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Bovine semen thermoresistance tests and their correlation with pregnancy rates after fixed-time artificial insemination Arq. Bras. Med. Vet. Zootec.
Talini,R.; Kozicki,L.E.; Gaievski,F.R.; Polo,G.; Lima,L.G.F.; Santiago,J.; Segui,M.S.; Weiss,R.R.; Galan,T.G.B..
ABSTRACT The objective of the study was to determine whether or not there is a correlation between thermoresistance tests (TT) after semen thawing and pregnancy rate (PR) after fixed-time artificial insemination (FTAI). Four different TT were performed on ten samples used for AI; a rapid test (RTT) (30min / 46°C) and three slow tests (STT): STT1 (60min/38°C), STT2 (180min/38°C), and STT3 (300min/38°C). Two hundred and fifteen multiparous crossbred cows were submitted to FTAI under the following protocol: on day zero (d0) the animals received a P4 device +EB; on d7 PGF2α; on d8 P4 was removed and eCG+EC were administered; IATF was performed on d10. Three gestational diagnoses (G D) were performed on d40, d70 and d120. The mean sperm motility (%) in RTT and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sperm motility; Casa; Pregnancy rate; Beef cows; Semen thermoresistance test.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000602085
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Viability of ovine spermatozoa collected from epididymides stored at 18°-25°C for 48 hours post mortem Arq. Bras. Med. Vet. Zootec.
Bergstein-Galan,T.G.; Weiss,R.R.; Barbosa,T.S.R.; Kozicki,L.E.; Bicudo,S.D..
ABSTRACT The objectives of this study were to verify the time during which viable ovine spermatozoa could be recovered from the cauda epididymis kept at ambient temperature (18-25°C). Sperm collected in an artificial vagina (AV) were used as control. Spermatozoa samples were collected with an AV and from epididymis at 0 (G0), 6 (G6), 12 (G12), 24 (G24), and 48 (G48) hours post mortem. Total motility (TM), progressive motility (PM), hypo-osmotic membrane integrity test (HOST) and morphological changes were assessed. TM decreased (P<0.05) from 24 hours post mortem (70.0±1.9%) compared to AV (86.4±1.0%). PM decreased (P<0.05) from 12 hours after death (31.3±4.0%) compared to AV group (73.2±1.4%). The percentage of viable cells in HOST decreased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ram; Epididymis; Room temperature.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401023
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