaining natural products in a sustainable and standardized fashion. Besides the clonal propagation of elite individuals, the alkaloid content of Psychotria spp. can also be increased by applying moderate stressors or stress-signaling molecules. This review explores advances in research on methods for plant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Alkaloids; Elicitation; Monoterpenes; Plant propagation; Psychotria; Southern Brazil; Sustainability.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000400607
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