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| Souza,Vanessa Ribeiro de; Silva,Ana C. G.; Pinotti,Laura Marina; Araújo,Heloísa Sobreiro Selistre; Giordano,Raquel de Lima Camargo. |
| The purpose of this work was to characterize the enzyme penicillin G acylase (PGA) produced by Bacillus megaterium. Purification of the enzyme by ultra/diafiltration did not allow the detection of the PGA band by SDS-PAGE electrophoresis due to the high content of remaining proteins. However, using the DNA of the microorganism, it was possible to replicate the genes of the two B. megaterium PGA reported in literature, showing that the enzyme consisted of two sub-units, having 245 and 537 amino acids each and an average molecular mass of 26950 and 59070 Da, respectively. The parameters studied were: 1) the influence of temperature in the 25-60(0)C range, 2) pH in the 5-10 range and 3) substrate concentration, this was tested to obtain results on the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Penicillin G acylase; Bacillus megaterium; Characterization. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400013 |
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| Suarez,Carlos Alberto Galeano; Montano,Inti Doraci Cavalcanti; Nucci,Edson Romano; Iemma,Monica Rosas da Costa; Giordano,Raquel de Lima Camargo; Giordano,Roberto de Campos. |
| Bacillus megaterium is a promising host for expression of heterologous proteins. This paper reports the nutrient consumption patterns and production of metabolites for three different strains of B. megaterium, ATCC 14945, QMB 1551 and PV 361, which is QMB 1551 with seven constitutive plasmids deleted. 14 h cultivations in agitated flasks were run, for two different media: A (LB plus 10g/L glucose) and B (medium A, with the yeast extract replaced by tryptone). Strains PV361 and QMB 1551 showed higher maximum specific growth rates in medium B, reaching 0.42 h-1 and 0.48 h-1 respectively. The main by-products of the glucose overflow mechanism were acetate and lactate, for all three strains, which had preferential amino acids for substrate: Ala, Asp, Glu, Ser.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metabolic profile; Bacillus megaterium; Culture media. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400001 |
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