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| Góis,Marcelo Biondaro; Hermes-Uliana,Catchia; Paltanin,Alessandra; Pontes,Willian; Araújo,Eduardo José De Almeida; Miranda Neto,Marcilio Hubner; Sant'Ana,Débora Mello Gonçales. |
| Enteric nervous plexuses have been the object of several studies, specially the myenteric plexus whose studies describe its organization, functions and alterations. On the other hand, the submucosal plexus has been less studied and still needs descriptive studies. To analyze morphologically and quantitatively submucosal neurons of the jejunum of 90-day-old healthy rats using different techniques for neuronal staining as a way to provide normality data to compare with future experimental studies. Whole mount preparations of the jejunum were submitted to Giemsa, NADH-diaphorase and NADPH-diaphorase techniques to stain the total neuronal population, more metabolically active subpopulation and subpopulation of nitrergic neurons, respectively. Neurons of the... |
| Tipo: Journal article |
Palavras-chave: Enteric nervous system; Meissner's plexus; Giemsa; NADH-diaphorase; NADPH-diaphorase. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400050 |
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| Lima,Dirlene P.; Azevedo,Jorge F. de; Hermes-Uliana,Catchia; Alves,Gilberto; Sant'ana,Débora M.G.; Araújo,Eduardo J.A.. |
| The objective of this study was to analyze morphometrically the colon wall strata of malnourished rats supplemented with probiotics. Sixteen recently weaned Wistar rats (Rattus norvegicus) were distributed into four groups: animals that received commercial chow (G1, n = 4); animals that received the same feed as G1 and were supplemented with probiotics (G2, n = 4); animals that received chow with 4% of proteins (G3, n = 4); animals that received the same feed as G3 and were supplemented with probiotics (G4, n = 4). After 12 weeks, the proximal colon was collected and submitted to histological processing. Three-µm cuts were stained with H.E., Periodic Acid Schifff (P.A.S.) + diasthasis solution and Alcian Blue (A.B.) pH 2.5 and pH 1.0. The morphometric... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Histology; Large intestine; Malnourishment; Morphometry; Probiotics. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300015 |
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| Sant'Ana,Débora M.G.; Araújo,Eduardo J.A.; Ramos,Diego H.; Hermes-Uliana,Catchia; Natali,Maria Raquel M.. |
| The effects of severe protein malnutrition (4%) on myenteric neurons of Wistar rat duodenum, in relation to a standard 22%-protein diet for rodents, were assessed in this study. Segments of the duodenum from 10 rats from each nutritional group were submitted to the elaboration of whole mounts - 5 stained with Giemsa to determine the total population of myenteric neurons and the others stained by a histochemical method to detect nervous cells through the NADPH-diaphorase enzyme activity for studying the subpopulation of nitrergic neurons. The area of 100 neurons per animal, totalizing 2,000 neurons, were randomly measured by using the Image Pro-Plus®software. Malnourished rats presented 34.38% lower body weight and 10.60% duodenum length reduction when... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteric nervous system; Malnutrition; Myenteric plexus; Nitrergic neurons. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300022 |
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