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| Santos,Jucélia Stadinicki dos; Alfieri,Alice Fernandes; Leite,José Paulo Gagliardi; Skraba,Irene; Alfieri,Amauri Alcindo. |
| From January/2000 to December/2003, 550 diarrheic fecal samples from the children and adults were collected in several geographical regions of Paraná State, Brazil. The enzyme immunoassay showed 120 (21.8%) samples positive for the group A rotaviruses. One hundred and fourteen samples were genotyped by multiplex-nested-PCR assay. The highest frequency (77.5%) of the positive samples (n=93) was observed in the children under 5 years old. Rotavirus diarrhea was more frequent in the cold and dry seasons of the four evaluated years. The most frequent genotypes were: G1 (50.9%), G4 (9.6%), G9 (7.0%), G2 (1.7%), G3 (0.9%), P[ 8] (71.9%), and P[ 4] (3.5%). The P[ 8] G1 (46.5%) and P[ 8] G4 (9.6%) were the main combinations found to P and G genotypes. The mixed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Rotavirus; Diagnostic; PAGE; RT-PCR; Genotypes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200008 |
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| Ferreira,Thais Lourenço; Becho,Mariana Costa; Bernardo,Aline Rodrigues; Chaves,Thaís Castelo Branco; Ribeiro,Rosemary Suely; Lima,Jurandir Sampaio de; Fialho,Alexandre Madi; Leite,José Paulo Gagliardi; Mazur,Carlos; Danelli,Maria das Graças Miranda. |
| The aim of this work was to determine the performance of latex agglutination test (LAT) for evaluating children acute gastroenteritis by rotavirus. The LAT showed good sensitivity, as well as specificity and predictive positive value and due to its simplicity and speed, it has been suitable for rotavirus diagnosis in hospital laboratories. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Rotavirus; Diagnosis; Latex agglutination test; Poliacrylamide gel electrophoresis. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400035 |
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| Spano,Liliana Cruz; Ferreira,Mônica Simões Rocha; Almeida,Marilda Santos; Nascimento,Jussara Pereira do; Leite,José Paulo Gagliardi. |
| Human cytomegalovirus (HCMV) displays genetic variability in several regions, supposed to be related with strain-specific tissue tropism and immunopathogenesis. Based on sequence variation in the UL55 gene that encodes gB glycoprotein, HCMV strains can be assigned to one of four genotypes. Previous studies have addressed gB genotyping mostly by investigating strains derived from immunosuppressed patients, sometimes without previous knowledge about genotype distribution in a geographic area. The present study verified the distribution of HCMV gB genotypes of strains obtained from immunocompetent women at Vitória City, Espírito Santo State, Southeastern, Brazil. The HCMV genome was extracted from their cervical secretion, fetal and maternal placenta tissues... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytomegalovirus; RFLP; GB genotypes; Cervical secretion; Placenta tissues. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300008 |
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| Reis,Thaís Aparecida Vieira; Assis,Andrêssa Silvino Ferreira; Valle,Daniel Almeida do; Barletta,Vívian Honorato; Carvalho,Iná Pires de; Rose,Tatiana Lundgren; Portes,Silvana Augusta Rodrigues; Leite,José Paulo Gagliardi; Silva,Maria Luzia da Rosa e. |
| Abstract Human adenovirus species F (HAdV-F) type 40 and 41 are commonly associated with acute diarrheal disease (ADD) across the world. Despite being the largest state in southeastern Brazil and having the second largest number of inhabitants, there is no information in the State of Minas Gerais regarding the role of HAdV-F in the etiology of ADD. This study was performed to determine the prevalence, to verify the epidemiological aspects of infection, and to characterize the strains of human adenoviruses (HAdV) detected. A total of 377 diarrheal fecal samples were obtained between January 2007 and August 2011 from inpatient and outpatient children of age ranging from 0 to 12 years. All samples were previously tested for rotavirus, norovirus, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epidemiology of ADD; Acute diarrheal disease (ADD); Human adenovirus species F. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100243 |
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