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Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation BABT
Mustafa,Siti Ratina; Husaini,Ahmad; Hipolito,Cirilo Nolasco; Hussain,Hasnain; Suhaili,Nurshikin; Roslan,Hairul Azman.
Amylase is recognized as one of the important commercial enzymes. This group of enzymes has the ability in hydrolyzing starch into smaller oligosacharides. The present work aimed to determine the optimum fermentation conditions for maximum production of crude amylase enzyme by Aspergillus flavus NSH9 employing response surface methodology (RSM).Central composite design (CCD) was applied to determine the optimal fermentation condition with respect to the four main process parameters such as temperature, initial moisture content, pH and the incubation period. Solid state fermentation (SSF) was performed using 5.0 g of sago hampas inoculated with 1x107sporesmL-1following the experimental design obtained using CCD and further optimized by RSM. The initial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid state fermentation; Aspergillus flavusNSH9; Sago hampas; Central Composite Design; Response Surface Methodology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100416
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Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust BABT
Sing,Ngieng Ngui; Zulkharnain,Azham; Roslan,Hairul Azman; Assim,Zani; Husaini,Ahmad.
Four fungal isolates, SD12, SD14, SD19 and SD20 isolated from the aged sawdust grew on agar plates supplemented with PCP up to a concentration of 100 mg L-1. At high PCP concentration, isolate SD12 showed the highest radial growth rate of 10 mm day-1, followed by SD14 and SD19 both with 4.5 mm day-1 and SD20 with 4.2 mm day-1. Ultrastructural study on the effect of PCP on the PCP tolerant fungi using scanning electron microscope showed that high concentration of PCP caused the collapse of both fungal hyphae and spores. Among the four PCP tolerant fungi examined, isolate SD12 showed the least structural damage at high PCP concentration of 100 mg L-1. This fungal isolate was further characterized and identified as Cunninghamella sp. UMAS SD12. Preliminary...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pentachlorophenol; Fungi; Cunninghamellasp.; Scanning Electron Microscopy; PCP degradation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600811
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