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An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica ArchiMer
Lechable, Marion; Jan, Anne-cecile; Duchene, Axel; Uveira, Julie; Weissbourd, Brandon; Gissat, Loann; Collet, Sophie; Gilletta, Laurent; Chevalier, Sandra; Leclère, Lucas; Peron, Sophie; Barreau, Carine; Lasbleiz, Régis; Houliston, Evelyn; Momose, Tsuyoshi.
The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different...
Tipo: Text Palavras-chave: Animal culture; Cnidarian; Developmental biology; Genetics; Jellyfish.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00656/76820/77996.pdf
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First records of dusky grouper Epinephelus marginatus settlement-stage larvae in the Ligurian Sea ArchiMer
Faillettaz, Robin; Gilletta, Laurent; Petit, Franck; Francour, Patrice; Irisson, Jean-olivier.
The presence of dusky grouper Epinephelus marginatus settlement-stage larvae in the Ligurian Sea is reported. Epinephelus marginatus populations are slowly recovering along the french Mediterranean coast since the establishment of a moratorium on spearfishing in 1993 but still remain spatially restricted to marine protected areas. The occurrence of Epinephelus marginatus larvae in a region where effective settlement had not been detected for decades is a new observation supporting the species’ recovery.
Tipo: Text Palavras-chave: Epinephelus marginatus; Epinephelidae; Settlement-stage larvae; Ligurian Sea; NW Mediterranean Sea..
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00634/74622/74514.pdf
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