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Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis Rev. Ciênc. Agron.
Victório,Cristiane Pimentel; Lage,Celso Luiz Salgueiro; Sato,Alice.
The high demand for plant material from Calendula officinalis in the production of herbal medicines and cosmetics, turns the technique of plant-tissue culture into one of the alternatives for the improvement of crops over a short period of time. A protocol for tissue culture was developed from segments of seedlings of C. officinalis, in order to improve the proliferation of shoots and roots. We used a Murashige and Skoog (MS½N) medium, reduced to half the concentrations of NH4NO3 and KNO3 to verify the effect of different types of explants (basal, intermediate, and apical), a medium containing beach sand as support instead of agar, and the effect of auxins and cytokinins (TDZ tidiazuron; BAP, 6-benzylaminopurine, IAA, indol-3-acetic acid, IBA,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Shoots (Plants); Plant-tissue culture; Plant-growth regulators.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000300017
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Standardized production of Phyllanthus tenellus Roxb. by plant tissue culture Rev. Ciênc. Agron.
Victório,Cristiane Pimentel; Henriques,Anaize Borges; Tavares,Eliana Schwartz; Esquibel,Maria Apparecida; Lage,Celso Luiz Salgueiro.
Exigencies as ethic plant raw material are part of the needs of modern phytotherapy. Micropropagation offers opportunities to obtain mass propagation of superior genotypes in short time. This study aimed to develop a protocol of direct and indirect organogenesis of Phyllanthus tenellus Roxb. Nodal segments from plantlets obtained by in vitro germination were subcultured in modified Murashige and Skoog medium added with different plant growth regulators: IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), GA3 (3-giberelic acid) and KIN (kinetin). The highest proliferation rate was obtained using the combinations: IBA, KIN + GA3 (3.5 mg L-1) and IBA + KIN (2.4 mg L-1). Rooting was intensified after 40 days, reaching 100% for all media with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Callus; Micropropagation; Medicinal plant.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902010000200015
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Changes in electrophoretic profiles of Ipomoea batatas (sweet potato) induced by gamma radiation BABT
Lage,Celso Luiz Salgueiro; Vasconcellos,Alexandre Guimarães; Silva,Nina Claudia Barboza da; Esquibel,Maria Apparecida.
The ability of nodal segments of Ipomoea batatas to differentiate shoots and roots was evaluated after gamma irradiation. Shoot differentiation was less sensitive to irradiation than roots. However, at 90 Gy, no shoot was able to regenerate a new plant; in contrast 76 % of the roots from irradiated nodal segments continued to grow. The gamma radiation also induced changes in electrophoretic profiles of peroxidases of storage roots. Plants originated from irradiated storage roots presented changes in leaf peroxidase profiles very similar to those produced by leaves directly irradiated. The peroxidase profile of absorbent roots from irradiated storage roots was different from that obtained from directly irradiated absorbent roots.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidases; Gamma radiation; Sweet potato; Ipomoea batatas; Convovulaceae.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200008
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Vasodilator activity of extracts of field Alpinia purpurata (Vieill) K: Schum and A. zerumbet (Pers.) Burtt et Smith cultured in vitro BJPS
Victório,Cristiane Pimentel; Kuster,Ricardo Machado; Moura,Roberto Soares de; Lage,Celso Luiz Salgueiro.
Nowadays, the high blood pressure is one of the main causes of death and cardiovascular diseases. Vasodilator drugs are frequently used to treat arterial hypertension. Experiments were undertaken to determine whether hydroalcoholic extracts obtained from leaves of field-grown Alpinia purpurata and A. zerumbet cultured in vitro under different plant growth regulators induce a vasodilator effect on Wistar rat mesenteric vascular bed pre-contracted with norepinephrine. Plant extracts were able to induce a long-lasting endothelium-dependent vasodilation. Efficiency on activity of A. purpurata reached 87% at concentration of 60 μg. The extract of A. zerumbet maintained in medium containing IAA, induced the relaxation (17.4%) at 90 μg, as compared to the control...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpinia purpurata (Vieill) K. Schum./pharmacognosy; A. zerumbet (Pers.) Burtt et Smith./pharmacognosy; Natural vasodilators/experimental studies; Plant extracts/vasodilator action; Plant tissue/culture; Endothelium-dependent; Flavonoids; Medicinal Plant; Plant growth/regulators; Zingiberaceae.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300017
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