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	Provedor de dados Genet. Mol. Biol. (2) Autor Loreto,Élgion Lucio da Silva (2) Deprá,Maríndia (1) Herédia,Fabiana (1) Rohde,Claudia (1) Sassi,Adriana Koslovski (1) Sepel,Lenira Maria Nunes (1) Valente,Vera Lucia da Silva (1) Mais... Palavra-chave Drosophila willistoni (1) GFP (1) Gypsy (1) P (1) PiggyBac (1) Transgenic Drosophila (1) Transposable element (1) Transposable elements (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2005 (1) 2004 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers Genet. Mol. Biol. Deprá,Maríndia; Sepel,Lenira Maria Nunes; Loreto,Élgion Lucio da Silva. We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation. Tipo: Info:eu-repo/semantics/article Palavras-chave: Transgenic Drosophila; PiggyBac; GFP; Transposable elements. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012 Transposable elements P and gypsy in natural populations of Drosophila willistoni Genet. Mol. Biol. Sassi,Adriana Koslovski; Herédia,Fabiana; Loreto,Élgion Lucio da Silva; Valente,Vera Lucia da Silva; Rohde,Claudia. The presence and integrity of the P transposon and the gypsy retrotransposon in the genome of 18 samples of natural Drosophila willistoni populations collected from a large area of South America were Southern blot screened using Drosophila melanogaster probes. The aim of this screening was provide further knowledge-base on the geographical distribution of D. willistoni and to carry out an inter-population analysis of the P and gypsy elements present in the genomes of the populations analyzed. The fragment patterns obtained indicate that both the P and gypsy elements are ancient in the D. willistoni genome, but whereas the gypsy retroelement appears to be invariable and stable the P element varies between populations and appears to still have some capacity... Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila willistoni; Transposable element; P; Gypsy. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500013 Registros recuperados: 2 Primeira ... 1 ... Última

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