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Yuyama,Kamila Tomoko; Pereira,Jadergudson; Maki,Cristina Sayuri; Ishikawa,Noemia Kazue. |
The Amazon has a high diversity of fungi, including species of the genus Daldinia (Ascomycota, Xylariaceae), which produce secondary metabolites with recognized nematicidal and antimicrobial activity. The ecological role of Daldinia is important, as stromata serve as refuges to many insects and arthropodes, and the fungi contribute to the degradation of vegetable organic matter. The aim of this study was to analyze the taxonomic features and mycelial growth conditions in vitro of a Daldinia specimen collected in the Brazilian Amazon. Morphological and molecular studies of the fungus identified it as D. eschscholtzii. To evaluate mycelial growth, we cultivated the fungus at 20, 25, 30, 35, and 40 °C in malt extract-peptone agar (MEPA), malt extract-peptone... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Daldinia eschscholzii; Fungal diversity; Macrofungus; Occurrence. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672013000100001 |
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Faria,Terezinha de Jesus; Ferreira,Rafael Sottero; Yassumoto,Lidiane; Souza,José Roberto Pinto de; Ishikawa,Noemia Kazue; Barbosa,Aneli de Melo. |
An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ocimum gratissimum; Antifungal activity; Eugenol; Phytopathogenic fungi. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700002 |
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Ishikawa,Noemia Kazue; Tahara,Satoshi; Namatame,Tomohiro; Farooq,Afgan; Fukushi,Yukiharu. |
Enokipodins A, B, C, and D are antimicrobial sesquiterpenes isolated from the mycelial culture medium of Flammulina velutipes, an edible mushroom. The presence of a quaternary carbon stereocenter on the cyclopentane ring makes enokipodins A-D attractive synthetic targets. In this study, nine different cytochrome P450 inhibitors were used to trap the biosynthetic intermediates of highly oxygenated cuparene-type sesquiterpenes of F. velutipes. Of these, 1-aminobenzotriazole produced three less-highly oxygenated biosynthetic intermediates of enokipodins A-D; these were identified as (S)-(-)-cuparene-1,4-quinone and epimers at C-3 of 6-hydroxy-6-methyl-3-(1,2,2-trimethylcyclopentyl)-2-cyclohexen-1-one. One of the epimers was found to be a new compound. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial compound cuparene-1; 4-quinone edible mushroom enokitake Flammulina velutipes. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400037 |
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