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| Beloti,Vanerli; Barros,Márcia A.F.; Freitas,Júlio C. de; Nero,Luís A.; Souza,Juliana A. de; Santana,Elsa H.W.; Franco,Bernadette. D.G.M.. |
| 2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration of microbial colonies in solid culture media, being a key component of the dry rehydratable film system used for microbiological analysis of food. This dye is colorless in the oxidized form and red when reduced by microorganisms, due to formation of formazan. In this study, TTC was added to Plate Count Agar (PCA) for enumeration of microorganisms in thirty four pasteurized milk samples, with the aim to verify the frequency of microorganisms that are unable to reduce TTC. Milk samples were decimally diluted in saline and pour-plated in PCA plus 0.015% TTC. Colonies were counted after 24h and 48 h of incubation at 35oC. From a total of 50,574 colonies, 19,665 (38.88%) did not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2; 3; 5-triphenyltetrazolium chloride TTC pasteurized milk microbial counts. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200009 |
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| Vidotto,Marilda C.; Lima,Natália C.S. de; Fritzen,Juliana T.T.; Freitas,Júlio C. de; Venâncio,Emerson J.; Ono,Mario A.. |
| Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genes detected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) and STx2e (3%). Twenty... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Porcine Escherichia coli; Virulence genes; Postweaning diarrhea; Toxins. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100035 |
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