|
|
|
|
|
Corredor, Jenny; De Conti, Daniela; Guerra, Miguel Pedro; Dal Vesco, Lirio Luiz; Pescador, Rosete. |
Tissue culture techniques have been employed for bromeliad mass propagation by means of the morphogenetic route of nodular cluster cultures (NCs). This study aimed to assess protein, carbohydrate and global DNA methylation (GDM) level dynamics during NCs induction from Vriesea reitzii seeds. Seeds were inoculated into Murashige and Skoog (MS) liquid medium supplemented with 4 µM α-naphthaleneacetic acid (NAA) to induce NCs and in culture medium without plant growth regulators to form normal seedlings. Samples collected at 0, 3, 7, 10, 14, and 21 days of culture were analyzed. All parameters assessed showed the same variation pattern. However, seeds inducing NCs showed significantly lower starch (6.0 mg g-1 FM), carbohydrate (10.7 mg g-1 FM) and GDM (11.0%)... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bromeliaceae; Bromeliad; Germination; Micropropagation; Seedling. Bromeliaceae bromeliad germination micropropagation seedling.. |
Ano: 2020 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42448 |
| |
|
|
De Conti, Daniela; Corredor-Prado, Jenny Paola; Roecker Junior, Djalma; Suzuki, Rogério Mamoru; Guerra, Miguel Pedro; Pescador, Rosete. |
Tissue culture techniques have been employed for orchid mass propagation by means of the morphogenetic route of protocorm like-bodies (PLBs). This study aimed to analyze and compare Indole-acetic acid (IAA) and sugar endogenous levels in protocorm-like bodies (PLBs) induction and development in Cattleya tigrina, in order to better understand this process and to optimize micropropagation procedure protocols. Leaves grown on MS (Murashige and Skoog) culture medium, suplemented with 9 μM TDZ for PLBs induction and development were collected after 0, 2, 7, 14, 30, 60 and 100 days of cultivation, for further analysis. Increase of IAA and reduction of sugar levels are strongly related to morphogenetic response, that is, PLBs formation over the preexisting ones... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orchid; Hormones; Sugars; Morphogenesis.. |
Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37874 |
| |
|
|
Pavei, Ana Flávia; Fraga, Hugo Pacheco de Freitas; Vieira, Leila do Nascimento; Guerra, Miguel Pedro. |
The present study intended to investigate the effects of different glutathione (GSH) levels (0, 0.1, 0.5 and 1 mM) on the somatic embryogenesis (SE) induction of Acca sellowiana. Besides, we evaluated the effect of different carbon sources (sucrose and maltose) on the somatic embryos conversion. GSH-supplemented treatments resulted in improved SE induction rates (~70%) as compared to the control GSH-free (~35%) after 50 days of culture. The total number of somatic embryos obtained did not differ between treatments, but significant differences were observed for the embryonic stages after 80 days of culture. After 80 days of culture, 0.5 and 1 mM GSH-supplemented treatments showed the largest amount of torpedo-staged somatic embryos. In contrast, treatments... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.03.00.00-0 Botânica in vitro culture; Micropropagation; Somatic embryo conversion; Feijoa. 2.03.06.00-8 Botânica Aplicada. |
Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40257 |
| |
|
| |
|
|
|