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| Richini-Pereira,Virginia Bodelão; Marson,Pamela Merlo; Hayasaka,Enio Yoshinori; Victoria,Cassiano; Silva,Rodrigo Costa da; Langoni,Hélio. |
| Background Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens asLeishmaniaspp., offering new opportunities for epidemiological studies of this infection.Methods This study aimed to evaluate the presence ofLeishmaniaspp. andLeishmania chagasiDNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals.Results DNA was detected in tissues of oneCavia aperea(Brazilian guinea pig), fiveCerdocyon thous(crab-eating fox), oneDasypus septemcinctus(seven-banded armadillo), twoDidelphis albiventris(white-eared opossum), oneHydrochoerus hydrochoeris(capybara), twoMyrmecophaga tridactyla(giant anteater), oneProcyon cancrivorus(crab-eating... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Road-killed animal; Leishmaniaspp; Leishmania chagasi; PCR; Zoonosis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200331 |
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| Braga,Audrey Rennó Campos; Langoni,Hélio; Lucheis,Simone Baldini. |
| Background: This study aimed to evaluate the occurrence of Leishmaniaspp. in dogs and cats from Botucatu, São Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area.Results: Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Cat; Diagnosis; Leishmaniasis; Zoonosis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200323 |
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| Shimabukuro,Fábio Hiroto; Domingues,Paulo Francisco; Langoni,Hélio; Silva,Aristeu Vieira da; Pinheiro,Júlia Plombon; Padovani,Carlos Roberto. |
| Com o intuito de se estudar a presença de suínos portadores renais de leptospiras foram colhidas 131 amostras sanguíneas e os respectivos rins de animais durante o abate em abatedouro da região de Botucatu-SP. Pela prova de Soroaglutinação Microscópica obteve-se 48 amostras sorológicas positivas para um ou mais sorovar de Leptospira spp., com uma taxa de ocorrência de anticorpos anti-leptospira de 36,64%, e maior importância para o sorovar icterohaemorrhagiae. Para a pesquisa do agente nos rins, das 88 amostras renais submetidas a cultura em meio de EMJH e analisadas pela prova de PCR, foi isolado e detectado o agente em uma única amostra renal, pertencente a um animal soropositivo. Embora não tenha sido possível a comparação estatística, em termos de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirose animal; Suínos; Reação em cadeia pela polimerase em animal; Isolamento animal. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000400002 |
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| Cunha,Eva Laurice Pereira; Silva,Rodrigo Costa da; Da Silva,Aristeu Vieira; Langoni,Hélio. |
| The present work aimed to verify the effects of Baypamun®HK on the survival of albinic mice, experimentally infected with Toxoplasma gondii RH strain, treated or not with sulfadiazine-pirimetamine, as well as on the formation and the number of brain cysts. Four groups of 20 mices were inoculated with 10(5) tachizoites, subcutaneously, and undergone to different treatments. Serum tests were carried out by indirect immunofluorescence and samples of brain, liver and lung were collected from animals who died for cytological and brain cysts examinations. After 60 days, all survivors were sacrificed. Isolated Baypamun®HK didn't present a protective action against the infection by T. gondii, but its association sulfadiazine-pirimetamine, a specific treatment... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Baypamun®HK; Indirect immunofluorescence; Mice. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000300004 |
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| Oliveira,Pollyanne Raysa Fernandes de; Soares,Larice Bruna Ferreira; Borges,Jonas de Melo; Barrosa,Noelle de Castro; Langoni,Hélio; Brandespim,Daniel Friguglietti; Pinheiro Junior,José Wilton; Mota,Rinaldo Aparecido. |
| ABSTRACT The objective of this study was to evaluate the occurrence of anti-Leptospira spp. antibodies in female buffalo in the state of Pernambuco. A total of 123 female buffalo blood samples were collected from five properties distributed in the state of Pernambuco. The microscopic agglutination test was used to study anti-Leptospira spp. antibodies. The occurrence of anti-Leptospira spp. antibodies was 28.5% (35/123; CI 20.7–37.3%) and on different properties, the occurrence ranged from 28.6% to 80.0%, with 100% of the properties showing animals with positive results. The serovars of the serogroup Sejroe with a higher incidence were Hardjoprajtino (CTG strain, 49.1%) and Hardjo (Prajtino genotype, 43.2%), followed by serogroup Grippotyphosa with the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Epidemiology; Bubalusbubalis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400795 |
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| Monteiro,Thamillys Rayssa Marques; Rocha,Katarine de Souza; Mesquita,Gleiciane Schupp de Sena; Castro,Danniel Carvalho de; Miranda,Stefânia Araújo; Paz,Giselle Souza da; Langoni,Hélio; Moraes,Carla Cristina Guimarães de. |
| ABSTRACT: Although, serological studies for leptospirosis in iguanas have already been performed, there is no report in the Amazon region. Therefore, the present study aimed to investigate the presence of anti-Leptospira sp. in free living Iguanas-verdes (Iguana iguana) of Mangal das Garças Park, metropolitan region of Belém, Pará, Northern region of Brazil. Twenty-nine blood samples of Iguana-verde (I. iguana) were collected from Mangal das Garças Park. For the serological diagnosis of Leptospira sp. it was employed microscopic agglutination test (MAT) with 25 live antigens of Leptospira sp. as antigens. It was observed that all samples were non-reactive, suggesting no exposure to the agent. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reptiles; Leptospirosis; Wild animals; Amazon. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900452 |
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| Langoni,Hélio; Del Fava,Claudia; Cabral,Kenio de Gouvêa; Silva,Aristeu Vieira da; Chagas,Sérgio Aguiar Pacheco. |
| Foram estudadas aglutininas antileptospíricas em 403 amostras de soro de búfalos, provenientes de sete Municípios do Vale do Ribeira, Estado de São Paulo, coletadas no período de janeiro de 1992 a junho de 1993. Utilizou-se o teste de soroaglutinação microscópica, considerando-se positivas as amostras cujo título fosse igual ou superior a 100. O maior título encontrado foi 1600 para o sorovar bratislava (1 amostra), seguido de 800 para wolffi (4 amostras). Do total, 152 (37,7%) das amostras foram positivas, sendo que, dentre os sorovares testados, a prevalência em ordem decrescente foi: wolffi (68, 44,8%), icterohaemorrhagiae (51, 33,6%), hardjo (51, 33,6%), castellonis (25, 16,5%), djasiman (12, 7,9%), grippotyphosa (10, 6,6%), pomona (8, 5,2%),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Soroaglutinação microscópica; Búfalos. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000200019 |
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