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	Provedor de dados Biological Sciences (2) Autor Fraga, Hugo Pacheco de Freitas (2) Guerra, Miguel Pedro (2) Almeida, Vivian (1) Navarro, Bruno Bachiega (1) Pavei, Ana Flávia (1) Pescador, Rosete (1) Vieira, Leila do Nascimento (1) Mais... Palavra-chave Micropropagation (2) 2.03.00.00-0 (1) 2.03.00.00-0 Botânica in vitro culture (1) 2.03.03.00-9 (1) 2.03.06.00-8 orchids (1) Epigenetics (1) Feijoa. 2.03.06.00-8 Botânica Aplicada (1) In vitro culture (1) PLBs. Botânica (1) Somatic embryo conversion (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2017 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Effects of glutathione supplementation and carbon source during somatic embryogenesis of Acca sellowiana (O.Berg) Burret (Myrtaceae) Biological Sciences Pavei, Ana Flávia; Fraga, Hugo Pacheco de Freitas; Vieira, Leila do Nascimento; Guerra, Miguel Pedro.  The present study intended to investigate the effects of different glutathione (GSH) levels (0, 0.1, 0.5 and 1 mM) on the somatic embryogenesis (SE) induction of Acca sellowiana. Besides, we evaluated the effect of different carbon sources (sucrose and maltose) on the somatic embryos conversion. GSH-supplemented treatments resulted in improved SE induction rates (~70%) as compared to the control GSH-free (~35%) after 50 days of culture. The total number of somatic embryos obtained did not differ between treatments, but significant differences were observed for the embryonic stages after 80 days of culture. After 80 days of culture, 0.5 and 1 mM GSH-supplemented treatments showed the largest amount of torpedo-staged somatic embryos. In contrast, treatments... Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.00.00-0 Botânica in vitro culture; Micropropagation; Somatic embryo conversion; Feijoa. 2.03.06.00-8 Botânica Aplicada. Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40257 Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of Cattleya tigrina A. Richard Biological Sciences Almeida, Vivian; Fraga, Hugo Pacheco de Freitas; Navarro, Bruno Bachiega; Guerra, Miguel Pedro; Pescador, Rosete. Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in... Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.00.00-0; 2.03.03.00-9; 2.03.06.00-8 orchids; Micropropagation; In vitro culture; Epigenetics; PLBs. Botânica. Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36656 Registros recuperados: 2 Primeira ... 1 ... Última

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