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| Kamikawa, Akihiro; Ichii, Osamu; Sakazaki, Junpei; Ishikawa, Toru. |
| The Cl- secretion via Ca2+-activated Cl- channel (CaCC) is critical for fluid secretion in exocrine glands like the salivary gland. Also in the mammary gland, it has been hypothesized that CaCC plays an important role in the secretion of Cl- and aqueous phase of milk. However, there has been no evidence for the functional expression of CaCC in native mammary secretory (MS) cells of lactating animals. We therefore assessed membrane current in MS cells that were freshly isolated from lactating mice using whole cell patch-clamp techniques. In MS cells, we detected CaCC current that exhibited the following characteristics: 1) Ca2+-dependent activation at the concentrations of sub-micromolar range; 2) voltage-dependent activation; 3) slow kinetics for... |
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Palavras-chave: CaCC; Lactation; Mammary gland; Mammary secretory cell; Patch-clamp. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4553 |
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