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| Registros recuperados: 65 | |
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| Gueguen, Yannick; Montagnani, Caroline; Joubert, Caroline; Marie, Benjamin; Belliard, Corinne; Tayale, Alexandre; Fievet, Julie; Levy, Peva; Piquemal, David; Marin, Frederic; Le Moullac, Gilles; Ky, Chin-long; Garen, Pierre; Lo, Cedrick; Saulnier, Denis. |
| Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Biomineralization; Pinctada margaritifera. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00167/27840/26049.pdf |
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| Teaniniuraitemoana, Vaihiti; Huvet, Arnaud; Levy, Peva; Klopp, Christophe; Lhuillier, Emeline; Gaertner-mazouni, Nabila; Gueguen, Yannick; Le Moullac, Gilles. |
| Background Black pearl farming is based on culture of the blacklip pearl oyster Pinctada margaritifera (Mollusca, lophotrochozoa), a protandrous hermaphrodite species. At first maturation, all individuals are males. The female sex appears progressively from two years old, which represents a limitation for broodstock conditioning for aquaculture production. In marine mollusks displaying hermaphroditic features, data on sexual determinism and differentiation, including the molecular sex determining cascade, are scarce. To increase genomic resources and identify the molecular mechanisms whereby gene expression may act in the sexual dimorphism of P. margaritifera, we performed gonad transcriptome analysis. Results The gonad transcriptome of P. margaritifera... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Gametogenesis; Transcriptome; Differential expression; Sex determinism. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00201/31194/29593.pdf |
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| Le Moullac, Gilles; Phillip De Laborie, Loic; Saulnier, Denis; Goarant, Cyrille; Dehasque, Marleen. |
| Anderson (1992) y Raa (1996) han escrito impresionantes críticas acerca del uso de inmunoestimulantes en acuacultura. La mayoría de los trabajos citados se refieren principalmente al cultivo de peces y pocos acerca del camarón. Así que este trabajo está enfocado al camarón y las pocas pruebas publicadas. Varios de los inmunoestimulantes y su manera de actuar sobre el sistema inmune en camarones se describen brevemente, así como los métodos usados para administrar las sustancias inmunoestimulatorias para camarón. La dificultad para experimentar en este campo está señalada con algunos ejemplos. |
| Tipo: Text |
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| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00029/14011/11197.pdf |
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| Gueguen, Yannick; Czorlich, Yann; Mastail, Max; Le Tohic, Bruno; Defay, Didier; Lyonnard, Pierre; Marigliano, Damien; Gauthier, Jean-pierre; Bari, Hubert; Lo, Cedrik; Chabrier, Sebastien; Le Moullac, Gilles. |
| Cultured pearls are human creations formed by inserting a nucleus and a small piece of mantle tissue into a living shelled mollusc, usually a pearl oyster. Although many pearl observations intuitively suggest a possible rotation of the nucleated pearl inside the oyster, no experimental demonstration of such a movement has ever been done. This can be explained by the difficulty of observation of such a phenomenon in the tissues of a living animal. To investigate this question of pearl rotation, a magnetometer system was specifically engineered to register magnetic field variations with magnetic sensors from movements of a magnetic nucleus inserted in the pearl oyster. We demonstrated that a continuous movement of the nucleus inside the oyster starts after a... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pearl rotation; Biomineralization. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38598/37127.pdf |
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| Le Moullac, Gilles. |
| Summer mortality of the oyster result from the interaction of many factors. A respiratory assumption proposes a link between the supposed causes of the energy state, the cultivation methods and the medium. This work is focused towards the search for specific metabolic markers of environmental stresses. The cDNA of genes coding for PK and PEPCK were cloned and sequenced in order to detect their regulation level. The sediment nearness affects the state of energy reserves, the metabolic pathways of energy production implying a regulation of the PK. Face to the dissolved oxygen decline, oysters were oxyregulator but with a low regulation ability. In hypoxia, glycolysis was slowed down. PK activity was inhibited resulting from an increase in alanine sensitivity... |
| Tipo: Text |
Palavras-chave: Régulation; Enzyme; Expression génique; Anoxie; Bivalve. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/these-4315.pdf |
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| Menoud, Mathilde; Van Wynsberge, Simon; Le Moullac, Gilles; Levy, Peva; Andrefouet, Serge; Remoissenet, Georges; Gaertner-mazouni, Nabila. |
| Monitoring gonadmaturation for protandrous and functional hermaphrodite species such as the giant clamTridacna maxima is difficult due to the juxtaposition and relative proportion ofmale and female tissues in the gonad [gonadal sex ratio (GSR)]. Here, the relevance of the widely used gonadosomatic index (GSI) as proxy of giant clam gonad maturation is tested with a large dataset (n = 265). Gonadosomatic index is compared with other indices, namely the proportion of the male part harboring spermatozoids, the proportion of empty oocyte follicles, the mean oocyte diameter, and the oocyte elongation. At gonad scale, high index variability highlighted partial spawning. At individual scale, male and female maturation proxies were contrasted, showing either... |
| Tipo: Text |
Palavras-chave: Giant clam; Marine bivalve; Reproduction; Spawn; Tatakoto atoll; Histology; Tridacna maxima. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00333/44441/44111.pdf |
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| Le Moullac, Gilles; Soyez, Claude; Ky, Chin-long. |
| The pearl oyster is one of the rare animal models that support two distinct genomes, through the surgical graft process operated for culture pearl production. This grafted organism is assimilated to a chimera whose physiological functioning remains poorly known. The question of the energy expenditure comparison between chimera and non-chimera animals arises. To answer this question, grafted and non-grafted pearl oysters were evaluated for their energetic needs by the indirect calorimetry method. This method made it possible to measure the energy expenditure based on the respiration rate (RR) measurement, reflecting the basal metabolism. The results showed that the RR values for grafted and non-grafted pearl oysters were not significantly different... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00437/54861/56350.pdf |
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| Linard, Clementine; Gueguen, Yannick; Moriceau, Jacques; Soyez, Claude; Hui, Belinda; Raoux, Aurore; Cuif, Jean Pierre; Cochard, Jean-claude; Le Pennec, Marcel; Le Moullac, Gilles. |
| Marine mollusc shell growth has been widely measured using fluorochrome marking. In order to test the efficiency and reliability of calcein staining on P. margaritifera shells and pearls, the present study examined two administration methods, different concentrations and several immersion times.Immersion in a 150 mg.L-1 calcein solution for 12 h to 24 h appeared to be the best method for marking P. margaritifera shells. For pearl marking, injection of a 200 mg.L-1 calcein solution into the pearl pouch was the optimal method. Calcein marking was then used to measure the influence of food resource levels on the shell growth. Groups of 23-month-old P. margaritifera were fed at three trophic levels for two months. The two highest food levels tested (6 000... |
| Tipo: Text |
Palavras-chave: Bivalvia; Pearl; Shell; Growth; Scanning electron microscope. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00028/13907/11195.pdf |
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| Ky, Chin-long; Sham Koua, Manaarii; Le Moullac, Gilles. |
| Beaded cultured pearl farming is a lengthy aquaculture process, particularly when the pearl oysters are produced through a hatchery propagation system, and includes the key steps of artificial breeding, larval and spat rearing before graft operations can take place. Within its genus, Pinctada margaritifera has the ability to produce the widest range of pearl colours, thanks to the donor colour polymorphism of the inner shell, which is mainly responsible for colour transmission. As hatchery spat production in P. margaritifera leads to several colour phenotypes (at 3 months old), the aim of this study was to determine whether a relation exists between the colour of the donors as spat and the final pearl colour. In the experiment, which took place over a... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Phenotypes; Spat shell colour; Earlier selection; Pearl colour. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00418/52969/53895.pdf |
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| Aquacop, Aquacop; Le Moullac, Gilles; Damez, D. |
| The notion of penaeid shrimp postlarvae quality, which implies a survival potential, is still a subjective parameter. The zootechnical criteria and the visual observations are not sufficient to evaluate the physiological condition of the hatchery reared postlarvae. The resistance of postlarvae to salinity shocks is a representative criteria of their condition. The goal of this study is to present a non-linear regression model at 2 variables (size, salinity) for the P. vannamei postlarvae resistance to salinity shocks. Between the second and the twentieth day after metamorphosis; 5 salinity shocks (20, 15, 10, 5 and 20/00) were tested daily, each one with 50 postlarvae. Survival rates were recorded after 2 hrs. Concurrently, the postlarvae growth in the... |
| Tipo: Text |
Palavras-chave: Salinity; Resistance; Growth; Postlarvae; Penaeus vannamei; Salinité; Résistance; Croissance; Postlarve; Penaeus vannamei. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-4430.pdf |
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| Le Moullac, Gilles; Goraguer, Herle; Maihota, Mayalen; Teissier, Hinano; Tiapari, Jerome; Bennett, Auguste; Soyez, Claude. |
| Dans le cadre du programme Reproduction de l’huître perlière Pinctada margaritifera, dont l’objectif est la maîtrise des croisements, le conditionnement en laboratoire des reproducteurs est une étape nécessaire pour espérer contrôler et synchroniser la maturation de ces reproducteurs. Les facteurs présumés qui permettront de contrôler la maturation sont d’ordre environnemental : l’alimentation et la température. Cependant avant d’espérer établir la relation entre la fonction physiologique et les facteurs qui contrôlent cette fonction, il est indispensable de mettre en œuvre une zootechnie adaptée aux besoins vitaux de façon à assurer la survie et une bonne croissance. Cette étude a pour objectif de tester la survie et la croissance des huîtres perlières... |
| Tipo: Text |
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| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00182/29288/27729.pdf |
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| Latchere, Oihana; Mehn, Vincent; Gaertner-mazouni, Nabila; Le Moullac, Gilles; Fievet, Julie; Belliard, Corinne; Cabral, Philippe; Saulnier, Denis. |
| Environmental parameters, such as food level and water temperature, have been shown to be major factors influencing pearl oyster shell growth and molecular mechanisms involved in this biomineralization process. The present study investigates the effect of food level (i.e., microalgal concentration) and water temperature, in laboratory controlled conditions, on the last stages of pearl mineralization in order to assess their impact on pearl quality. To this end, grafted pearl oysters were fed at different levels of food and subjected to different water temperatures one month prior to harvest to evaluate the effect of these factors on 1) pearl and shell deposition rate, 2) expression of genes involved in biomineralization in pearl sacs, 3) nacre... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00428/54007/55223.pdf |
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| Le Moullac, Gilles; Hui, Belinda; Vonau, Vincent; Levy, Peva; Cochard, Jean-claude. |
| Preliminary experiments allowed defining basic requirements of Pinctada margaritifera for somatic and gonadic growth in controlled conditions. This study was continued by observation of the gametogenesis at a cellular level. In April/May 2008, 125 oysters were conditioned for 30 days in 25 tubular tanks (30 L). Water was renewed 4 times/hour. A mixed (v:v) diet of Isochrysis galbana (T-Iso) and Chaetoceros gracilis was supplied continuously. A control batch of 125 oysters was maintained in trays in the lagoon. An initial sample of 30 individuals was made. Gonadic changes were first characterized using a gonadal development index (GDI) based on the ratio of the gonad area to the total visceral mass area on a sagittal section of the body. Histological... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Reproduction; Gametogenesis; Broodstock. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00029/14010/11196.pdf |
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| Joubert, Caroline; Linard, Clementine; Le Moullac, Gilles; Soyez, Claude; Saulnier, Denis; Teaniniuraitemoana, Vaihiti; Ky, Chin-long; Gueguen, Yannick. |
| In this study, we analyzed the combined effect of microalgal concentration and temperature on the shell growth of the bivalve Pinctada margaritifera and the molecular mechanisms underlying this biomineralization process. Shell growth was measured after two months of rearing in experimental conditions, using calcein staining of the calcified structures. Molecular mechanisms were studied though the expression of 11 genes encoding proteins implicated in the biomineralization process, which was assessed in the mantle. We showed that shell growth is influenced by both microalgal concentration and temperature, and that these environmental factors also regulate the expression of most of the genes studied. Gene expression measurement of shell matrix protein... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00210/32095/30535.pdf |
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| Le Moullac, Gilles; Soyez, Claude; Latchere, Oihana; Vidal-dupiol, Jeremie; Fremery, Juliette; Saulnier, Denis; Lo Yat, Alain; Belliard, Corinne; Mazouni-gaertner, Nabila; Gueguen, Yannick. |
| The pearl culture is one of the most lucrative aquacultures worldwide. In many South Pacific areas, it depends on the exploitation of the pearl oyster Pinctada margaritifera and relies entirely on the environmental conditions encountered in the lagoon. In this context, assessing the impact of climatic stressors, such as global warming and ocean acidification, on the functionality of the resource in terms of renewal and exploitation is fundamental. In this study, we experimentally addressed the impact of temperature (22, 26, 30 and 34 °C) and partial pressure of carbon dioxide pCO2 (294, 763 and 2485 μatm) on the biomineralization and metabolic capabilities of pearl oysters. While the energy metabolism was strongly dependent on temperature, results showed... |
| Tipo: Text |
Palavras-chave: Global change; Pearl oyster; Bioenergetic; Biomineralization; Pacific ocean; French Polynesia. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00332/44364/43969.pdf |
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| Registros recuperados: 65 | |
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