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| Laha, R; Sasmal, N.K; Bandyopadhyay, S. |
| Trypanosoma evansi from buffalo, cattle and horse isolates were purified by Diethylaminoethyl cellulose column chromatography. The purified trypanosomes from each sources were used separately for preparation of whole cell lysate antigens. A comparative study on polypeptide pattern of whole cell lysate antigens of these three isolates of T. evansi was studied. It showed a total of 11 dominant polypeptide bands with relative molecular weight ranging from 95 to 13 kDa, when resolved in 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. Except some minor differences between the relative molecular weight of isolates in a particular region, the polypeptide profiles of these three isolates did not resolve any... |
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Palavras-chave: Buffalo; Cattle; Horse; Polypeptide profiles; Trypanosoma evansi. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1833 |
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| Laha, R; Das, M; Goswami, A; Singh, P. |
| High rise of temperature (41.3℃), hemoglobinurea, anorexia, decrease milk production, anemia and diarrhea were observed in a cross-bred cow of aged 4 years, maintained in the dairy farm of ICAR research complex for North Eastern Hill Region, Umiam, Meghalaya, India, in its 3 months of lactation. After examination of Giemsa stained blood smears and molecular diagnosis using polymerase chain reaction, the disease was diagnosed as babesiosis caused by Babesia bigemina. The animal was treated successfully with a single injection of 4,4' diamidine diazoamine benzene diaceturate in recommended dose. No parasite was detected by examination of Giemsa stained blood smears after a period of 48 hours post treatment onwards. A total loss of 51.6 liter milk production... |
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Palavras-chave: Babesia bigemina; Cow; Loss; Milk production. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3539 |
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