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| Girault, Mathias; Siano, Raffaele; Labry, Claire; Latimier, Marie; Jauzein, Cecile; Beneyton, Thomas; Buisson, Lionel; Del Amo, Yolanda; Baret, Jean-christophe. |
| Adaptation of cell populations to environmental changes is mediated by phenotypic variability at the single-cell level. Enzyme activity is a key factor in cell phenotype and the expression of the alkaline phosphatase activity (APA) is a fundamental phytoplankton strategy for maintaining growth under phosphate-limited conditions. Our aim was to compare the APA among cells and species revived from sediments of the Bay of Brest (Brittany, France), corresponding to a pre-eutrophication period (1940’s) and a beginning of a post-eutrophication period (1990’s) during which phosphate concentrations have undergone substantial variations. Both toxic marine dinoflagellate Alexandrium minutum and the non-toxic dinoflagellate Scrippsiella acuminata were revived from... |
| Tipo: Text |
Palavras-chave: Climate-change ecology; Microbial ecology. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00679/79097/81568.pdf |
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| Delebecq, Gaspard; Schmidt, Sabine; Ehrhold, Axel; Latimier, Marie; Siano, Raffaele. |
| The biological processes involved in the preservation, viability, and revival of long‐term dormant dinoflagellate cysts buried in sediments remain unknown. Based on studies of plant seed physiology, we tested whether the revival of ancient cysts preserved in century‐old sediments from the Bay of Brest (France) could be stimulated by melatonin and gibberellic acid, two molecules commonly used in seed priming. Dinoflagellates were revived from sediments dated to approximately 150 years ago (156 ± 27, 32 cm depth), extending the known record age of cyst viability previously established as around one century. A culture suspension of sediments mixed with melatonin and gibberellic acid solutions as biostimulants exhibited germination of 11 dinoflagellate taxa... |
| Tipo: Text |
Palavras-chave: Alexandrium; Cyst; Dinoflagellate; Gibberellic acid; Melatonin; Paleoecology; Priming; Scrippsiella; Resurrection ecology. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00627/73960/73321.pdf |
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| Mondeguer, Florence; Souard, Florence; Guitton, Yann; Latimier, Marie; Quere, Julien; Sibat, Manoella; Hess, Philipp; Siano, Raffaele. |
| Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a untargeted metabolomics approach by LC-HRMS to study the possible... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00440/55199/56664.pdf |
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| Lema, Anais Kimberley; Latimier, Marie; Nezan, Elisabeth; Fauchot, Juliette; Le Gac, Mickael. |
| The factors responsible for inducing the synthesis of toxins and responses from toxic phytoplankton blooms remain unclear. In this study we compare the influence of genotypic (at both the intra and interspecific levels) and environmental factors (nutrient concentration and ratio) on growth (in terms of cell densities) and domoic acid (DA) production in three Pseudo-nitzschia species: P. australis, P.pungens and P.fradulenta. A strong phosphate effect was detected. More precisely, a low initial concentration in phosphate, even at high initial nitrogen and silicate concentrations, induced the highest DA concentrations and the lowest cell densities in all strains/species studied. In contrast, a low initial concentration of nitrogen and silicate combined, with... |
| Tipo: Text |
Palavras-chave: Pseudo-nitzschia strains; Domoic acid; Ratio; Phosphate; Nitrogen. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00377/48849/49397.pdf |
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| Mondeguer, Florence; Souard, Florence; Guitton, Yann; Allard, Pierre-marie; Elie, Nicolas; Sibat, Manoella; Latimier, Marie; Quere, Julien; Wolfender, Jean-luc; Siano, Raffaele; Hess, Philipp. |
| Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a metabolomic approach to study the possible adaptive responses of... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00464/57591/59769.pdf |
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| Lema, Anais Kimberley; Metegnier, Gabriel; Quéré, Julien; Latimier, Marie; Youenou, Agnes; Lambert, Christophe; Fauchot, Juliette; Le Gac, Mickael. |
| Untangling the functional basis of divergence between closely related species is a step towards understanding species dynamics within communities at both the evolutionary and ecological scales. We investigated cellular (i.e: growth, domoic acid production, nutrient consumption) and molecular (transcriptomic analyses) responses to varying nutrient concentrations across several strains belonging to three species of the toxic diatom genus Pseudo-nitzschia. Three main results were obtained. First, strains from the same species displayed similar transcriptomic, but not necessarily cellular, responses to the experimental conditions. It showed the importance of considering intra-specific diversity to investigate functional divergence between species. Second, a... |
| Tipo: Text |
Palavras-chave: Phenotype; Transcriptomics; Pseudo-nitzschia; Divergence; Nutrients. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00482/59392/62157.pdf |
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