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| Registros recuperados: 20 | |
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| Certain, Cassandre; Della Patrona, Luc; Gunkel-grillon, Peggy; Leopold, Audrey; Soudant, Philippe; Le Grand, Fabienne. |
| Cultivation of salt-tolerant crops help to face to irreversible global salinization of freshwater and soils. In New-Caledonia, three halophytes are candidates for saline crops, Salsola australis R.Br., Suaeda maritima (L.) Dumort and Enchylaena tomentosa R.Br. Their success and quality depend yet on availabilities of salinity and essential nutrients in agrosystems. So, we investigated effects of three salinities, i.e., control moderate and high, and five nitrogen ratios, i.e., 100:0, 75:25, 50:50, 25:75 or 0:100 NO3−-N:NH4+-N ratio on their growth and functional value for fatty acids and antioxidants. Results show that the leaf fatty acid and antioxidant profiles of species, emphasize their good potential to become functional crop products, based on... |
| Tipo: Text |
Palavras-chave: Halophyte; Salsola australis; Suaeda maritima; Enchylaena tomentosa; Salt tolerance; Nitrogen form; Fatty acid; Phenolic compounds; Antioxidant activity. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00682/79411/81960.pdf |
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| Mathieu-resuge, Margaux; Schaal, Gauthier; Kraffe, Edouard; Corvaisier, Rudolph; Lebeau, Oanez; Lluch-cota, Salvador E.; Salgado García, Rosa L.; Kainz, Martin J.; Le Grand, Fabienne. |
| The trophic fate of various food sources is of central interest for ecologists, yet not well understood in coastal lagoon food webs. In this field study, fatty acids (FA), stable isotopes (SI), and compound-specific isotopic analysis (CSIA) on FA were used to investigate how diets from oceanic and local sources are retained in a bivalve species (Spondylus crassisquama; Lamarck 1819) along a transect in the Ojo de Liebre lagoon (BCS, Mexico). Results from SI and FA indicated the contribution of oceanic diatoms at the entrance of the lagoon, through 15N enrichment, and higher proportions of 16:1n-7 and 20:5n-3 in digestive glands. In the inner bay, higher abundance of 18-carbon FA (18:1n-9, 18:3n-3, 18:4n-3) suggested a higher contribution of... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00501/61306/64914.pdf |
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| Mathieu-resuge, Margaux; Le Grand, Fabienne; Schaal, Gauthier; Lluch-cota, Salvador E; Racotta, Ilie S; Kraffe, Edouard; Todgham, Anne. |
| Bivalves’ physiological functions (i.e. growth, reproduction) are influenced by environmental variability that can be concomitant with trophic resource variations in terms of quality and quantity. Among the essential molecules that bivalves need to acquire from their diet to maintain physiological functions, fatty acids (FAs) such as polyunsaturated fatty acids (e.g. 20:4n-6 (arachidonic acid), 20:5n-3 (eicosapentaenoic acid) and 22:6n-3 (docosahexaenoic acid)) have been described to play a critical role. The present study examined the FA composition of gill membrane lipids of two bivalve species, Nodipecten subnodosus and Spondylus crassisquama, sampled in a coastal lagoon of the Northeastern Pacific (Ojo de Liebre, Mexico), at two contrasting locations... |
| Tipo: Text |
Palavras-chave: Bivalves; Coastal lagoon; Gill membrane fatty acids; Diet influence; Physiological regulation; Trophic ecophysiology. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00648/75991/76893.pdf |
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| Roussel, Sabine; Caralp, Claire; Leblanc, Catherine; Le Grand, Fabienne; Stiger-pouvreau, Valerie; Coulombet, Céline; Le Goïc, Nelly; Huchette, Sylvain. |
| The commercial culture of Haliotis tuberculata has recently started in Europe. As abalone is herbivorous, the use of local collected algae as feed may appear advantageous. The nutritional value of eight monospecific seaweed diets was studied using Palmaria palmata (Rhodophyta), filamentous algae, mainly Gracilaria sp. (Rhodophyta), Enteromorpha sp. and Ulva lactuca (Chlorophyta), together with Saccharina latissima, Saccorhiza polyschides, Laminaria digitata and Laminaria hyperborea (Ochrophyta, Phaeophyceae) and a mixed macroalgal diet. An integrative approach consisted in monitoring the seasonal composition changes of these algae in terms of protein, lipid, soluble carbohydrate, fatty acid and amino-acid contents, and to relate it to seasonal growth and... |
| Tipo: Text |
Palavras-chave: Abalone; Algae; Reproduction; Growth; Protein. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00509/62066/66246.pdf |
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| Lavaud, Romain; Artigaud, Sebastien; Le Grand, Fabienne; Donval, Anne; Soudant, Philippe; Flye-sainte-marie, Jonathan; Strohmeier, Tore; Strand, Oivind; Leynaert, Aude; Beker, Beatriz; Chatterjee, Arnab; Jean, Fred. |
| We combined the use of pigments, fatty acids and sterols as biomarkers of the seasonal variation in food sources of the great scallop Pecten maximus. From March to October 2011, on a biweekly to twice-weekly basis, scallops and seawater from the water column and the water-sediment interface were collected in the Bay of Brest (Brittany, France). Pigment compositions in the seawater and in the stomach and rectum content of the scallops were analyzed by HPLC. Fatty acids and sterols from digestive gland (DG) tissue were analyzed by gas chromatography. Potential relationships between the temporal proportion of each marker in the environment and in the digestive tract were tested using multivariate analysis. Proportions of diatoms and Dinophyceae biomarkers... |
| Tipo: Text |
Palavras-chave: Trophic ecology; Food sources; Pigments; Fatty acids; Trophic marker; Phytoplankton; Pecten maximus. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00431/54244/55772.pdf |
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| Remize, Marine; Planchon, Frederic; Loh, Ai Ning; Le Grand, Fabienne; Lambert, Christophe; Bideau, Antoine; Bidault, Adeline; Corvaisier, Rudolph; Volety, Aswani; Soudant, Philippe. |
| The synthetic pathways responsible for the production of the polyunsaturated fatty acids 22:6n-3 and 20:5n-3 were studied in the Dinophyte Alexandrium minutum. The purpose of this work was to follow the progressive incorporation of an isotopic label (13CO2) into 11 fatty acids to better understand the fatty acid synthesis pathways in A. minutum. The Dinophyte growth was monitored for 54 h using high-frequency sampling. A. minutum presented a growth in two phases. A lag phase was observed during the first 30 h of development and had been associated with the probable temporary encystment of Dinophyte cells. An exponential growth phase was then observed after t30. A. minutum rapidly incorporated 13C into 22:6n-3, which ended up being the most 13C-enriched... |
| Tipo: Text |
Palavras-chave: PUFA synthesis; 22:6n-3; DHA; PKS pathway; Dinophyte; Alexandrium minutum; Encystment. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00654/76562/77688.pdf |
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| Long, Marc; Tallec, Kevin; Soudant, Philippe; Lambert, Christophe; Le Grand, Fabienne; Sarthou, Geraldine; Jolley, Dianne; Hegaret, Helene. |
| Harmful microalgal blooms are a threat to aquatic organisms, ecosystems and human health. Toxic dinoflagellates of the genus Alexandrium are known to produce paralytic shellfish toxins and to release bioactive extracellular compounds (BECs) with potent cytotoxic, hemolytic, ichtyotoxic and allelopathic activity. Negative allelochemical interactions refer to the chemicals that are released by the genus Alexandrium and that induce adverse effects on the physiology of co-occurring protists and predators. Releasing BECs gives the donor a competitive advantage that may help to form dense toxic blooms of phytoplankton. However BECs released by Alexandrium minutum are uncharacterized and it is impossible to quantify them using classical chemical methods.... |
| Tipo: Text |
Palavras-chave: Harmful algal bloom; Alexandrium; Allelopathy; Cytotoxicity; PAM fluorometry; Bioassay. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00453/56424/58116.pdf |
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| Da Costa, Fiz; Le Grand, Fabienne; Quere, Claudie; Bougaran, Gael; Cadoret, Jean-paul; Robert, Rene; Soudant, Philippe. |
| Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete and N-reduced conditions to determine the effects of N supply in culture medium and growth phase on microalgal physiology and biochemistry. These two T. lutea strains were compared in terms of growth, morphology, associated free-living bacterial community, viability, intracellular lipid content (as measured by Bodipy staining), chlorophyll autofluorescence, and biochemical composition, with a focus on lipid class and fatty acid compositions. The standard strain (T) reached higher cell counts regardless of N supply in culture medium. In both T. lutea strains, microalgal final cell density was significantly lower in N-reduced medium. Carbohydrates... |
| Tipo: Text |
Palavras-chave: Tisochrysis lutea; Nitrogen limitation; Growth phase; Flow cytometry; Fatty acids; Lipid classes. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00399/51074/51936.pdf |
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| Couturier, Lydie; Michel, Loic; Amaro, Teresa; Budge, Suzanne M; Da Costa, Elisabete; De Troch, Marleen; Di Dato, Valeria; Fink, Patrick; Giraldo, Carolina; Le Grand, Fabienne; Loaiza, Iván; Mathieu-resuge, Margaux; Nichols, Peter D; Parrish, Christopher C; Sardenne, Fany; Vagner, Marie; Pernet, Fabrice; Soudant, Philippe; Browman, Howard. |
| Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA... |
| Tipo: Text |
Palavras-chave: Aquatic nutrition; Biomarkers; Fatty acids; Marine and freshwater lipids; Methods; Recommended practices. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00651/76279/77252.pdf |
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| Remize, Marine; Planchon, Frederic; Loh, Ai Ning; Le Grand, Fabienne; Bideau, Antoine; Le Goic, Nelly; Fleury, Elodie; Miner, Philippe; Corvaisier, Rudolph; Volety, Aswani; Soudant, Philippe. |
| The present study sought to characterize the synthesis pathways producing the essential polyunsaturated fatty acid (PUFA) 20:5n-3 (EPA). For this, the incorporation of 13C was experimentally monitored into 10 fatty acids (FA) during the growth of the diatom Chaetoceros muelleri for 24 h. Chaetoceros muelleri preferentially and quickly incorporated 13C into C18 PUFAs such as 18:2n-6 and 18:3n-6 as well as 16:0 and 16:1n-7, which were thus highly 13C-enriched. During the experiment, 20:5n-3 and 16:3n-4 were among the least-enriched fatty acids. The calculation of the enrichment percentage ratio of a fatty acid B over its suspected precursor A allowed us to suggest that the diatom produced 20:5n-3 (EPA) by a combination between the n-3 (via 18:4n-3) and n-6... |
| Tipo: Text |
Palavras-chave: Synthesis pathway; Diatom; 20:5n-3 (EPA); Chaetoceros muelleri; Acyl-editing mechanism; Compound-specific isotope analysis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00630/74257/73887.pdf |
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| Sardenne, Fany; Bodin, Nathalie; Metral, Luisa; Crottier, Anais; Le Grand, Fabienne; Bideau, Antoine; Brisset, Blandine; Bourjea, Jerome; Saraux, Claire; Bonhommeau, Sylvain; Kerzerho, Vincent; Bernard, Serge; Rouyer, Tristan. |
| Various protocols are currently used to study marine lipids, but there is a growing interest in working on dry samples that are easier to transport. However, reference protocols are still lacking for dry samples. In order to make recommendations on this use, lipid classes and fatty acids (FA) obtained from six analytical protocols using two different tissue states (dry vs wet) and three extraction methods (automat vs manual potter vs leaving the solvent to work on tissue) were compared. Three dry storage modes of tissue (freezer vs gas nitrogen vs dry room) during one and three months were also compared. These comparisons were made on seven marine species with different lipid profiles, including fishes, crustaceans and mollusks. Lipid classes and FA... |
| Tipo: Text |
Palavras-chave: Degradation; Freeze-drying; Lyophilization; Lipid class; Marine animal; Protocol. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00467/57898/60189.pdf |
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| Garnier, Matthieu; Bougaran, Gael; Pavlovic, Marija; Berard, Jean-baptiste; Carrier, Gregory; Charrier, Aurelie; Le Grand, Fabienne; Lukomska, Ewa; Rouxel, Catherine; Schreiber, Nathalie; Cadoret, Jean-paul; Rogniaux, Helene; Saint-jean, Bruno. |
| Haptophytes are a diverse monophyletic group with a worldwide distribution, known to be significantly involved in global climate regulation in their role as a carbon sink. Because nitrogen is a major limiting macronutrient for phytoplankton in oceans and for cultures of microalgae, understanding the involvement of nitrogen availability in haptophyte carbon partitioning is of global and biotechnological importance. Here, we made an ecophysiological study coupled with comprehensive large scale proteomic analysis to examine differences of behavior in reaction to nitrogen availability changes between a wild type strain of Tisochrysis lutea (WTc1) and a mutant strain (2Xc1) known to accumulate more storage lipids. Strains were grown in chemostats and studied... |
| Tipo: Text |
Palavras-chave: Algae; Isochrysis; Lipids; Nitrogen; Reverse genomic: proteomic. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00356/46702/46904.pdf |
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| Mathieu-resuge, Margaux; Le Grand, Fabienne; Schaal, Gauthier; Kraffe, Edouard; Lorrain, Anne; Letourneur, Yves; Lemonnier, Hugues; Benoît, Julie; Hochard, Sébastien. |
| Deposit-feeding sea cucumbers are efficient nutrient recyclers and have the potential to contribute to the limitation of organic matter load in polyculture or integrated aquaculture systems. Assessing how they assimilate organic matter originating from other farmed species is therefore important for the development of such multi-species farming systems. Here, a coupled stable isotope − fatty acid approach was used to characterize the assimilation of organic matter from shrimp (Penaeus stylirostris) farming by Holothuria scabra in an experimental culture system. H. scabra were reared in mesocosms on shrimp farming-originating sediment with and without additional food sources (maize and fish meals). Although fatty acid results did indicate that... |
| Tipo: Text |
Palavras-chave: Fatty acids; Stable isotopes; Rotational co-culture; Shrimp-farming; Holothurid. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00624/73589/73023.pdf |
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| Le Croizier, Gael; Schaal, Gauthier; Gallon, Regis; Fall, Massal; Le Grand, Fabienne; Munaron, Jean-marie; Rouget, Marie-laure; Machu, Eric; Le Loc'H, Francois; Lae, Raymond; De Morais, Luis Tito. |
| The link between trophic ecology and metal accumulation in marine fish species was investigated through a multi-tracers approach combining fatty acid (FA) and stable isotope (SI) analyses on fish from two contrasted sites on the coast of Senegal, one subjected to anthropogenic metal effluents and another one less impacted. The concentrations of thirteen trace metal elements (As, Cd, Co, Cr, Cu, Fe, Li, Mn, Ni, Pb, Sn, U, and Zn) were measured in fish liver. Individuals from each site were classified into three distinct groups according to their liver FA and muscle SI compositions. Trace element concentrations were tested between groups revealing that bioaccumulation of several metals was clearly dependent on the trophic guild of fish. Furthermore,... |
| Tipo: Text |
Palavras-chave: Trace elements; Biochemical tracers; Diet; Contamination; Senegal; Tropical fish. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00348/45908/45707.pdf |
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| Sardenne, Fany; Diaha, N'Guessan Constance; Amande, Monin Justin; Zudaire, Iker; Couturier, Lydie; Metral, Luisa; Le Grand, Fabienne; Bodin, Nathalie. |
| In the Gulf of Guinea, bigeye Thunnus obesus (BET) and yellowfin tuna Thunnus albacares (YFT) are important for commercial fisheries and play a prominent ecological role as top predators. Using fatty acid profiles and carbon and nitrogen stable isotopes we examined their niche partitioning in this understudied region. Niche overlap was high (>70%), similar to percentages in other ocean basins. BET occupied a higher trophic position than YFT and fed on deeper prey (high δ15N values and high proportions of mono-unsaturated fatty acids). The trophic position of YFT decreased slightly in the last 15 years (δ15N values decrease ~0.5 ‰) suggesting a change in epipelagic communities, as observed in the eastern Pacific Ocean. Ontogenic changes were limited to... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00436/54796/56254.pdf |
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| Mathieu-resuge, Margaux; Kraffe, Edouard; Le Grand, Fabienne; Boens, Andy; Bideau, Antoine; Lluch-cota, Salvador E.; Racotta, Ilie S.; Schaal, Gauthier. |
| Understanding the nature and origins of food sources supporting coastal lagoon-inhabiting organisms is necessary to evaluate the ecological status of such ecosystems. The trophic ecology of a bivalve specie Spondylus crassisquama was studied in the Ojo de Liebre lagoon (Baja California, Mexico), combining stable isotope (SI), fatty acid (FA) and sterols analyses along a transect under oceanic influences. The second objective of the study was to investigate if sterols compositions give complementary information to those obtained from FA and SI. Temporal and spatial patterns of the three biomarkers in bivalve tissues suggest oceanic inputs at the mouth of the lagoon, while the inner station was characterized by a contribution of local sources including an... |
| Tipo: Text |
Palavras-chave: Coastal zone; Ojo de liebre; Suspension-feeders; Multi-tracer analyses; Fatty acids; Stables isotopes; Sterols. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00484/59521/62494.pdf |
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| Borcier, Elodie; Charrier, Gregory; Couteau, Jérôme; Maillet, Géraldine; Le Grand, Fabienne; Bideau, Antoine; Waeles, Mathieu; Le Floch, Stephane; Amara, Rachid; Pichereau, Vianney; Laroche, Jean. |
| The objective of this study was to develop an integrative approach in ecotoxicology (from biomarkers to population genetics) to assess the ecological status of fish populations. Flounders (Platichthys flesus) collected after the spawning season in the heavily polluted Seine estuary were compared with the moderately polluted Bay of Douarnenez. The muscle energetic reserves were highly depleted in Seine vs. Douarnenez fish. The Seine fish displaying a reduced capacity to manage the oxidative stress and a higher energetic metabolism. An increase in the content of muscle membrane phospholipids (sphingomyelin, phosphatidylserine, free sterols) was detected in the Seine vs. Douarnenez fish. The data integration allowed to hypothesize relationships between... |
| Tipo: Text |
Palavras-chave: Platichthys flesus; Estuaries; Biomarkers; Bioenergetics; Polar lipids; Population genetics. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00658/76981/78238.pdf |
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| Roussel, Sabine; Poitevin, Pierre; Day, Robert; Le Grand, Fabienne; Stiger‐pouvreau, Valérie; Leblanc, Catherine; Huchette, Sylvain. |
| While population foraging behaviour of herbivores has been extensively studied, individual choice is still poorly understood. Very few studies have focused on the individual consistency of foraging behaviour in marine herbivores. Because marine ectotherms are strongly influenced by their environment and because a mixed diet is appropriate for herbivores, we hypothesized that Haliotis tuberculata, a large marine gastropod, would not exhibit significant individual consistency in foraging activity and would display generalist food choices. To test these hypotheses, the behaviour of 120 abalone was studied using a choice test of eight macroalgal species over 3 weeks, with video recording 24 hr a day. In addition, primary components, secondary metabolites and... |
| Tipo: Text |
Palavras-chave: Food choice; Foraging activity; Individual consistency; Intraclass correlation; Marine herbivore; Mollusc; Proportion similarity index. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00613/72465/71613.pdf |
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| Long, Marc; Tallec, Kevin; Soudant, Philippe; Le Grand, Fabienne; Donval, Anne; Lambert, Christophe; Sarthou, Geraldine; Jolley, Dianne F.; Hegaret, Helene. |
| Allelochemical interactions are likely to be a contributing factor explaining the success of large blooms of the harmful marine dinoflagellate Alexandrium, however, the physiological mechanisms of allelochemical interactions remain poorly described. Here we investigated the sub-lethal effects (on an hourly scale) of a filtrate containing allelochemicals from Alexandrium minutum on the physiology of the common diatom Chaetoceros muelleri. The filtrate induced deleterious effects to the diatom physiology within only 30 min of exposure. Esterase activity and photosynthesis were drastically inhibited, with up to 34% of the population being metabolically inactive and up to 30% reduction in photosystem II quantum yield when exposed to the filtrate. In addition,... |
| Tipo: Text |
Palavras-chave: Allelopathy; Alexandrium; Metabolism; Photoinhibition; Lipids; Pigments. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00460/57133/59748.pdf |
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| Registros recuperados: 20 | |
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